scholarly journals Tamm-Horsfall glycoprotein in streptozotocin diabetic rats: a study of kidney in situ hybridization, immunohistochemistry, and urinary excretion

Diabetologia ◽  
1995 ◽  
Vol 38 (5) ◽  
pp. 525-535 ◽  
Author(s):  
R. Rasch ◽  
O. Torffvit ◽  
S. Bachmann ◽  
P. K. Jensen ◽  
N. O. Jacobsen
Keyword(s):  
In Situ Hybridization ◽  
Urinary Excretion ◽  
Diabetic Rats ◽  
Streptozotocin Diabetic Rats

scholarly journals Alterations in Nitric Oxide Activity and Sensitivity in Early Streptozotocin-Induced Diabetes Depend on Arteriolar Size

2000 ◽  
Vol 1 (3) ◽  
pp. 221-232 ◽  
Author(s):  
Bastiaan vanDam ◽  
Cihan Demirci ◽  
Hans J. Reitsma ◽  
Anton A. van Lambalgen ◽  
Gerard C. van den Bos ◽  
...  
Keyword(s):  
Diabetic Rats ◽  
Diabetic Microangiopathy ◽  
Basal Diameter ◽  
Early Increase ◽  
Before And After ◽  
Streptozotocin Induced Diabetes ◽  
Streptozotocin Diabetic Rats ◽  
Induced Changes ◽  
Cholinergic Vasodilatation

Changes in NO activity may play an important role in the early increase in microvascular flow that has been implicated in the pathogenesis of diabetic microangiopathy. We assessed, in thein situspinotrapezius muscle preparation of 6 weeks' streptozotocin-diabetic rats (n= 6) and of agematched controls (n= 8), basal inside diameters of A2–A4 arterioles and the reactivity to topically applied acetylcholine and nitroprusside, before and afterNG-nitro-L-arginine. In diabetic rats, cholinergic vasodilatation in A2–A4 arterioles was intact. Basal diameter in A3 and A4 arterioles was significantly higher in streptozotocin-diabetic rats. The increased basal diameter in A3 arterioles was partially due to an increased contribution of NO to basal diameter. The response to nitroprusside was impaired in streptozotocin-diabetic rats in A2, but not in A3 and A4 arterioles. Thus, this study shows that NO activity and sensitivity are altered after 6 weeks of streptozotocin-induced diabetes. These streptozotocin-induced changes are anatomically specific and, for arterioles, depend on their position within the vascular tree.

Glycogen repletion in different skeletal muscles from diabetic rats

1984 ◽  
Vol 247 (6) ◽  
pp. E740-E746 ◽  
Author(s):  
N. Hamilton ◽  
E. G. Noble ◽  
C. D. Ianuzzo
Keyword(s):  
Skeletal Muscles ◽  
Detrimental Effect ◽  
Muscular Activity ◽  
Diabetic Rats ◽  
Albino Rats ◽  
Sprague Dawley ◽  
Streptozotocin Diabetic Rats ◽  
Glycogen Repletion ◽  
Stimulation Of

It was hypothesized that chronically untreated streptozotocin-diabetic rats may compensate for the detrimental effect of insulin deficiency on glycogen restoration following muscular work. Glycogen concentration ([GLY]) was reduced by in situ stimulation of the sciatic nerve of anesthetized normal (N) and diabetic (D) Sprague-Dawley albino rats. Glycogen repletion occurred most rapidly within 30 min after stimulation. By 2 h all N muscles returned to basal [GLY]. D muscles repleted glycogen at a rate 45–75% of normal and not all of the D muscle returned to basal [GLY] by 8 h poststimulation. Neither the partial reduction in diabetic hyperglycemia by using phlorizin nor the acute further lowering of diabetic hypoinsulinemia by using insulin antibody affected glycogen restoration in D muscles. Acute insulin replenishment resulted in restoration of normal [GLY] in D muscles within 2 h poststimulation. These findings are not consistent with the proposed hypothesis and indicate insulin is necessary to have normal rates of glycogen restoration following muscular activity.

