Determination of endogenous abscisic acid levels in immature cereal embryos during in vitro culture

Planta ◽  
1988 ◽  
Vol 173 (1) ◽  
pp. 110-116 ◽  
Author(s):  
P. C. Morris ◽  
E. W. Weiler ◽  
S. E. Maddock ◽  
M. G. L. Jones ◽  
J. R. Lenton ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
In Vitro Culture ◽  
Endogenous Abscisic Acid ◽  
Cereal Embryos

scholarly journals Influence of Indole acetic acid and Tryptophan on production of Vinblastine and Vincristine of Catharanthus roseous callus cells in the accumulation media of In Vitro tissue culture

2010 ◽  
Vol 7 (3) ◽  
pp. 1113-1119
Author(s):  
Baghdad Science Journal
Keyword(s):  
Tissue Culture ◽  
High Performance Liquid Chromatography ◽  
Acetic Acid ◽  
Liquid Chromatography ◽  
In Vitro Culture ◽  
High Performance ◽  
Indole Acetic Acid ◽  
Culture Technique

This study on the plant of Ain –AL Bason Catharanthus roseous showed the ability of callus cells that is produced by In Vitro culture technique and transformed to the accumulated media (MS 40gm/L sucrose ,2gm/L IAA Indole acetic acid , 0.5gm/L Tryptophan) to produce Vinblastine and Vincristine compounds. Extraction, purification and quantitive determination of Vinblastine and Vincristine compounds using High performance liquid chromatography technique (HPLC)were carried out. The results showed that the highest concentration of Vinblastine and Vincristine compounds were ( 4.653,12.5 (ppm /0.5 dry Wight respectively from transformed callus cells from MS 40 gm /L sucrose , 2 gm / L NAA Naphthaline acetic acid .

302 REPLACEMENT OF FETAL BOVINE SERUM WITH KNOCKOUT SERUM REPLACER AND STEM CELLS CONDITIONED MEDIUM DURING IN VITRO CULTURE OF BOVINE EMBRYOS

2010 ◽  
Vol 22 (1) ◽  
pp. 307
Author(s):  
M. M. Souza ◽  
N. Z. Saraiva ◽  
C. S. Oliveira ◽  
T. A. D. Tetzner-Nanzeri ◽  
R. Vantini ◽  
...  
Keyword(s):  
Stem Cells ◽  
In Vitro Culture ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Protein Supplementation ◽  
Control Group ◽  
Bovine Embryos ◽  
Fetal Bovine

The use of fetal bovine serum (FBS) as protein supplementation in IVP of bovine embryos has presented difficulties because it can introduce a number of pathogenic components in culture systems, can be related to the birth of calf with abnormal growth and development, and precludes the establishment of the actual nutritional needs of the embryo, because it contains an unlimited variety of substances. This study evaluated the replacement of the FBS in the medium of in vitro culture (IVC) of bovine embryos, using the knockout serum replacer (KSR) as protein supplementation and culture medium conditioned with stem cells. Therefore, bovine oocytes from ovaries of slaughterhouse were selected and matured in vitro in TCM-199 medium supplemented with 10% FBS (Crypion), 1.0 μg mL-1 FSH (Pluset®, Calier, Barcelona, Spain), 50 μg mL-1 hCG (Profasi®, Serono, Geneva, Switzerland), 1.0 μg mL-1 estradiol (Sigma E-2758, Sigma Chemical, St. Louis, MO, USA), 0.2 mM sodium pyruvate, and 83.4 μg mL-1 amikacin for 24 h. After that, 1144 oocytes were fertilized in IVF-TALP medium containing 6 mg mL-1 of BSA. After 18 to 22 h, the zygotes were cultured in SOF + 5% FBS (group 2); SOF + 5% KSR (group 3); SOF (5% FBS) + 10% SOF (5% FBS) conditioned by stem cells (group 4); or SOF (5% KSR) + 10% SOF (5% KSR) conditioned by stem cells (group 5), in an atmosphere of 5% O2 at 38.5°C for 8 days. A control group outside the controlled atmosphere was added, supplemented with 5% FBS (group 1). The SOF medium supplemented with 5% FBS or KSR was conditioned by stem cells and added to SOF medium for the culture of embryo at a concentration of 10%. The rates of cleavage and production of blastocysts were assessed 48 hours and 7 days after IVF, respectively, and analyzed by chi-square test, with a significance level of 5% in the statistical program Minitab® (release 14.1, Minitab, State College, PA, USA). On the eighth day, the TUNEL test for determination of the percentage of apoptosis and the differential staining technique for determination of inner cell mass (ICM) and trophoblast (TF) were performed. The results were submitted to ANOVA, followed by comparing the means by Tukey’s test using the program GraphPad Prism (GraphPad, San Diego, CA, USA). The treatments did not differ in the production of embryos, being similar to the control group: G1 = 31.75% (74/233), G2 = 35.26% (79/224), G3 = 32.70% (74/226), G4 = 28.76% (63/219), and G5 = 26.85% (65/242). With regard to the assessment of embryonic quality, the treatments showed similar results to the control groups. No differences were observed among groups both in color and ICM/TF ratio (G1 = 0.60, G2 = 0.62, G3 =0.65, G4 = 0.60, and G5 = 0.60). Furthermore, the TUNEL showed no significant difference in the percentage of apoptosis among groups (G1 = 7.10%, G2 = 3.76%, G3 = 5.58%, G4 = 4.50%, and G5 = 4.11%). The data obtained so far indicate that it is possible to produce embryos in vitro by replacing the FBS in the culture, achieving results similar to those obtained with serum. Financial support: FAPESP 2007/58506-6.

