Molecular characterization of the two genes SNQ and SFA that confer Hyperresistance to 4-nitroquinoline-N-oxide and formaldehyde in Saccharomyces cerevisiae

1989 ◽  
Vol 16 (2) ◽  
pp. 65-74 ◽  
Author(s):  
Patricia G�mpel-Klein ◽  
Michael Mack ◽  
Martin Brendel
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Molecular Characterization

scholarly journals Molecular characterization of Saccharomyces cerevisiae Δ3,Δ2-enoyl-CoA isomerase. Vol. 273 (1998) 33184–33191

2005 ◽  
Vol 280 (13) ◽  
pp. 13203
Author(s):  
Brian V. Geisbrecht ◽  
Dai Zhu ◽  
Kerstin Schulz ◽  
Katja Nau ◽  
James C. Morrell ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Molecular Characterization

scholarly journals Molecular Characterization of Saccharomyces cerevisiae TFIID

2002 ◽  
Vol 22 (16) ◽  
pp. 6000-6013 ◽  
Author(s):  
Steven L. Sanders ◽  
Krassimira A. Garbett ◽  
P. Anthony Weil
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Transcription Factor ◽  
Molecular Mass ◽  
Molecular Characterization ◽  
Biochemical Characterization ◽  
Gel Filtration ◽  
Calculated Molecular Mass ◽  
General Transcription Factor

ABSTRACT We previously defined Saccharomyces cerevisiae TFIID as a 15-subunit complex comprised of the TATA binding protein (TBP) and 14 distinct TBP-associated factors (TAFs). In this report we give a detailed biochemical characterization of this general transcription factor. We have shown that yeast TFIID efficiently mediates both basal and activator-dependent transcription in vitro and displays TATA box binding activity that is functionally distinct from that of TBP. Analyses of the stoichiometry of TFIID subunits indicated that several TAFs are present at more than 1 copy per TFIID complex. This conclusion was further supported by coimmunoprecipitation experiments with a systematic family of (pseudo)diploid yeast strains that expressed epitope-tagged and untagged alleles of the genes encoding TFIID subunits. Based on these data, we calculated a native molecular mass for monomeric TFIID. Purified TFIID behaved in a fashion consistent with this calculated molecular mass in both gel filtration and rate-zonal sedimentation experiments. Quite surprisingly, although the TAF subunits of TFIID cofractionated as a single complex, TBP did not comigrate with the TAFs during either gel filtration chromatography or rate-zonal sedimentation, suggesting that TBP has the ability to dynamically associate with the TFIID TAFs. The results of direct biochemical exchange experiments confirmed this hypothesis. Together, our results represent a concise molecular characterization of the general transcription factor TFIID from S. cerevisiae.

Physiological and molecular characterization of iron stress-resistant Saccharomyces cerevisiae mutant obtained by evolutionary engineering

2012 ◽  
Vol 29 ◽  
pp. S144
Author(s):  
B. Gülçin Balaban ◽  
Ülkü Yılmaz ◽  
Sema Gündüz ◽  
Süleyman Akman ◽  
Z. Petek Çakar
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Molecular Characterization ◽  
Evolutionary Engineering ◽  
Iron Stress

Evolutionary engineering and molecular characterization of a caffeine-resistant Saccharomyces cerevisiae strain

2019 ◽  
Vol 35 (12) ◽  
Author(s):  
Yusuf Sürmeli ◽  
Can Holyavkin ◽  
Alican Topaloğlu ◽  
Mevlüt Arslan ◽  
Halil İbrahim Kısakesen ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Molecular Characterization ◽  
Evolutionary Engineering
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