The frequency of P1 transduction of the genes of Escherichia coli as a function of chromosomal position: Preferential transduction of the origin of replication

1977 ◽  
Vol 155 (2) ◽  
pp. 197-202 ◽  
Author(s):  
Millicent Masters
Keyword(s):  
Escherichia Coli ◽  
Origin Of Replication ◽  
Chromosomal Position ◽  
P1 Transduction

Origin of Replication, oriC, of the Escherichia coli K12 Chromosome: Nucleotide Sequence

1979 ◽  
Vol 43 (0) ◽  
pp. 139-145 ◽  
Author(s):  
W. Messer ◽  
M. Meijer ◽  
H. E. N. Bergmans ◽  
F. G. Hansen ◽  
K. von Meyenburg ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Nucleotide Sequence ◽  
Origin Of Replication ◽  
Escherichia Coli K12

Origin of replication of Escherichia coli plasmid RSF 1030

1982 ◽  
Vol 187 (3) ◽  
pp. 375-383 ◽  
Author(s):  
Tapan Som ◽  
Jun-ichi Tomizawa
Keyword(s):  
Escherichia Coli ◽  
Origin Of Replication ◽  
Coli Plasmid

scholarly journals The Stringent Response Inhibits DNA Replication Initiation inE. coliby Modulating Supercoiling oforiC

mBio ◽  
2019 ◽  
Vol 10 (4) ◽  
Author(s):  
James A. Kraemer ◽  
Allen G. Sanderlin ◽  
Michael T. Laub
Keyword(s):  
Escherichia Coli ◽  
Dna Replication ◽  
Stringent Response ◽  
Replication Initiation ◽  
Initiation Factor ◽  
Cell Physiology ◽  
Origin Of Replication ◽  
Content Type ◽  
Dna Replication Initiation ◽  
Negative Supercoils

ABSTRACTThe stringent response enables bacteria to respond to a variety of environmental stresses, especially various forms of nutrient limitation. During the stringent response, the cell produces large quantities of the nucleotide alarmone ppGpp, which modulates many aspects of cell physiology, including reprogramming transcription, blocking protein translation, and inhibiting new rounds of DNA replication. The mechanism by which ppGpp inhibits DNA replication initiation inEscherichia coliremains unclear. Prior work suggested that ppGpp blocks new rounds of replication by inhibiting transcription of the essential initiation factordnaA, but we found that replication is still inhibited by ppGpp in cells ectopically producing DnaA. Instead, we provide evidence that a global reduction of transcription by ppGpp prevents replication initiation by modulating the supercoiling state of the origin of replication,oriC. Active transcription normally introduces negative supercoils intooriCto help promote replication initiation, so the accumulation of ppGpp reduces initiation potential atoriCby reducing transcription. We find that maintaining transcription nearoriC, either by expressing a ppGpp-blind RNA polymerase mutant or by inducing transcription from a ppGpp-insensitive promoter, can strongly bypass the inhibition of replication by ppGpp. Additionally, we show that increasing global negative supercoiling by inhibiting topoisomerase I or by deleting the nucleoid-associated protein geneseqAalso relieves inhibition. We propose a model, potentially conserved across proteobacteria, in which ppGpp indirectly creates an unfavorable energy landscape for initiation by limiting the introduction of negative supercoils intooriC.IMPORTANCETo survive bouts of starvation, cells must inhibit DNA replication. In bacteria, starvation triggers production of a signaling molecule called ppGpp (guanosine tetraphosphate) that helps reprogram cellular physiology, including inhibiting new rounds of DNA replication. While ppGpp has been known to block replication initiation inEscherichia colifor decades, the mechanism responsible was unknown. Early work suggested that ppGpp drives a decrease in levels of the replication initiator protein DnaA. However, we found that this decrease is not necessary to block replication initiation. Instead, we demonstrate that ppGpp leads to a change in DNA topology that prevents initiation. ppGpp is known to inhibit bulk transcription, which normally introduces negative supercoils into the chromosome, and negative supercoils near the origin of replication help drive its unwinding, leading to replication initiation. Thus, the accumulation of ppGpp prevents replication initiation by blocking the introduction of initiation-promoting negative supercoils. This mechanism is likely conserved throughout proteobacteria.

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