Acid-phosphatase activity in sieve-tube members of Tilia americana

Planta ◽  
1965 ◽  
Vol 65 (2) ◽  
pp. 180-185 ◽  
Author(s):  
Hope H. Lester ◽  
Ray F. Evert
1969 ◽  
Vol 22 (4) ◽  
pp. 1051 ◽  
Author(s):  
S-Y Zee

Acid phosphatase activity has been detected within the sieve-tube members of plants by many workers using the Gomori technique and light microscopy (Lester and Evert 1965; Flinn and Smith 1967). Unfortunately the limited resolution makes it difficult to determine the specific sites of activity of the reaction product of the enzyme; recent advances in histochemical techniques for electron microscopy have made it possible to investigate more specifically the sites of localization of the acid phosphatase reaction product by using the Gomori lead nitrate technique (Goldfischer, Essner, and Novikoff 1964; Catesson and Czanenski 1967; Bowen 1968; Figier 1968; Wardrop 1968).


Author(s):  
O. T. Minick ◽  
E. Orfei ◽  
F. Volini ◽  
G. Kent

Hemolytic anemias were produced in rats by administering phenylhydrazine or anti-erythrocytic (rooster) serum, the latter having agglutinin and hemolysin titers exceeding 1:1000.Following administration of phenylhydrazine, the erythrocytes undergo oxidative damage and are removed from the circulation by the cells of the reticulo-endothelial system, predominantly by the spleen. With increasing dosage or if animals are splenectomized, the Kupffer cells become an important site of sequestration and are greatly hypertrophied. Whole red cells are the most common type engulfed; they are broken down in digestive vacuoles, as shown by the presence of acid phosphatase activity (Fig. 1). Heinz body material and membranes persist longer than native hemoglobin. With larger doses of phenylhydrazine, erythrocytes undergo intravascular fragmentation, and the particles phagocytized are now mainly red cell fragments of varying sizes (Fig. 2).


2008 ◽  
Vol 39 (6) ◽  
pp. 627-634 ◽  
Author(s):  
Tatiana Salles de Souza Malaspina ◽  
Célio Xavier dos Santos ◽  
Ana Paula Campanelli ◽  
Francisco Rafael Martins Laurindo ◽  
Mari Cleide Sogayar ◽  
...  

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