Isolation and identification of a new, naturally occurring cytokinin (6-benzylaminopurineriboside) from an anise cell culture (Pimpinella anisum L.)

Planta ◽  
1983 ◽  
Vol 159 (3) ◽  
pp. 222-225 ◽  
Author(s):  
D. Ernst ◽  
W. Sch�fer ◽  
D. Oesterhelt
Keyword(s):  
Cell Culture ◽  
Isolation And Identification ◽  
Pimpinella Anisum ◽  
Naturally Occurring

Isolation and identification of plant growth-promoting bacteria associated with tall fescue

2009 ◽  
pp. 211-216 ◽  
Author(s):  
J. Monk ◽  
E. Gerard ◽  
S. Young ◽  
K. Widdup ◽  
M. O'Callaghan
Keyword(s):  
New Zealand ◽  
Plant Growth ◽  
Tall Fescue ◽  
Festuca Arundinacea ◽  
Plant Growth Promoting Bacteria ◽  
Beneficial Bacteria ◽  
Isolation And Identification ◽  
Naturally Occurring ◽  
Plant Growth Promoting ◽  
Growth Promoting

Tall fescue (Festuca arundinacea) is a useful alternative to ryegrass in New Zealand pasture but it is slow to establish. Naturally occurring beneficial bacteria in the rhizosphere can improve plant growth and health through a variety of direct and indirect mechanisms. Keywords: rhizosphere, endorhiza, auxin, siderophore, P-solubilisation

Comparison of Cell Culture and a Poliovirus Gene Probe Assay for the Detection of Enteroviruses in Environmental Water Samples

1993 ◽  
Vol 27 (3-4) ◽  
pp. 311-314 ◽  
Author(s):  
Aaron B. Margolin ◽  
Charles P. Gerba ◽  
Kenneth J. Richardson ◽  
Jaime E. Naranjo
Keyword(s):  
Cell Culture ◽  
Activated Sludge ◽  
Water Samples ◽  
Cdna Probe ◽  
Culture Method ◽  
Tidal River ◽  
Nucleic Acid Hybridization ◽  
Treated Wastewater ◽  
Gene Probe ◽  
Naturally Occurring

Nucleic acid hybridization provides a rapid non-cell culture method for the detection of enteric viruses in water. The purpose of this work was to compare the detection of naturally occurring enteroviruses by cell culture with their detection by a poliovirus gene probe in various types of water samples. Samples of activated sludge effluent, tertiary treated wastewater (activated sludge, filtration and passage through reverse osmosis), ground water, surface water and tidal river water were processed through 1 MDS Virozorb filters to concentrate any naturally occurring virus. Viruses were eluted from the filters with pH 9.5 beef extract and reduced in volume by flocculation to 20-30 ml. These concentrates were then assayed in the BGM cell line by the cytopathogenic effects (CPE) method and by a poliovirus cDNA probe (base pairs 115-7440) labeled with 32P. A total of 233 samples were assayed in this manner. In slightly more than 93% of the samples gene probe and cell culture yielded the same results. Of these samples 36 were positive by gene probe and 28 by cell culture assay. Positive samples for gene probe were confirmed by treatment with NaOH or RNAse and then reprobed. Samples demonstrating CPE upon primary passage were confirmed positive by subsequent passage of cell lysate on a new monolayer of BGM cells. Ten samples were positive by gene probe and negative by cell culture, and 4 samples were negative by gene probe and positive by cell culture.

Endogenous cytokinins during embryogenesis in an anise cell culture (Pimpinella anisum L.)

Planta ◽  
1984 ◽  
Vol 161 (3) ◽  
pp. 240-245 ◽  
Author(s):  
D. Ernst ◽  
D. Oesterhelt ◽  
W. Sch�fer
Keyword(s):  
Cell Culture ◽  
Pimpinella Anisum ◽  
Endogenous Cytokinins

Isolation of naturally occurring novel isoflavonoids: an update

2019 ◽  
Vol 36 (8) ◽  
pp. 1156-1195 ◽  
Author(s):  
Nawaf Al-Maharik
Keyword(s):  
Isolation And Identification ◽  
Naturally Occurring ◽  
Leguminous Species

This review covers the literature concerning the isolation and identification of new naturally occurring isoflavonoids from Leguminosae and non-Leguminous species between 2012–2017.

scholarly journals Cell Culture of Sporadic Hepatitis E Virus in China

1999 ◽  
Vol 6 (5) ◽  
pp. 729-733 ◽  
Author(s):  
Rutong Huang ◽  
Derong Li ◽  
Shaojing Wei ◽  
Qinghong Li ◽  
Xitong Yuan ◽  
...  
Keyword(s):  
Cell Culture ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Biological Properties ◽  
Isolation And Identification ◽  
Successful Isolation ◽  
Human Lung Carcinoma ◽  
A Cell ◽  
Human Lung Carcinoma Cell ◽  
Relationship Of

ABSTRACT The isolation and identification of the 87A strain of epidemic hepatitis E virus (HEV) by means of cell culturing have been described previously. This paper reports the successful isolation of a sporadic HEV strain (G93-2) in human lung carcinoma cell (A549) cultures. The etiology, molecular and biological properties, and serological relationship of this new strain to other, epidemic HEV strains are described. The propagation of both sporadic and epidemic HEV strains in a cell culture system will facilitate vaccine research.

scholarly journals Isolation and identification of polar auxin transport inhibitors from Saussurea costus and Atractylodes japonica

