Nitrate reductase is not accumulated in chloroplast-ribosome-deficient mutants of higher plants

Planta ◽  
1986 ◽  
Vol 169 (2) ◽  
pp. 202-207 ◽  
Author(s):  
T. Borner ◽  
R. R. Mendel ◽  
J. Schiemann
Keyword(s):  
Nitrate Reductase ◽  
Higher Plants

scholarly journals Some Characteristics of Nitrate Reductase From Higher Plants

1968 ◽  
Vol 43 (6) ◽  
pp. 930-940 ◽  
Author(s):  
L. E. Schrader ◽  
G. L. Ritenour ◽  
G. L. Eilrich ◽  
R. H. Hageman
Keyword(s):  
Nitrate Reductase ◽  
Higher Plants

scholarly journals Nitrate Reductase and Soluble Cytochrome c Reductase(s) in Higher Plants

1978 ◽  
Vol 61 (5) ◽  
pp. 748-752 ◽  
Author(s):  
William Wallace ◽  
Christopher B. Johnson
Keyword(s):  
Cytochrome C ◽  
Nitrate Reductase ◽  
Higher Plants ◽  
Cytochrome C Reductase

scholarly journals ATIVIDADE DA ENZIMA NITRATO REDUTASE EM ARROZ DE TERRAS ALTAS SOB CONDIÇÕES DE ESTRESSE HÍDRICOS

Nativa ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 484-489
Author(s):  
Monique Carolina Nunes Fernandes ◽  
Flávia Barbosa Silva Botelho ◽  
Kamila Rezende Dázio de Souza ◽  
Gabrielle Carvalho Pereira ◽  
Camila Soares Cardoso da Silva ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Water Stress ◽  
Nitrate Reductase ◽  
Agronomic Traits ◽  
Higher Plants ◽  
Upland Rice ◽  
Incubation Solution ◽  
Crop Cycle ◽  
Rice Genotypes ◽  
Enzymatic Expression

A assimilação de nitrogênio é um processo vital que controla o crescimento e desenvolvimento da planta, garantindo bons níveis de produtividade de grãos. A enzima nitrato redutase (NR) catalisa o primeiro passo enzimático da assimilação de nitrogênio pelas plantas superiores por meio da redução do nitrato (NO3-) a nitrito (NO2-).  Assim, objetivou-se estudar a atividade da enzima redutase do nitrato em genótipos de arroz de terras altas e correlacioná-la com os demais caracteres agronômicos. O experimento foi conduzido em dois ambientes distintos, com e sem irrigação suplementar. As análises de atividade enzimática foram realizadas em laboratório. Foram avaliados 20 genótipos de um experimento de VCU na safra 2014/2015. O delineamento experimental foi em blocos casualizados com três repetições. Foi obtida a quantidade de nitrito liberado pelos tecidos vegetais na solução de incubação (µmoles NO2- gmf -1 h-1) em sete coletas realizadas aos 7, 14, 21, 28, 45, 75 e 100 dias após emergência (DAE), sempre no período da manhã, em todas as parcelas. Diante dos resultados, observou-se que a atividade da enzima NR, na cultura do arroz, é dependente do genótipo, do período de desenvolvimento vegetal e das condições ambientais, sendo de maior expressão no início do ciclo da cultura e em ambientes sem a ocorrência de estresse hídrico. A atividade da enzima RN não deve ser utilizada isoladamente para seleção indireta no caráter produtividade na cultura do arroz de terras altas, é necessário avaliar outras características que complementem à seleção.Palavras-chave: Oryza sativa; nitrogênio; melhoramento de plantas; expressão enzimática. ACTIVITY OF THE NITRATE REDUCTASE ENZYME IN UPLAND RICE UNDER WATER STRESS CONDITIONS  ABSTRACT:Nitrogen uptake is a vital process that controls plant growth and development, ensuring great grain yield levels. The enzyme nitrate reductase (NR) catalyzes the first enzymatic step of nitrogen uptake by higher plants by reducing nitrate (NO3-) to nitrite (NO2-). Thus, the objective was to study the activity of nitrate reductase enzyme in upland rice genotypes and to correlate it with other agronomic traits. The experiment was conducted in two distinct environments, with and without supplemental irrigation. The enzymatic activity assays were performed in the laboratory. Twenty genotypes of a VCU experiment in the 2014/2015 crop were evaluated. The experimental design was in randomized blocks with three replications. The amount of nitrite released by the plant tissues in the incubation solution (µmoles NO2-gmf -1 h-1) was obtained in seven collections performed at 7, 14, 21, 28, 45, 75 and 100 days after emergence (DAE). always in the mornings, in all installments. Given the results, it was observed that the activity of NR enzyme in rice culture is dependent on genotype, plant development period and environmental conditions, being more expressive at the beginning of the crop cycle and in environments without occurrence of water stress. The activity of the RN enzyme should not be used alone for indirect selection in the productivity character in the upland rice crop, it is necessary to evaluate other characteristics that complement the selection.Keywords: Oryza sativa; nitrogen; plant breeding; enzymatic expression.

scholarly journals Characteristics of Nicotiana tabacum nitrate reductase protein produced in Saccharomyces cerevisiae

1991 ◽  
Vol 278 (2) ◽  
pp. 393-397 ◽  
Author(s):  
H N Truong ◽  
C Meyer ◽  
F Daniel-Vedele
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Nicotiana Tabacum ◽  
Cytochrome C ◽  
Nitrate Reductase ◽  
Higher Plants ◽  
Reductase Activity ◽  
Molybdenum Cofactor ◽  
Biochemical Data ◽  
Native Form ◽  
Nr Activity

Tobacco nitrate reductase (NR) produced in yeast retains cytochrome c reductase activity, but not NR activity. Biochemical data suggest that the haem and FAD domains are functional, and that the molybdenum cofactor (MoCo) domain is inactive owing to the absence of MoCo in yeast. The native form of the produced NR is dimeric. Thus MoCo is not involved in NR dimerization in higher plants, contrary to current assumptions.

Nitrite activation of nitrate reductase in higher plants

Planta ◽  
1978 ◽  
Vol 138 (3) ◽  
pp. 205-209 ◽  
Author(s):  
D. Kaplan ◽  
A. M. Mayer ◽  
S. H. Lips
Keyword(s):  
Nitrate Reductase ◽  
Higher Plants
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