Charakterisierung der Microbodies aus Spadix-Appendices von Arum maculatum L. und Sauromatum guttatum Schott

Planta ◽  
1971 ◽  
Vol 96 (4) ◽  
pp. 326-338 ◽  
Author(s):  
Christel Berger ◽  
Bernt Gerhardt
Keyword(s):  
Arum Maculatum ◽  
Sauromatum Guttatum

scholarly journals Pyruvate transport by thermogenic-tissue mitochondria

1987 ◽  
Vol 247 (2) ◽  
pp. 441-447 ◽  
Author(s):  
M O Proudlove ◽  
R B Beechey ◽  
A L Moore
Keyword(s):  
Alternative Oxidase ◽  
Competitive Inhibition ◽  
Feedback Inhibition ◽  
Total Activity ◽  
Mitochondrial Inner Membrane ◽  
Pyruvate Oxidation ◽  
Cytochrome Pathway ◽  
Arum Maculatum ◽  
Long Chain ◽  
Sauromatum Guttatum

1. Mitochondria isolated from the thermogenic spadices of Arum maculatum and Sauromatum guttatum plants oxidized external NADH, succinate, citrate, malate, 2-oxoglutarate and pyruvate without the need to add exogenous cofactors. 2. Oxidation of substrates was virtually all via the alternative oxidase, the cytochrome pathway constituting only 10-20% of the total activity, depending on the stage of spadix development. 3. During later stages of spadix development, pyruvate oxidation was enhanced by the addition of aspartate. This was caused by acetyl-CoA condensing with oxaloacetate, produced from pyruvate/aspartate transamination, and so decreasing feedback inhibition of pyruvate dehydrogenase. 4. Pyruvate oxidation was inhibited by the long-chain acid maleimides AM5-11, but not by those with shorter polymethylene side groups, AM1-4. 5. The alpha-cyanocinnamate derivatives UK5099 [alpha-cyano-beta-(1-phenylindol-3-yl)acrylate] and CHCA [alpha-cyano-4-hydroxycinnamate] inhibited pyruvate-dependent O2 consumption and the carrier-mediated uptake of pyruvate across the mitochondrial inner membrane. Characteristics of non-competitive inhibition were observed for CHCA, whereas for UK5099 the results were more complex, suggesting a very low rate of dissociation of the inhibitor-carrier complex. 6. A comparison of the values of Vmax. and Km for oxidation and transport suggested that it was the latter which controls the overall rate of pyruvate oxidation by mitochondria from both tissues.

Hormonal control of the respiratory climacteric in Sauromatum guttatum (Araceae)

1971 ◽  
Vol 49 (8) ◽  
pp. 1373-1377 ◽  
Author(s):  
Richard G. Buggeln ◽  
Bastiaan J. D. Meeuse
Keyword(s):  
Staminate Flower ◽  
Hormonal Control ◽  
Terminal Portion ◽  
Flower Buds ◽  
Direct Addition ◽  
Controlled Conditions ◽  
Arum Maculatum ◽  
Time Required ◽  
Sauromatum Guttatum

A respiratory climacteric (RC) occurs in the appendix (terminal portion of the spadix) of the inflorescence of Sauromatum guttatum Schott during blooming. We have devised purification techniques and a bioassay for calorigen, the compound (synthesized in the staminate flower buds) which triggers the RC in the Sauromatum appendix.Inflorescences were induced to bloom under controlled conditions to determine (1) the time required for calorigen to be released from the staminate flowers and to accumulate in the appendix, and (2) the time required for calorigen to trigger the RC in the appendix. The latter results were confirmed by the direct addition of calorigen to appendix sections in a bioassay.A calorigen-like substance was extracted from the staminate flowers of Arum maculatum, A. italicum, A. dioscoridis, and Dracunculus vulgaris.

scholarly journals Characterization of cuckoo-pint (Arum maculatum) mitochondrial adenosine triphosphatases

1986 ◽  
Vol 233 (3) ◽  
pp. 839-844 ◽  
Author(s):  
P P J Dunn ◽  
A R Slabas ◽  
A L Moore
Keyword(s):  
Adenosine Triphosphatase ◽  
Kinetic Properties ◽  
Ph Profile ◽  
Enzyme Preparations ◽  
Adenosine Triphosphatases ◽  
Membrane Bound ◽  
Cold Stability ◽  
Arum Maculatum ◽  
Guanosine Triphosphatase ◽  
Soluble Enzymes

The catalytic properties of cuckoo-pint (Arum maculatum) mitochondrial adenosine triphosphatase have been analysed. The pH profile, effect of inhibitors, cold-stability and substrate specificity are characteristic of mitochondrial adenosine triphosphatases, although a high guanosine triphosphatase activity does appear to be restricted to plant mitochondrial adenosine triphosphatases. The kinetic properties of nucleoside 5′-triphosphate hydrolysis by membrane-bound and soluble enzymes have been studied by means of double-reciprocal plots. These plots were linear in the absence of an activating anion, which may indicate that the catalytic and/or regulatory mechanism of Arum maculatum adenosine triphosphatase is different from that of other enzyme preparations. It is suggested that the differences in subunit composition of plant and mammalian adenosine triphosphatases reported previously [Dunn, Slabas & Moore (1985) Biochem. J. 225, 821-824] are structurally, rather than functionally, significant.

Arum italicum subsp. albispathum (Steven ex Ledeb.) Prime Arum maculatum L. Araceae

2020 ◽  
pp. 147-152
Author(s):  
Rainer W. Bussmann ◽  
Ketevan Batsatsashvili ◽  
Zaal Kikvidze ◽  
Narel Y. Paniagua-Zambrana ◽  
Manana Khutsishvili ◽  
...  
Keyword(s):  
Arum Maculatum ◽  
Arum Italicum

Nucleotide sugars and starch synthesis in spadix of Arum maculatum and suspension cultures of Glycine max

1984 ◽  
Vol 23 (11) ◽  
pp. 2463-2468 ◽  
Author(s):  
T. ap Rees ◽  
M. Leja ◽  
F.D. Macdonald ◽  
J.H. Green
Keyword(s):  
Glycine Max ◽  
Starch Synthesis ◽  
Suspension Cultures ◽  
Nucleotide Sugars ◽  
Arum Maculatum

scholarly journals On the Development of Arum maculatum from the Seed

1898 ◽  
Vol os-12 (3) ◽  
pp. 399-414 ◽  
Author(s):  
RINA SCOTT ◽  
ETHEL SARGANT
Keyword(s):  
Arum Maculatum
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