Protein bodies in ray cells of Populus x canadensis Moench ‘robusta’

Planta ◽  
1988 ◽  
Vol 173 (1) ◽  
pp. 31-34 ◽  
Author(s):  
Jörg J. Sauter ◽  
Barbara van Cleve ◽  
Klaus Apel
Keyword(s):  
Protein Bodies ◽  
Ray Cells ◽  
Populus X Canadensis

Protein Storing Vacuoles in Ray Ceils of Willow Wood (Salix Caprea L.)

IAWA Journal ◽  
1988 ◽  
Vol 9 (1) ◽  
pp. 59-65 ◽  
Author(s):  
Jörg J. Sauter ◽  
Silvia Wellenkamp
Keyword(s):  
Dry Weight ◽  
Protein Bodies ◽  
Protein Determination ◽  
Thin Sections ◽  
Salix Caprea ◽  
Coomassie Blue ◽  
Ray Cells ◽  
Dormant Season ◽  
Protein Staining ◽  
Electron Microscopical

Light- and electron-microscopical investigations revealed protein bodies of c. 0.5 to 2.5 µm in diameter in the ray cells of willow wood. They consist of electron-dense aggregatesofvarious structural organisation which are enclosed in small-sized vacuole-like compartrnents. In semi-thin sections these aggregates showed positive protein staining with Ponçeau Red and Coomassie Blue, and enzymatic digestibility with pepsin. Because these protein bodies are found during the dormant season but not during summer, they are believed to be specific sites of protein storage in the ray cells of the wood. This is in accordance with the biochemical protein determination which yielded 6.4 to 8.4 µg mg-1 dry weight in late fall but only 1.2 to 2.0 µg mg-1 dry weight during summer.

Where is the prolamine located in rice endosperm?

1990 ◽  
Vol 48 (3) ◽  
pp. 696-697
Author(s):  
Hsin-Kan Wu ◽  
Mei-Chu Chung
Keyword(s):  
Storage Protein ◽  
Sodium Phosphate ◽  
Recent Experiment ◽  
Protein A ◽  
Uranyl Acetate ◽  
Protein Bodies ◽  
Lead Citrate ◽  
Thin Sections ◽  
Rice Endosperm ◽  
Ethanol Series

In one of our earlier papers (Wu et al. 1978), we suggested that glutelin is the major composition of the round storage protein bodies although they also contain relatively more prolamine than the angular one does. Immunochemical studies of Krishnan et al. (1986) later showed the presence of glutelin in the irregularly-shaped (angular) protein bodies while the prolamines were found in the round ones. Our recent experiment using protein A-gold technique found that prolamine is mainly deposited into the angular protein bodies.Small blocks (1 mm3) of 7 DAF (days after flowering) caryopsis of Orvza perennis were fixed with 3% paraformaldehyde and 3% glutaraldehyde in 0.1M sodium phosphate, pH7.4, dehydrated in a graded ethanol series and infiltrated with Spurr’s resin. Thin sections, after gold labeling, were stained with uranyl acetate and lead citrate. Rabbit antibodies were raised against purified prolamine. Protein A-gold sol complex was prepared based on the technique of Horisberger et al. (1977).

Influence of anatomy on the adhesion performance of four wood species

2020 ◽  
Vol 9 (4) ◽  
pp. e31942727
Author(s):  
João Gabriel Missia da Silva ◽  
Pedro Nicó de Medeiros ◽  
Denise Ransolin Soranso ◽  
Vinicius Peixoto Tinti ◽  
José Tarcísio da Silva Oliveira ◽  
...  
Keyword(s):  
Shear Strength ◽  
Specific Gravity ◽  
Wood Species ◽  
Tectona Grandis ◽  
Glue Line ◽  
Anatomical Characteristics ◽  
Ray Cells ◽  
Adhesion Performance ◽  
Adhesion Process

The aim of this study was to evaluate the influence of anatomical characteristics on the adhesion performance of Vatairea sp., Paulownia sp., Aspidosperma populifolium and Tectona grandis wood. Specimens for anatomical, physical and mechanical analyzes were produced from tangentially oriented boards. The treatments were joint glued from pieces of the same anatomical orientation (radial and tangential), evaluated for shear strength and glue line failure. The Vatairea sp wood had the highest specific gravity (0.74 g cm-3) and the Paulownia sp (0.34 g cm-3) wood was smaller. Aspidosperma populifolium species showed the highest shear strength in the glue line in the tangential and radial faces. The anatomical variables with higher influence on the wood adhesion process were pith ray cells and especially fibers that exhibit the greatest correlation with the shear strength of the glue line.

