The common mussel Mytilus edulis as an indicator of trace metals in Scandinavian waters. II. lead, iron and manganese

1978 ◽  
Vol 46 (2) ◽  
pp. 147-156 ◽  
Author(s):  
D. J. H. Phillips
Author(s):  
Anthony A. Paparo ◽  
Judith A. Murphy

The purpose of this study was to localize the red neuronal pigment in Mytilus edulis and examine its role in the control of lateral ciliary activity in the gill. The visceral ganglia (Vg) in the central nervous system show an over al red pigmentation. Most red pigments examined in squash preps and cryostat sec tions were localized in the neuronal cell bodies and proximal axon regions. Unstained cryostat sections showed highly localized patches of this pigment scattered throughout the cells in the form of dense granular masses about 5-7 um in diameter, with the individual granules ranging from 0.6-1.3 um in diame ter. Tissue stained with Gomori's method for Fe showed bright blue granular masses of about the same size and structure as previously seen in unstained cryostat sections.Thick section microanalysis (Fig.l) confirmed both the localization and presence of Fe in the nerve cell. These nerve cells of the Vg share with other pigmented photosensitive cells the common cytostructural feature of localization of absorbing molecules in intracellular organelles where they are tightly ordered in fine substructures.


1960 ◽  
Vol 32 (1) ◽  
pp. 223-228
Author(s):  
Osmo Mäkitie

The experiments show that under these conditions the common trace nutrients, cobalt, copper, manganese, molybdenum and zinc are sufficiently completely extracted as chelates by shaking the soil extract with oxine-chloroform solution. The hydrogen ion concentration of the extract and the concentration of oxine in chloroform have decisive effects on the extractability. Using the reported and discussed procedure it is possible to separate the common trace metals from the major soil extract constituents, especially for spectrographic analysis.


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