Mitoses in thermodormant lettuce seeds with reference to histological location, localized expansion, and seed storage

Planta ◽  
1966 ◽  
Vol 71 (2) ◽  
pp. 160-170 ◽  
Author(s):  
Donald E. Foard ◽  
Alan H. Haber
Keyword(s):  
Seed Storage ◽  
Lettuce Seeds ◽  
Histological Location

scholarly journals Primed Lettuce Seeds Exhibit Increased Sensitivity to Moisture Content During Controlled Deterioration

HortScience ◽  
2007 ◽  
Vol 42 (6) ◽  
pp. 1436-1439 ◽  
Author(s):  
H.J. Hill ◽  
Jesse D. Cunningham ◽  
Kent J. Bradford ◽  
A.G. Taylor
Keyword(s):  
Moisture Content ◽  
Elevated Temperatures ◽  
Seed Viability ◽  
Seed Storage ◽  
Seed Priming ◽  
Storage Conditions ◽  
Seed Moisture Content ◽  
Moisture Contents ◽  
Lettuce Seeds ◽  
Rate Of Decline

The Ellis-Roberts seed viability equation is used to predict seed survival after storage at specified temperatures and moisture contents. Seed priming, which can break dormancy and accelerate germination, can also reduce seed storage life. Because primed seeds were not used in developing the Ellis-Roberts equation, the reciprocal nature of specific seed moisture content (MC, fresh weight basis) and temperatures that applies to nonprimed lettuce (Lactuca sativa L.) seeds may not apply to primed seeds. To determine how priming affects lettuce seeds in relation to the viability equation, an experiment was conducted using two cultivars, ‘Big Ben’ and ‘Parris Island Cos’. Seeds primed in polyethylene glycol 8000 (–1.45 MPa, 24 h at 15 °C) and nonprimed seeds were first adjusted to 6% and 9% moisture contents and then stored at 48 and 38 °C for up to 30 days, respectively. These storage conditions (6% MC and 48 °C; 9% MC and 38 °C) were predicted by the viability equation to result in equal longevities. Subsequent viability assays at 20 °C revealed that nonprimed seeds in both storage environments exhibited similar losses in viability over time, thus validating the Ellis-Roberts equation and the use of these conditions to apply different but equal aging stress. Primed seeds of both cultivars deteriorated faster than nonprimed seeds as expected. However, primed seeds did exhibit different rates of deterioration between the storage environments. Primed seeds stored at 9% MC and 38 °C deteriorated faster than primed seeds stored at 6% MC and 48 °C. The rate of decline in probit viability percentage was three times greater in primed ‘Big Ben’ seeds stored at 9% MC and 38 °C than for those stored at 6% MC and 48 °C (–1.34 versus –0.26 probits per day, respectively). ‘Parris Island Cos’ seeds stored at 9% MC and 38 °C had twice the rate of deterioration that those stored at 6% MC and 48 °C (–1.19 and –0.49 probits per day, respectively). The results indicate that primed lettuce seeds were more sensitive to the adverse effects of higher seed MC than were nonprimed seeds during storage at elevated temperatures.

Molecular mobility in the cytoplasm of lettuce radicles correlates with longevity

2000 ◽  
Vol 10 (3) ◽  
pp. 285-292 ◽  
Author(s):  
Julia Buitink ◽  
Folkert A. Hoekstra ◽  
Marcus A. Hemminga
Keyword(s):  
Linear Relationship ◽  
Molecular Mobility ◽  
Rotational Motion ◽  
Storage Stability ◽  
Electron Paramagnetic Resonance Spectroscopy ◽  
Seed Storage ◽  
Seed Longevity ◽  
Resonance Spectroscopy ◽  
Survival Times ◽  
Lettuce Seeds