scholarly journals Involvement of aba in flower induction of Pharbitis nil

2011 ◽  
Vol 80 (1) ◽  
pp. 21-26 ◽  
Author(s):  
Emilia Wilmowicz ◽  
Kamil Frankowski ◽  
Paulina Glazińska ◽  
Jacek Kęsy ◽  
Jan Kopcewicz
Keyword(s):  
Abscisic Acid ◽  
Red Light ◽  
Inhibitory Effect ◽  
Flower Induction ◽  
Pharbitis Nil ◽  
Endogenous Abscisic Acid ◽  
Flower Inhibition ◽  
Aba Content ◽  
Floral Bud

<p>Flowering of plants is controlled by hormones among which both stimulators and inhibitors are present. The role of abscisic acid (ABA) in flower induction of the short day plant <em>Pharbitis nil</em> was shown in our experiments through exogenous applications and endogenous level determination of the hormone in cotyledons of seedlings grown under special light conditions.</p><p>The application of ABA to cotyledons or shoot apices during the first half of a 24-h long inductive night inhibits flowering. The same compound applied towards the end of or after a 14-h long subinductive night increases the number of flower buds produced by these plants.</p><p>Exposing <em>P. nil</em> seedlings at the beginning of a 24-h long inductive night to far red light (FR) decreases the level of endogenous abscisic acid in cotyledons and leads to flower inhibition. However, a pulse of red light (R) reversing the inhibitory effect of far red light on the flowering of <em>P. nil</em> increases the ABA content.</p><p>The results obtained confirm previous observations that ABA may play a dual and an important role in the regulation of floral bud formation in <em>P. nil</em>. The flowering occurs when the level of endogenous abscisic acid is low at the beginning and is high toward the end of the inductive night.</p>

scholarly journals Rapid, sensitive, and validated UPLC/Q-TOF-MS method for quantitative determination of vasicine in Adhatoda vasica and its in vitro culture

2014 ◽  
Vol 10 (37) ◽  
pp. 198 ◽  
Author(s):  
Garg Madhukar ◽  
EnnusTajuddin Tamboli ◽  
Parveen Rabea ◽  
SH Ansari ◽  
MZ Abdin ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
In Vitro Culture ◽  
Adhatoda Vasica ◽  
Tof Ms

scholarly journals Endogenous Abscisic Acid and Dormancy of in Vitro Regenerated Lily Bulblets

1994 ◽  
Vol 8 (1) ◽  
pp. 30-35 ◽  
Author(s):  
A. Ivanova ◽  
M. Gerets ◽  
G. Jan De Klerk ◽  
H. Van Onckelen ◽  
A. Atanassov ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Endogenous Abscisic Acid

IN VITRO CULTURE OF TWO IRANIAN TABLE GRAPES AND DETERMINATION OF THE BEST CONDITIONS FOR THEIR MERISTEM CULTURE

2007 ◽  
pp. 325-332
Author(s):  
S. Kalatejari ◽  
A. Ebadi ◽  
Z. Zamani ◽  
R. Fatahi ◽  
M. Omidi
Keyword(s):  
In Vitro Culture ◽  
Meristem Culture ◽  
Table Grapes

Determination of erythrocyte susceptibility of Chinese sheep (Tan mutton breed) and French sheep (Vendéen breed) to Babesia sp. BQ1 (Lintan) by in vitro culture

2010 ◽  
Vol 170 (1-2) ◽  
pp. 37-43 ◽  
Author(s):  
Guiquan Guan ◽  
Emmanuelle Moreau ◽  
Nadine Brisseau ◽  
Jianxun Luo ◽  
Hong Yin ◽  
...  
Keyword(s):  
In Vitro Culture
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