Elevated urinary excretion of endothelins in streptozotocin diabetic rats

Life Sciences ◽  
1994 ◽  
Vol 54 (11) ◽  
pp. PL197-PL200 ◽  
Author(s):  
E. Morabito ◽  
N. Corsico ◽  
S. Serafini ◽  
E.Arrigoni Martelli
Keyword(s):  
Urinary Excretion ◽  
Diabetic Rats ◽  
Streptozotocin Diabetic Rats

scholarly journals Renal protective effect of chronic inhibition of COX-2 with SC-58236 in streptozotocin-diabetic rats

2011 ◽  
Vol 300 (6) ◽  
pp. H2316-H2322 ◽  
Author(s):  
J. Quilley ◽  
M. Santos ◽  
P. Pedraza
Keyword(s):  
Urinary Excretion ◽  
Structural Changes ◽  
Weight Ratio ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Protective Effects ◽  
Renal Protection ◽  
Tnf Α ◽  
Streptozotocin Diabetic Rats ◽  
Cox 2

The induction of renal cyclooxygenase-2 (COX-2) in diabetes has been implicated in the renal functional and structural changes in models where hypertension or uninephrectomy was superimposed. We examined the protective effects of 3 mo treatment of streptozotocin-diabetic rats with a highly selective COX-2 inhibitor (SC-58236) in terms of albuminuria, renal hypertrophy, and the excretion of TNF-α and TGF-β, which have also been implicated in the detrimental renal effects of diabetes. SC-58236 treatment (3 mg·kg−1·day−1) of diabetic rats resulted in reduced urinary excretion of PGE2, 6-ketoPGF1α, and thromboxane B2, all of which were increased in the diabetic rat compared with age-matched nondiabetic rats. However, serum thromboxane B2 levels were unchanged, confirming the selectivity of SC-58236 for COX-2. The renal protective effects of treatment of diabetic rats with the COX-2 inhibitor were reflected by a marked reduction in albuminuria, a reduction in kidney weight-to-body weight ratio, and TGF-β excretion and a marked decrease in the urinary excretion of TNF-α. The protective effects of SC-58236 were independent of changes in plasma glucose levels or serum advanced glycation end-product levels, which were not different from those of untreated diabetic rats. In an additional study, the inhibition of COX-2 with SC-58236 for 4 wk in diabetic rats resulted in creatinine clearance rates not different from those of control rats. These results confirm that the inhibition of COX-2 in the streptozotocin-diabetic rat confers renal protection and suggest that the induction of COX-2 precedes the increases in cytokines, TNF-α, and TGF-β.

DNA sequence mapping in interphase and metaphase chromosomes by fluorescence in situ hybridization

1992 ◽  
Vol 50 (1) ◽  
pp. 496-497
Author(s):  
Barbara Trask ◽  
Susan Allen ◽  
Anne Bergmann ◽  
Mari Christensen ◽  
Anne Fertitta ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Dna Sequence ◽  
Dna Sequences ◽  
Dual Band ◽  
Nick Translation ◽  
Metaphase Chromosomes ◽  
Band Pass ◽  
Texas Red ◽  
Fluorescent Spot

Using fluorescence in situ hybridization (FISH), the positions of DNA sequences can be discretely marked with a fluorescent spot. The efficiency of marking DNA sequences of the size cloned in cosmids is 90-95%, and the fluorescent spots produced after FISH are ≈0.3 μm in diameter. Sites of two sequences can be distinguished using two-color FISH. Different reporter molecules, such as biotin or digoxigenin, are incorporated into DNA sequence probes by nick translation. These reporter molecules are labeled after hybridization with different fluorochromes, e.g., FITC and Texas Red. The development of dual band pass filters (Chromatechnology) allows these fluorochromes to be photographed simultaneously without registration shift.

Ultrastructural analysis of the spatial distribution of MRNA

1992 ◽  
Vol 50 (1) ◽  
pp. 552-553
Author(s):  
Gary Bassell ◽  
Robert H. Singer
Keyword(s):  
Electron Microscopy ◽  
Spatial Distribution ◽  
In Situ Hybridization ◽  
High Resolution ◽  
Nucleic Acids ◽  
Colloidal Gold ◽  
Dna Probe ◽  
Nucleotide Analogs ◽  
Gold Particles

We have been investigating the spatial distribution of nucleic acids intracellularly using in situ hybridization. The use of non-isotopic nucleotide analogs incorporated into the DNA probe allows the detection of the probe at its site of hybridization within the cell. This approach therefore is compatible with the high resolution available by electron microscopy. Biotinated or digoxigenated probe can be detected by antibodies conjugated to colloidal gold. Because mRNA serves as a template for the probe fragments, the colloidal gold particles are detected as arrays which allow it to be unequivocally distinguished from background.

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