The release of ?-amylase through gibberellin-treated barley aleurone cell walls

Planta ◽  
1987 ◽  
Vol 172 (2) ◽  
pp. 155-161 ◽  
Author(s):  
F. Gubler ◽  
A. E. Ashford ◽  
J. V. Jacobsen
Keyword(s):  
Cell Walls ◽  
Aleurone Cell ◽  
Barley Aleurone

Studies on the release of barley aleurone cell proteins: Autoradiography

Planta ◽  
1974 ◽  
Vol 119 (3) ◽  
pp. 207-220 ◽  
Author(s):  
Ru-fang Chen ◽  
Russell L. Jones
Keyword(s):  
Aleurone Cell ◽  
Barley Aleurone

Observations on the Aleurone Layer. II. Fluorescence Microscopy of the Aleurone-Sub-Aleurone Junction With Emphasis on Possible β-1,3-Glucan Deposits in Barley

1977 ◽  
Vol 4 (6) ◽  
pp. 917 ◽  
Author(s):  
RG Fulcher ◽  
G Setterfield ◽  
ME Mccully ◽  
PJ Wood
Keyword(s):  
Hordeum Vulgare ◽  
Fluorescence Microscopy ◽  
Cell Walls ◽  
Structural Organization ◽  
Periodate Oxidation ◽  
Aleurone Layer ◽  
Hordeum Vulgare L ◽  
Aleurone Cell ◽  
Tissue Sections ◽  
Primary Substrate

The structural organization of the cell walls at the aleurone-sub-aleurone junction has been examined by fluorescence microscopy in ungerminated seeds of two varieties of Hordeum vulgare L. (cultivars Himalaya and Vanier). The sub-aleurone cell walls that are immediately adjacent to the aleurone layer are considerably thicker than the remainder of endosperm walls and contain extensive deposits of aniline blue-positive material. The latter was not significantly affected by periodate oxidation and was removed from tissue sections only by β-1,3-glucanases or hot dimethyl sulphoxide. These deposits may represent the primary substrate for endo-β-1,3-glucanases secreted by the aleurone layer during germination.

Release of Ferulic Acid Esters From Barley Aleurone. I. Time Course of Gibberellic-Acid-Induced Release From Isolated Layers

1985 ◽  
Vol 12 (3) ◽  
pp. 297 ◽  
Author(s):  
F Gubler ◽  
AE Ashford
Keyword(s):  
Gibberellic Acid ◽  
Ferulic Acid ◽  
Cell Walls ◽  
Time Course ◽  
Free Acid ◽  
Hordeum Vulgare L ◽  
Barley Aleurone ◽  
Ester Linkage ◽  
Hydrolysis Of ◽  
Acid Esters

Removal of ferulic acid from barley aleurone cell walls is closely correlated with wall hydrolysis. The ferulic acid content of isolated aleurone layers (Hordeum vulgare L. cv. Himalaya) falls progressively during incubation with gibberellic acid (GA3), to about one-third of the original content, by 72 h. At the same time an equivalent amount accumulates in the incubation medium, not as the free acid but still esterified to other components. The data show that there is no modification of the feruloyl group, or hydrolysis of the ester linkage prior to removal from the tissue, and therefore the GA3-induced aleurone peroxidases and esterases are not directly involved.

Role of aleurone cell walls in water diffusion and distribution within cereal grains

2020 ◽  
Vol 93 ◽  
pp. 102952 ◽  
Author(s):  
Ruifeng Ying ◽  
Tong Li ◽  
Caie Wu ◽  
Meigui Huang
Keyword(s):  
Cell Walls ◽  
Water Diffusion ◽  
Cereal Grains ◽  
Aleurone Cell

Gibberellic acid, ?-1,3-glucanase and the cell walls of barley aleurone layers

Planta ◽  
1970 ◽  
Vol 92 (1) ◽  
pp. 73-84 ◽  
Author(s):  
Lincoln Taiz ◽  
Russell L. Jones
Keyword(s):  
Gibberellic Acid ◽  
Cell Walls ◽  
Barley Aleurone

Resistance of wheat aleurone cell walls to acid and xylanase action

2001 ◽  
Vol 158 (1) ◽  
pp. 21-27 ◽  
Author(s):  
Jorge E. Morales ◽  
Irma Bernal-Lugo ◽  
Carlos Argüello ◽  
Jaime Ortega-Lopez ◽  
Refugio Rodríguez-Vázquez ◽  
...  
Keyword(s):  
Cell Walls ◽  
Aleurone Cell

Aleurone Cell Walls of Wheat Grain: High Spatial Resolution Investigation Using Synchrotron Infrared Microspectroscopy

2008 ◽  
Vol 62 (8) ◽  
pp. 895-900 ◽  
Author(s):  
F. Jamme ◽  
P. Robert ◽  
B. Bouchet ◽  
L. Saulnier ◽  
P. Dumas ◽  
...  
Keyword(s):  
Spatial Resolution ◽  
Cell Walls ◽  
High Spatial Resolution ◽  
Wheat Grain ◽  
Aleurone Cell ◽  
Infrared Microspectroscopy

Barley aleurone xylanase: its biosynthesis and possible role

1989 ◽  
Vol 67 (2) ◽  
pp. 297-302 ◽  
Author(s):  
E. Benjavongkulchai ◽  
M. S. Spencer
Keyword(s):  
Electron Microscopy ◽  
Cell Walls ◽  
De Novo ◽  
Xylanase Activity ◽  
Hordeum Vulgare L ◽  
Aleurone Cells ◽  
Initial Release ◽  
Barley Aleurone ◽  
Scanning Electron

The synthesis of barley (Hordeum vulgare L. cv. Himalaya) aleurone xylanase was found to be dependent on both gibberellic acid (GA3) and Ca2+, but inhibited by cycloheximide and cordycepin. Studies using density labeling of barley aleurone layers showed that xylanase was synthetized de novo in response to GA3 and Ca2+. Neither GA3 nor Ca2+ alone induced a large increase in xylanase activity. The concentration of Ca2+ required for maximum xylanase induction was 5 – 40 mM. Xylanase activity was found to develop simultaneously with that of α-amylase in the incubation medium during the first 24 h of incubation with GA3. A critical point with respect to the role of xylanase is the extent of its activity by the time of the initial release of α-amylase. The release of α-amylase into the medium was detectable at 6 h. From 2 to 6% of the cell wall was hydrolysed by xylanase after incubation for 6 h, which was probably sufficient to permit the release of α-amylase. Scanning electron microscopy showed that the purified barley aleurone xylanase hydrolysed the cell walls of barley aleurone layers in the absence of GA3. It is likely that xylanase plays an important role in the release of enzymes from aleurone cells.

Studies on the release of barley aleurone cell proteins: Kinetics of labelling

Planta ◽  
1974 ◽  
Vol 119 (3) ◽  
pp. 193-206 ◽  
Author(s):  
Ru-fang Chen ◽  
Russell L. Jones
Keyword(s):  
Aleurone Cell ◽  
Barley Aleurone ◽  
Kinetics Of
Sign in / Sign up

Export Citation Format

Share Document