scholarly journals Ca2+-activated K+ channels from an insulin-secreting cell line incorporated into planar lipid bilayers

Diabetologia ◽  
1992 ◽  
Vol 35 (7) ◽  
pp. 619-623 ◽  
Author(s):  
Y. Oosawa ◽  
S. J. H. Ashcroft ◽  
F. M. Ashcroft
Keyword(s):  
Cell Line ◽  
Lipid Bilayers ◽  
Planar Lipid Bilayers ◽  
Secreting Cell ◽  
K Channels

scholarly journals Potassium blocks barium permeation through a calcium-activated potassium channel.

1988 ◽  
Vol 92 (5) ◽  
pp. 549-567 ◽  
Author(s):  
J Neyton ◽  
C Miller
Keyword(s):  
Skeletal Muscle ◽  
Lipid Bilayers ◽  
Dissociation Rate ◽  
Planar Lipid Bilayers ◽  
Rat Skeletal Muscle ◽  
K Channels ◽  
Calcium Activated Potassium Channel ◽  
Micromolar Range ◽  
High Conductance ◽  
External K

Single high-conductance Ca2+-activated K+ channels from rat skeletal muscle were inserted into planar lipid bilayers, and discrete blocking by the Ba2+ ion was studied. Specifically, the ability of external K+ to reduce the Ba2+ dissociation rate was investigated. In the presence of 150 mM internal K+, 1-5 microM internal Ba2+, and 150 mM external Na+, Ba2+ dissociation is rapid (5 s-1) in external solutions that are kept rigorously K+ free. The addition of external K+ in the low millimolar range reduces the Ba2+ off-rate 20-fold. Other permeant ions, such as Tl+, Rb+, and NH4+ show a similar effect. The half-inhibition constants rise in the order: Tl+ (0.08 mM) less than Rb+ (0.1 mM) less than K+ (0.3 mM) less than Cs+ (0.5 mM) less than NH4+ (3 mM). When external Na+ is replaced by 150 mM N-methyl glucamine, the Ba2+ off-rate is even higher, 20 s-1. External K+ and other permeant ions reduce this rate by approximately 100-fold in the micromolar range of concentrations. Na+ also reduces the Ba2+ off-rate, but at much higher concentrations. The half-inhibition concentrations rise in the order: Rb+ (4 microM) less than K+ (19 microM) much less than Na+ (27 mM) less than Li+ (greater than 50 mM). The results require that the conduction pore of this channel contains at least three sites that may all be occupied simultaneously by conducting ions.

The expression and regulation of depolarization-activated K+ channels in the insulin-secreting cell line INS-1

2001 ◽  
Vol 442 (1) ◽  
pp. 49-56 ◽  
Author(s):  
Jiping Su ◽  
Huang Yu ◽  
Nibedita Lenka ◽  
Jürgen Hescheler ◽  
Susanne Ullrich
Keyword(s):  
Cell Line ◽  
Secreting Cell ◽  
K Channels

scholarly journals Ca2+-dependent K+channels from rat olfactory cilia characterized in planar lipid bilayers

FEBS Letters ◽  
2005 ◽  
Vol 579 (7) ◽  
pp. 1675-1682 ◽  
Author(s):  
Karen Castillo ◽  
Juan Bacigalupo ◽  
Daniel Wolff
Keyword(s):  
Lipid Bilayers ◽  
Planar Lipid Bilayers ◽  
Olfactory Cilia ◽  
K Channels

Role of tyrosine kinase in insulin release in an insulin secreting cell line (INS-1)

1995 ◽  
Vol 7 (5) ◽  
pp. 505-512 ◽  
Author(s):  
E.J. Verspohl ◽  
B. Tollkühn ◽  
H. Kloss
Keyword(s):  
Tyrosine Kinase ◽  
Cell Line ◽  
Insulin Release ◽  
Secreting Cell
Sign in / Sign up

Export Citation Format

Share Document