AbstractMolecular mobility is hypothesised to be a key factor influencing storage stability of seeds because it may control the rate of deteriorative reactions responsible for reduced shelf life. The relationship between the longevity of lettuce seeds and the molecular mobility was investigated in the cytoplasm of lettuce radicles. Longevity of lettuce seeds was predicted using the viability equation of Ellis and Roberts, and the molecular mobility was determined by saturation transfer electron paramagnetic resonance spectroscopy measurements of rotational motion of a polar spin probe inserted into the cytoplasm. Increasing the temperature resulted in faster rotational motion and shorter longevity. There was a linear relationship between the logarithms of rotational motion and estimated seed longevity for temperatures above 5°C. Below 5°C, there was a deviation from linearity. Based on the hypothesis that the molecular mobility in the cytoplasm determines the rate of detrimental reactions, seed longevity at sub-zero temperatures was predicted by extrapolation of the rotational motion using the linear relationship. Predictions of longevity at sub-zero temperatures based on rotational motion indicated longer survival times than those based on the viability equation. A kinetic approach to ageing using molecular mobility measurements is expected to improve our understanding of seed storage stability and might eventually lead to realistic predictions of longevity at low temperatures.

Barley seed storage under controlled conditions

2019 ◽  
pp. 140-150 ◽  
Author(s):  
O. A. Zadorozhna ◽  
T. P. Shyianova ◽  
M.Yu. Skorokhodov
Keyword(s):  
Hordeum Vulgare ◽  
Moisture Content ◽  
Spring Barley ◽  
Seed Storage ◽  
Growing Season ◽  
Storage Conditions ◽  
Meteorological Conditions ◽  
Hordeum Vulgare L ◽  
The Relationship ◽  
And Storage

Seed longevity of 76 spring barley gene pool samples (Hordeum vulgare L. subsp. distichon, convar. distichon: 56 nutans Schubl., two deficience (Steud.) Koern., two erectum Rode ex Shuebl., two medicum Koern.; convar. nudum (L.) A.Trof.: one nudum L. та subsp. vulgare: convar. vulgare: nine pallidum Ser., three rikotense Regel.; convar. coeleste (L.) A.Trof.: one coeleste (L.) A.Trof.) from 26 countries, 11 years and four places of reproduction was analyzed. Seeds with 5–8% moisture content were stored in chamber with unregulated and 4oC temperature. The possibility of seed storage under these conditions for at least 10 years without significant changes in germination has been established. The importance of meteorological conditions in the formation and ripening of seeds for their longevity is confirmed. The relationship between the decrease of barley seeds longevity and storage conditions, amount of rainfall, temperature regime during the growing season of plants is discussed.

Storage of primed pelleted sugar beet seed with minimal loss of seed vigour and active ingredients

2019 ◽  
pp. 89-92
Author(s):  
Martijn van Overveld ◽  
Martijn Leijdekkers ◽  
Noud van Swaaij
Keyword(s):  
Sugar Beet ◽  
Storage Temperature ◽  
Seed Storage ◽  
Cold Test ◽  
Test Plant ◽  
Active Ingredients ◽  
Seed Vigour ◽  
Plastic Bag ◽  
Commercial Sugar ◽  
Storage Methods

Different seed storage methods, varying in storage temperature, moisture and/or oxygen content, were applied to commercial sugar beet seed lots from four breeding companies. After storage for 10–11 months, germination of the seed was tested in the laboratory (cold test, 10°C). In addition, the contents of active ingredients (fungicides and insecticide) were analyzed and compared with the initial contents before storage. Based on these results, a selection of the most promising storage methods was made to test plant emergence in a field experiment. This research was performed in 2015/16 and in 2016/17. In both years, two storage treatments outperformed the others: these were storage in a closed jar with the addition of moisture absorber (i.e. silica gel) at room temperature and storage at –18°C in a closed plastic bag. Using these two storage methods, seed vigour and contents of active ingredients were comparable to those in seed that had not been stored for one year. Based on the results from this study, the advice to growers for a successful storage of residual sugar beet seed was adjusted in 2017, after including some practical guidelines and considerations.

A GROWTH-INHIBITING SUBSTANCE IN LETTUCE SEEDS

Science ◽  
1935 ◽  
Vol 81 (2096) ◽  
pp. 236-236 ◽  
Author(s):  
A. L. Shuck
Keyword(s):  
Lettuce Seeds ◽  
Growth Inhibiting
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