Development of rapid ammonium uptake during starvation of batch and chemostat cultures of the marine diatom Thalassiosira pseudonana

1984 ◽  
Vol 83 (1) ◽  
pp. 43-50 ◽  
Author(s):  
J. S. Parslow ◽  
P. J. Harrison ◽  
P. A. Thompson
Keyword(s):  
Thalassiosira Pseudonana ◽  
Marine Diatom ◽  
Ammonium Uptake ◽  
Chemostat Cultures

scholarly journals Distinct genome‐wide alternative polyadenylation during the response to silicon availability in the marine diatom Thalassiosira pseudonana

2019 ◽  
Vol 99 (1) ◽  
pp. 67-80 ◽  
Author(s):  
Haihui Fu ◽  
Peng Wang ◽  
Xiaohui Wu ◽  
Xiaoxuan Zhou ◽  
Guoli Ji ◽  
...  
Keyword(s):  
Alternative Polyadenylation ◽  
Thalassiosira Pseudonana ◽  
Marine Diatom ◽  
Genome Wide

Citrate as a possible iron-pooling substance in the marine diatom Thalassiosira pseudonana

2016 ◽  
Vol 163 (6) ◽  
Author(s):  
Yuki Imura ◽  
Keiko Sasai ◽  
Shin-ya Inui ◽  
Etsuro Yoshimura
Keyword(s):  
Thalassiosira Pseudonana ◽  
Marine Diatom

Rapid and precise genome editing in a marine diatom, Thalassiosira pseudonana by Cas9 nickase (D10A)

2020 ◽  
Vol 47 ◽  
pp. 101855
Author(s):  
Hermanus Nawaly ◽  
Yoshinori Tsuji ◽  
Yusuke Matsuda
Keyword(s):  
Genome Editing ◽  
Thalassiosira Pseudonana ◽  
Marine Diatom

scholarly journals A Genome-Scale Metabolic Model of Thalassiosira pseudonana CCMP 1335 for a Systems-Level Understanding of Its Metabolism and Biotechnological Potential

2020 ◽  
Vol 8 (9) ◽  
pp. 1396
Author(s):  
Ahmad Ahmad ◽  
Archana Tiwari ◽  
Shireesh Srivastava
Keyword(s):  
Carbon Fixation ◽  
Electron Flow ◽  
Single Gene ◽  
Metabolic Model ◽  
Thalassiosira Pseudonana ◽  
Marine Diatom ◽  
Omega 3 ◽  
Biotechnological Potential ◽  
A Genome ◽  
Genome Scale

Thalassiosira pseudonana is a transformable and biotechnologically promising model diatom with an ability to synthesise nutraceuticals such as fucoxanthin and store a significant amount of polyglucans and lipids including omega-3 fatty acids. While it was the first diatom to be sequenced, a systems-level analysis of its metabolism has not been done yet. This work presents first comprehensive, compartmentalized, and functional genome-scale metabolic model of the marine diatom Thalassiosira pseudonana CCMP 1335, which we have termed iThaps987. The model includes 987 genes, 2477 reactions, and 2456 metabolites. Comparison with the model of another diatom Phaeodactylum tricornutum revealed presence of 183 unique enzymes (belonging primarily to amino acid, carbohydrate, and lipid metabolism) in iThaps987. Model simulations showed a typical C3-type photosynthetic carbon fixation and suggested a preference of violaxanthin–diadinoxanthin pathway over violaxanthin–neoxanthin pathway for the production of fucoxanthin. Linear electron flow was found be active and cyclic electron flow was inactive under normal phototrophic conditions (unlike green algae and plants), validating the model predictions with previous reports. Investigation of the model for the potential of Thalassiosira pseudonana CCMP 1335 to produce other industrially useful compounds suggest iso-butanol as a foreign compound that can be synthesized by a single-gene addition. This work provides novel insights about the metabolism and potential of the organism and will be helpful to further investigate its metabolism and devise metabolic engineering strategies for the production of various compounds.

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