2017 ◽  
Vol 70 (3) ◽  
Author(s):  
Yuta Toda ◽  
Hideyuki Shigemori ◽  
Junichi Ueda ◽  
Kensuke Miyamoto
Keyword(s):  
Auxin Transport ◽  
Polar Auxin Transport ◽  
Inhibitory Effects ◽  
Medicinal Species ◽  
Isolation And Identification ◽  
Naturally Occurring ◽  
Structure Activity ◽  
Atractylodes Japonica ◽  
Transport Inhibitors ◽  
Dehydrocostus Lactone

An intensive survey of naturally-occurring regulators of polar auxin transport (PAT) was conducted in two oriental medicinal species from the Asteraceae, <em>Saussurea costus</em> and <em>Atractylodes japonica</em>, using the radish hypocotyl bioassay system and physicochemical analyses. Costunolide and santamarine were identified as well as dehydrocostus lactone from <em>S. costus</em> roots, and atractylenolide II and (+)-eudesma-4(14),7(11)-dien-8-one from <em>Atractylodes japonica</em> rhizomes as physiologically novel compounds possessing inhibitory activities of PAT. Costunolide and santamarine showed ca. 40% inhibition of PAT in the radish hypocotyl segments at a dose of 2.5 μg/plant and 1 μg/plant, respectively. Inhibitory effects of atractylenolide II and (+)-eudesma-4(14),7(11)-dien-8-one were ca. 10 times lower than those of costunolide and santamarine. Structure–activity relationships and possible mechanisms to inhibit PAT are also discussed.

scholarly journals An update on the detection methods of Parachlamydia acanthamoebae, an atypical agent of pneumonia

2019 ◽  
pp. 86-100
Author(s):  
Avinash Rames
Keyword(s):  
Cell Culture ◽  
In Situ Hybridization ◽  
Molecular Techniques ◽  
Detection Methods ◽  
Pathogenic Role ◽  
Chain Reaction ◽  
Isolation And Identification ◽  
Polymerase Chain ◽  
Koch Postulates

Parachlamydia acanthamoebae (P. acanthamoebae) has been recognized as an emerging agent of pneumonia as it has been identified in human samples via culture-based, molecular and serological techniques. Additionally, studies on animal models have shown that it fulfills the third and fourth Koch postulates to be assigned a pathogenic role. Due to the threat posed by it, multiple tools have been employed in the search for P. acanthamoebae. The methods utilized for its detection would be cell culture based approaches which involve both animal and amoebal cell culture and also molecular techniques that encompasses polymerase chain reaction (PCR), fluorescence in situ hybridization (FISH) and in situ hybridization (ISH). Additionally, immunohistochemistry (IHC) and serology based techniques such as direct and indirect immunofluorescence are also employed with the usage of Western blotting or immunoblotting as confirmatory procedures. This review attempts to describe the variety of techniques that are present in literature for the isolation and identification of P. acanthamoebae.

scholarly journals Identification of anti-severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) oxysterol derivatives in vitro

2021 ◽  
Author(s):  
Hirofumi Ohashi ◽  
Feng Wang ◽  
Frank Stappenbeck ◽  
Kana Tsuchimoto ◽  
Chisa Kobayashi ◽  
...  
Keyword(s):  
Cell Culture ◽  
Severe Acute Respiratory Syndrome ◽  
Antiviral Activity ◽  
Viral Replication ◽  
Cultured Cells ◽  
Plasma Concentrations ◽  
Hepatitis D ◽  
Infection Assay ◽  
Naturally Occurring ◽  
Increased Risk

AbstractDevelopment of effective antiviral drugs targeting the severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) are urgently needed to combat the coronavirus disease 2019 (COVID-19). Oxysterols, defined as oxidized derivatives of cholesterol, include endogenous (naturally occurring) cholesterol metabolites as well as semi-synthetic oxysterol derivatives. We have previously studied the use of semi-synthetic oxysterol derivatives as drug candidates for inhibition of cancer, fibrosis, and bone regeneration. In this study, we have screened a panel of naturally occurring and semi-synthetic oxysterol derivatives for anti-SARS-CoV-2 activity, using a cell culture infection assay. We show that the natural oxysterols, 7-ketocholesterol, 22(R)-hydroxycholesterol, 24(S)-hydroxycholesterol, and 27-hydroxycholesterol, substantially inhibited SARS-CoV-2 propagation in cultured cells. Among semi-synthetic oxysterols, Oxy186 displayed antiviral activity comparable to natural oxysterols. In addition, related oxysterol analogues Oxy210 and Oxy232 displayed more robust anti-SARS-CoV-2 activities, reducing viral replication more than 90% at 10 μM and 99% at 15 μM, respectively. When orally administered in mice, peak plasma concentrations of Oxy210 fall into a therapeutically relevant range (19 μM), based on the dose-dependent curve for antiviral activity in our cell culture infection assay. Mechanistic studies suggest that Oxy210 reduced replication of SARS-CoV-2 with disrupting the formation of double membrane vesicles (DMVs), intracellular membrane compartments associated with viral replication. Oxy210 also inhibited the replication of hepatitis C virus, another RNA virus whose replication is associated with DMVs, but not the replication of the DMV-independent hepatitis D virus. Our study warrants further evaluation of Oxy210 and Oxy232 as a safe and reliable oral medication, which could help protect vulnerable populations with increased risk developing COVID-19.

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