The immobility of pectic substances in injured tomato leaves and its bearing on the identity of the wound hormone

Planta ◽  
1985 ◽  
Vol 165 (2) ◽  
pp. 269-276 ◽  
Author(s):  
E. A.-H. Baydoun ◽  
S. C. Fry
Keyword(s):  
Pectic Substances ◽  
Wound Hormone

Selective Staining of Acid Mucopolysaccharides By Ruthenium Red

1968 ◽  
Vol 26 ◽  
pp. 38-39
Author(s):  
J. H. Luft
Keyword(s):  
Electron Microscope ◽  
Light Microscopy ◽  
Light Microscope ◽  
Osmium Tetroxide ◽  
Single Cells ◽  
Ruthenium Red ◽  
Collagen Fibers ◽  
Selective Staining ◽  
Pectic Substances ◽  
Solid Tissues

Ruthenium red is one of the few completely inorganic dyes used to stain tissues for light microscopy. This novelty is enhanced by ignorance regarding its staining mechanism. However, its continued usefulness in botany for demonstrating pectic substances attests to selectivity of some sort. Whether understood or not, histochemists continue to be grateful for small favors.Ruthenium red can also be used with the electron microscope. If single cells are exposed to ruthenium red solution, sufficient mass can be bound to produce observable density in the electron microscope. Generally, this effect is not useful with solid tissues because the contrast is wasted on the damaged cells at the block surface, with little dye diffusing more than 25-50 μ into the interior. Although these traces of ruthenium red which penetrate between and around cells are visible in the light microscope, they produce negligible contrast in the electron microscope. However, its presence can be amplified by a reaction with osmium tetroxide, probably catalytically, to be easily visible by EM. Now the density is clearly seen to be extracellular and closely associated with collagen fibers (Fig. 1).

scholarly journals THE ISOLATION OF PECTIC SUBSTANCES FROM WOOD. II

1937 ◽  
Vol 121 (1) ◽  
pp. 165-174
Author(s):  
Ernest Anderson ◽  
L.W. Seigle ◽  
Paul W. Krznarich ◽  
Llewellyn Richards ◽  
W.W. Marteny
Keyword(s):  
Pectic Substances

Model studies on behaviour of pectic substances in the potato cell wall during boiling

1976 ◽  
Vol 19 (3) ◽  
pp. 289-303 ◽  
Author(s):  
M. J. H. Keijbets ◽  
W. Pilnik ◽  
J. F. A. Vaal
Keyword(s):  
Cell Wall ◽  
Model Studies ◽  
Pectic Substances ◽  
Potato Cell

scholarly journals Phytaspase-mediated precursor processing and maturation of the wound hormone systemin

2017 ◽  
Vol 218 (3) ◽  
pp. 1167-1178 ◽  
Author(s):  
Roman E. Beloshistov ◽  
Konrad Dreizler ◽  
Raisa A. Galiullina ◽  
Alexander I. Tuzhikov ◽  
Marina V. Serebryakova ◽  
...  
Keyword(s):  
Precursor Processing ◽  
Wound Hormone
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