B-L antigens (Class II) of the chicken major histocompatibility complex control T-B cell interaction

1984 ◽  
Vol 19 (2) ◽  
pp. 131-140 ◽  
Author(s):  
Olli Vainio ◽  
Claus Koch ◽  
Auli Toivanen
Keyword(s):  
Major Histocompatibility Complex ◽  
B Cell ◽  
Cell Interaction ◽  
Class Ii ◽  
Major Histocompatibility ◽  
Complex Control ◽  
Histocompatibility Complex ◽  
Chicken Major Histocompatibility Complex

scholarly journals Complex architecture of major histocompatibility complex class II promoters: reiterated motifs and conserved protein-protein interactions.

1996 ◽  
Vol 16 (9) ◽  
pp. 4683-4690 ◽  
Author(s):  
N Jabrane-Ferrat ◽  
J D Fontes ◽  
J M Boss ◽  
B M Peterlin
Keyword(s):  
Major Histocompatibility Complex ◽  
Major Histocompatibility Complex Class ◽  
B Cell ◽  
Interferon Gamma ◽  
Class Ii ◽  
Inducible Expression ◽  
Complex Class ◽  
Major Histocompatibility ◽  
Flanking Sequence ◽  
Histocompatibility Complex

The S box (also known as at the H, W, or Z box) is the 5'-most element of the conserved upstream sequences in promoters of major histocompatibility complex class II genes. It is important for their B-cell-specific and interferon gamma-inducible expression. In this study, we demonstrate that the S box represents a duplication of the downstream X box. First, RFX, which is composed of the RFX5-p36 heterodimer that binds to the X box, also binds to the S box and its 5'-flanking sequence. Second, NF-Y, which binds to the Y box and increases interactions between RFX and the X box, also increases the binding of RFX to the S box. Third, RFXs bound to S and X boxes interact with each other in a spatially constrained manner. Finally, we confirmed these protein-protein and protein-DNA interactions by expressing a hybrid RFX5-VP16 protein in cells. We conclude that RFX binds to S and X boxes and that complex interactions between RFX and NF-Y direct B-cell-specific and interferon gamma-inducible expression or major histocompatibility complex class II genes.

B-cell control region at the 5' end of a major histocompatibility complex class II gene: sequences and factors

1988 ◽  
Vol 8 (10) ◽  
pp. 3975-3987
Author(s):  
A Dorn ◽  
H J Fehling ◽  
W Koch ◽  
M Le Meur ◽  
P Gerlinger ◽  
...  
Keyword(s):  
Major Histocompatibility Complex ◽  
B Cells ◽  
Major Histocompatibility Complex Class ◽  
B Cell ◽  
Simian Virus 40 ◽  
Class Ii ◽  
Immunoglobulin Gene ◽  
Complex Class ◽  
Major Histocompatibility ◽  
Histocompatibility Complex

Transcription of major histocompatibility complex class II genes is elaborately regulated. Mouse class II genes are transcribed primarily in B cells, peripheral macrophages and interdigitating cells, and thymic cortical and medullary cells. In this study, we began to identify the DNA sequences and protein factors that control expression of a class II gene in B cells, addressing in particular how closely they resemble those that regulate immunoglobulin gene expression. We describe a region upstream of the E alpha gene that is crucial for its transcription in the B cells of transgenic mice but is less important in cultured B-cell lines. The sequence of this region reveals several familiar motifs, including a second X-Y pair reminiscent of that residing in the promoter-proximal region of all class II genes, a B motif strikingly homologous to that associated with the immunoglobulin kappa gene enhancer, several Ephrussi motifs, and a Pu box-like sequence very similar to that implicated in simian virus 40 and lymphotrophic papovavirus expression in B cells. Careful study of the proteins that bind specifically to these different motifs prompts us to suggest that major histocompatibility complex class II and immunoglobulin genes rely on quite different factors to achieve B-cell-specific expression.

scholarly journals Major histocompatibility complex class II expression distinguishes two distinct B cell developmental pathways during ontogeny.

1994 ◽  
Vol 180 (2) ◽  
pp. 507-516 ◽  
Author(s):  
K P Lam ◽  
A M Stall
Keyword(s):  
Major Histocompatibility Complex ◽  
B Cells ◽  
B Cell ◽  
Fetal Liver ◽  
Class Ii ◽  
Cell Development ◽  
B Cell Development ◽  
Developmental Pathways ◽  
Major Histocompatibility ◽  
Histocompatibility Complex

All mature B cells coexpress major histocompatibility complex (MHC) class II molecules, I-A and I-E, which are restriction elements required for antigen presentation to CD4+ T cells. However, the expression of class II during the early stages of B cell development has been unclear. We demonstrate here that there is a difference in the expression of class II during murine B cell development in the fetal liver and adult bone marrow (BM). These differences define two distinct B cell developmental pathways. The Fetal-type (FT) pathway is characterized by pre-B and immature IgM+ B cells generated in the fetal liver which initially lack all class II expression. In contrast, the Adult-type (AT) pathway is typified by B cells developing in the adult BM which express class II molecules from the pre-B cell stage. In vitro stromal cell cultures of sorted fetal liver and adult BM pro-B cells indicated that the difference in I-A expression during B cell development is intrinsic to the progenitors. In addition, we show that FT B cell development is not restricted to the fetal liver but occurs in the peritoneal cavities, spleens, liver, and BM of young mice up to at least 1 mo of age. The AT B cell development begins to emerge after birth but is, however, restricted to the BM environment. These findings indicate that there are two distinct B cell developmental pathways during ontogeny, each of which could contribute differentially to the immune repertoire and thus the functions of B cell subsets and lineages.

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