A chimeric gene encoding the methionine-rich 2S albumin of the Brazil nut (Bertlrolletia excelsa H.B.K.) is stably expressed and inherited in transgenic grain legumes

1994 ◽  
Vol 242 (2) ◽  
pp. 226-236 ◽  
Author(s):  
Isolde Saalbach ◽  
Thomas Pickardt ◽  
Frank Machemehl ◽  
Gerhard Saalbach ◽  
Otto Schieder ◽  
...  
Keyword(s):  
Grain Legumes ◽  
Chimeric Gene ◽  
Brazil Nut ◽  
Gene Encoding ◽  
2S Albumin

Stability of the major allergen Brazil nut 2S albumin (Ber e 1) to physiologically relevant in vitro gastrointestinal digestion

FEBS Journal ◽  
2004 ◽  
Vol 272 (2) ◽  
pp. 341-352 ◽  
Author(s):  
F. Javier Moreno ◽  
Fred A. Mellon ◽  
Martin S. J. Wickham ◽  
Andrew R. Bottrill ◽  
E. N. Clare Mills
Keyword(s):  
Major Allergen ◽  
Brazil Nut ◽  
Gastrointestinal Digestion ◽  
2S Albumin ◽  
In Vitro Gastrointestinal Digestion

Reversible Denaturation of Brazil Nut 2S Albumin (Ber e1) and Implication of Structural Destabilization on Digestion by Pepsin

2005 ◽  
Vol 53 (1) ◽  
pp. 123-131 ◽  
Author(s):  
Stef J. Koppelman ◽  
Willem F. Nieuwenhuizen ◽  
Marco Gaspari ◽  
Leon M. J. Knippels ◽  
André H. Penninks ◽  
...  
Keyword(s):  
Brazil Nut ◽  
2S Albumin

scholarly journals The protein structure determines the sensitizing capacity of Brazil nut 2S albumin (Ber e1) in a rat food allergy model

2013 ◽  
Vol 3 (1) ◽  
Author(s):  
Jolanda HM Van Bilsen ◽  
Léon MJ Knippels ◽  
André H Penninks ◽  
Willem F Nieuwenhuizen ◽  
Harmen HJ De Jongh ◽  
...  
Keyword(s):  
Food Allergy ◽  
Protein Structure ◽  
Brazil Nut ◽  
2S Albumin

scholarly journals Structure and expression of the human gene encoding major heat shock protein HSP70.

1985 ◽  
Vol 5 (2) ◽  
pp. 330-341 ◽  
Author(s):  
B Wu ◽  
C Hunt ◽  
R Morimoto
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Genomic Library ◽  
Human Gene ◽  
Chimeric Gene ◽  
Hsp70 Gene ◽  
Gene Encoding ◽  
Hsp70 Mrna ◽  
Flanking Sequences ◽  
Heat Shock Protein Hsp70

We have cloned a human gene encoding the 70,000-dalton heat shock protein (HSP70) from a human genomic library, using the Drosophila HSP70 gene as a heterologous hybridization probe. The human recombinant clone hybridized to a 2.6-kilobase polyadenylated mRNA from HeLa cells exposed to 43 degrees C for 2 h. The 2.6-kilobase mRNA was shown to direct the translation in vitro of a 70,000-dalton protein similar in electrophoretic mobility to the HSP70 synthesized in vivo. From the analysis of S1 nuclease-resistant mRNA-DNA hybrids, the HSP70 gene appears to be transcribed as an uninterrupted mRNA of 2.3 kilobases. We show that the cloned HSP70 gene contains the sequences necessary for heat shock-induced expression by two criteria. First, hamster cells transfected with a subclone containing the HSP70 gene and flanking sequences synthesized a HSP70-like protein upon heat shock. Second, human cells transfected with a chimeric gene containing the 5' flanking sequences of the HSP70 gene and the coding sequences of the bacterial chloramphenicol acetyltransferase gene transcribed the chimeric gene upon heat shock. We show that the HSP70 mRNA transcribed in an adenovirus 5 transformed human cell line (293 cells) is identical to the HSP70 mRNA induced by heat shock.

Seed specific expression of the 2S albumin gene from Brazil nut (Bertholletia excelsa) in transgenicVicia narbonensis

1995 ◽  
Vol 1 (3) ◽  
pp. 295-301 ◽  
Author(s):  
Thomas Pickardt ◽  
Isolde Saalbach ◽  
David Waddell ◽  
Martin Meixner ◽  
Klaus Müntz ◽  
...  
Keyword(s):  
Brazil Nut ◽  
2S Albumin ◽  
Specific Expression ◽  
Bertholletia Excelsa ◽  
Albumin Gene ◽  
Seed Specific Expression
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