The electrochemical proton gradient and its influence on citrate uptake in tonoplast vesicles of Hevea brasiliensis

Planta ◽  
1981 ◽  
Vol 153 (5) ◽  
pp. 486-493 ◽  
Author(s):  
Bernard Marin ◽  
J. Andrew C. Smith ◽  
Ulrich L�ttge
1985 ◽  
Vol 229 (2) ◽  
pp. 459-467 ◽  
Author(s):  
B P Marin

The relationship between tonoplast-bound ATPase activity and the magnitude of the electrochemical proton gradient has been investigated on tightly sealed vesicles prepared from rubber-tree (Hevea brasiliensis) latex. A variety of methods have been used to modify, either alone or together, the two components of the electrochemical proton gradient (delta mu H+). When the delta pH component was decreased either by titration with (NH4)2SO4 or by addition of protonophores or nigericin in the presence of K+, ATPase activity was stimulated. On the other hand, when the delta psi component was decreased either by addition of lipophilic cations or by addition of valinomycin in the presence of K+, ATPase activity decreased. It is concluded that activity of the tonoplast-bound ATPase is regulated by changes in the electrochemical proton gradient across the tonoplast, so that, once the maximum proton gradient is established across the tonoplast, any perturbation of the equilibrium state should result in the increased rate of ATP hydrolysis as the enzyme attempts to re-establish the initial gradient.


FEBS Letters ◽  
1974 ◽  
Vol 49 (2) ◽  
pp. 203-207 ◽  
Author(s):  
Rita Casadio ◽  
Assunta Baccarini Melandri ◽  
Davide Zannoni ◽  
Bruno A. Melandri

1981 ◽  
Vol 113 (3) ◽  
pp. 499-506 ◽  
Author(s):  
Mohammed BENYOUCEF ◽  
Jean-Louis RIGAUD ◽  
Gerard LEBLANC

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