Thin-section, freeze-fracture, and energy dispersive x-ray analysis studies of the protein bodies of tomato seeds

1980 ◽  
Vol 58 (6) ◽  
pp. 699-711 ◽  
Author(s):  
Ernest Spitzer ◽  
John N. A. Lott
Keyword(s):  
Freeze Fracture ◽  
Protein Body ◽  
Cell Types ◽  
Energy Dispersive ◽  
Protein Bodies ◽  
Elemental Content ◽  
Tomato Seed ◽  
Endosperm Tissue ◽  
X Ray ◽  
Edx Analysis

Protein bodies of dry seeds of tomato (Lycopersicon esculentum) from radicle, hypocotyl, cotyledon, and endosperm tissue were extensively studied using thin-sectioning, freeze-fracturing and energy dispersive x-ray (EDX) analysis. Protein bodies varied in size, were oval to circular in section, and generally consisted of a proteinaceous matrix, globoid crystal, and protein crystalloid components. Size, shape, and arrangements of globoid crystals and protein crystalloids varied even within the same cell. Globoid crystals were generally oval to circular in section. They were always surrounded by a proteinaceous matrix. In a given protein body the number present ranged from a few to numerous. A protein body generally contained only one protein crystalloid. In section, protein crystalloids were irregular or angular in shape. They were composed of substructural particles which formed lattice planes. EDX analysis of tomato seed globoid crystals revealed the presence of P, K, and Mg in all cases, a fact that is consistent with globoid crystals being phytin-rich. Rarely, small amounts of calcium were found along with P, K, and Mg in globoid crystals of each of the tissue regions considered. The distribution pattern of cells with Ca containing globoid crystals was random. Small amounts of Fe and Mn were also found in the globoid crystals of protein bodies from certain cell types. These two elements, unlike calcium, were specific in terms of their distribution. Globoid crystals from the protodermal cells often contained Mn and Fe. The globoid crystals from provascular tissue of radicle, hypocotyl, and cotyledon regions often contained Fe while globoid crystals in the first layer of large cells surrounding these provascular areas always contained Fe. Results from EDX analysis of the proteinaceous material from the protein bodies are presented and discussed as are variations in elemental content due to different fixations.

scholarly journals Synthesis and Deposition of Zein in Protein Bodies of Maize Endosperm

1978 ◽  
Vol 62 (2) ◽  
pp. 256-263 ◽  
Author(s):  
Brian A. Larkins ◽  
William J. Hurkman
Keyword(s):  
Protein Bodies ◽  
Maize Endosperm

Nondispersive Protein Bodies in Sieve Elements: A Survey and Review of their Origin, Distribution and Taxonomic Significance

IAWA Journal ◽  
1991 ◽  
Vol 12 (2) ◽  
pp. 143-175 ◽  
Author(s):  
H.-Dietmar Behnke
Keyword(s):  
Electron Microscope ◽  
Protein Bodies ◽  
Evolutionary Trends ◽  
Taxonomic Significance ◽  
Additional Species ◽  
Sieve Elements ◽  
P Protein ◽  
Taxonomic Range ◽  
Characteristic Features

Nondispersive protein bodies present in the sieve elements in addition to dispersive P-protein are characteristic features of many woody dicotyledons; their origin may be nuclear or cytoplasmic. While nuclear nondispersive protein bodies are found in only two families, the Boraginaceae and Myristicaceae, bodies of cytoplasmic origin are present in 39 of the more than 350 families screened. These results were obtained from 228 dicotyledons studied with the electron microscope and data of additional species from the literature. The terminology, origin, form and distribution of nondispersive protein bodies are discussed. Their ultrastructural composition is described as being predominantly spindle-shaped, compound- spherical, rod-shaped and rosette-like. Based on the data accumulated from over 450 species (of about 3000 screened) it is evident that their taxonomic range is confined to a few dicotyledon superorders. Compound-spherical nondispersive protein bodies are characteristic of most of the Malvanae/Violanae; spindle-shaped forms are restricted to the Fabaceae (Rutanae). Rosanae-Proteanae-Myrtanae and the Magnolianae are the only other superorders that contain nondispersive protein bodies in several of their families. Evolutionary trends and possible taxonomic consequences implied in this distribution are discussed.

Sign in / Sign up

Export Citation Format

Share Document