Protein synthesis of binucleate and trinucleate pollen and its relationship to tube emergence and growth

Planta ◽  
1979 ◽  
Vol 146 (5) ◽  
pp. 559-566 ◽  
Author(s):  
Folkert A. Hoekstra ◽  
Johan Bruinsma
Keyword(s):  
Protein Synthesis ◽  
Tube Emergence ◽  
Trinucleate Pollen

The germination of basidiospores from commercial and wild-collected isolates of Agaricus bisporus (= A. brunnescens)

1989 ◽  
Vol 35 (4) ◽  
pp. 492-498 ◽  
Author(s):  
Paul A. Horgen ◽  
Karl F. Kokurewicz ◽  
James B. Anderson
Keyword(s):  
Protein Synthesis ◽  
Spore Germination ◽  
Agaricus Bisporus ◽  
Germ Tube ◽  
Actinomycin D ◽  
Culture Media ◽  
Tube Emergence ◽  
Standard Set ◽  
Group Ii

A standard set of conditions yielding highly reproducible germination kinetics for basidiospores of the commercial mushroom Agaricus bisporus is described. Spores of three commercial, one wild-collected, and one intraspecies hybrid were examined. The effects of presence or absence of growing mycelium, temperature, culture media, and spore age are described. Cycloheximide completely blocked basidiospore germination and the incorporation of [3H]leucine into protein, whereas actinomycin D had no effect on germ tube emergence. Furthermore, [3H]uridine is not incorporated into spores before or during germination, neither in the presence nor absence of actinomycin D. Two types of basidiospore dormancy exist for A. bisporus: (i) exogenously dormant spores and (ii) constitutively dormant spores, both of the group II type in which protein synthesis precedes RNA synthesis.Key words: spore, germination, Agaricus, basidiomycete.

Homochirality as the Signature of Extra-Terrestrial Life

1997 ◽  
Vol 161 ◽  
pp. 505-510
Author(s):  
Alexandra J. MacDermott ◽  
Laurence D. Barron ◽  
Andrè Brack ◽  
Thomas Buhse ◽  
John R. Cronin ◽  
...  
Keyword(s):  
Amino Acids ◽  
Protein Synthesis ◽  
Optical Rotation ◽  
Physical Origin ◽  
Natural Choice ◽  
Rosetta Mission ◽  
Terrestrial Life ◽  
Subsurface Layers ◽  
Solar System Bodies ◽  
Origin Of Homochirality

AbstractThe most characteristic hallmark of life is its homochirality: all biomolecules are usually of one hand, e.g. on Earth life uses only L-amino acids for protein synthesis and not their D mirror images. We therefore suggest that a search for extra-terrestrial life can be approached as a Search for Extra- Terrestrial Homochirality (SETH). The natural choice for a SETH instrument is optical rotation, and we describe a novel miniaturized space polarimeter, called the SETH Cigar, which could be used to detect optical rotation as the homochiral signature of life on other planets. Moving parts are avoided by replacing the normal rotating polarizer by multiple fixed polarizers at different angles as in the eye of the bee. We believe that homochirality may be found in the subsurface layers on Mars as a relic of extinct life, and on other solar system bodies as a sign of advanced pre-biotic chemistry. We discuss the chiral GC-MS planned for the Roland lander of the Rosetta mission to a comet and conclude with theories of the physical origin of homochirality.

Freeze-substituted fungal cells of Nectria haematococca: A comparison of the macroconidial walls of the adhesion-competent wild type with an adhesion-reduced mutant

1990 ◽  
Vol 48 (3) ◽  
pp. 688-689
Author(s):  
Thecan Caesar-Ton That ◽  
Lynn Epstein
Keyword(s):  
Freeze Substitution ◽  
Uranyl Acetate ◽  
Wild Type ◽  
Nectria Haematococca ◽  
Fruit Extract ◽  
Dialysis Tubing ◽  
Tube Emergence ◽  
Contrast Microscopy ◽  
In The Wild ◽  
Freeze Damage

Nectria haematococca mating population I (anamorph, Fusarium solani) macroconidia attach to its host (squash) and non-host surfaces prior to germ tube emergence. The macroconidia become adhesive after a brief period of protein synthesis. Recently, Hickman et al. (1989) isolated N. haematococca adhesion-reduced mutants. Using freeze substitution, we compared the development of the macroconidial wall in the wild type in comparison to one of the mutants, LEI.Macroconidia were harvested at 1C, washed by centrifugation, resuspended in a dilute zucchini fruit extract and incubated from 0 - 5 h. During the incubation period, wild type macroconidia attached to uncoated dialysis tubing. Mutant macroconidia did not attach and were collected on poly-L-lysine coated dialysis tubing just prior to freezing. Conidia on the tubing were frozen in liquid propane at 191 - 193C, substituted in acetone with 2% OsO4 and 0.05% uranyl acetate, washed with acetone, and flat-embedded in Epon-Araldite. Using phase contrast microscopy at 1000X, cells without freeze damage were selected, remounted, sectioned and post-stained sequentially with 1% Ba(MnO4)2 2% uranyl acetate and Reynold’s lead citrate. At least 30 cells/treatment were examined.

Lymphocyte activity and protein synthesis

2001 ◽  
Vol 101 (6) ◽  
pp. 591
Author(s):  
DEREK C. MACALLAN
Keyword(s):  
Protein Synthesis ◽  
Lymphocyte Activity

Protein synthesis in mitochondria purified from roots, leaves and flowers of sugar beet

1991 ◽  
Vol 83 (1) ◽  
pp. 7-16 ◽  
Author(s):  
Christina Lind ◽  
Christer Hallden ◽  
Ian M. Moller
Keyword(s):  
Protein Synthesis ◽  
Sugar Beet

Respiratory energy requirements and rate of protein turnover in vivo determined by the use of an inhibitor of protein synthesis and a probe to assess its effect

1994 ◽  
Vol 92 (4) ◽  
pp. 585-594 ◽  
Author(s):  
T. J. Bouma ◽  
R. De Visser ◽  
J. H. J. A. Janssen ◽  
M. J. De Kock ◽  
P H. Van Leeuwen ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Protein Turnover ◽  
Energy Requirements ◽  
Inhibitor Of Protein Synthesis

Altered protein synthesis during in situ oat leaf senescence

1990 ◽  
Vol 80 (4) ◽  
pp. 619-623 ◽  
Author(s):  
Louise Lalonde ◽  
Rajinder S. Dhindsa
Keyword(s):  
Protein Synthesis ◽  
Leaf Senescence ◽  
Altered Protein

Age of potato seed-tubers influences protein synthesis during sprouting

1993 ◽  
Vol 89 (2) ◽  
pp. 262-270 ◽  
Author(s):  
G. N. Mohan Kumar ◽  
N. Richard Knowles
Keyword(s):  
Protein Synthesis ◽  
Potato Seed ◽  
Seed Tubers
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