Production of pantothenic acid and thiamine by Azotobacter vinelandii in a chemically defined medium and a dialysed soil medium

1996 ◽  
Vol 22 (1-2) ◽  
pp. 131-135 ◽  
Author(s):  
Maria Victoria Martinez-Toledo ◽  
Belen Rodelas ◽  
Victoriano Salmeron ◽  
Clementina Pozo ◽  
Jesus Gonzalez-Lopez
Keyword(s):  
Azotobacter Vinelandii ◽  
Pantothenic Acid ◽  
Defined Medium ◽  
Chemically Defined Medium ◽  
Soil Medium

Production of pantothenic acid and thiamine by Azotobacter vinelandii in a chemically defined medium and a dialysed soil medium

1996 ◽  
Vol 22 (1) ◽  
pp. 131-135 ◽  
Author(s):  
Maria Victoria Martinez-Toledo ◽  
Belen Rodelas ◽  
Victoriano Salmeron ◽  
Clementina Pozo ◽  
J. Gonzalez-Lopez
Keyword(s):  
Azotobacter Vinelandii ◽  
Pantothenic Acid ◽  
Defined Medium ◽  
Chemically Defined Medium ◽  
Soil Medium

The Uptake of Vitamins by Mouse Fibroblast Cells (Strain LS) During Growth in a Chemically Defined Medium

1971 ◽  
Vol 8 (3) ◽  
pp. 701-708
Author(s):  
G. J. BLAKER ◽  
S. J. PIRT
Keyword(s):  
Folic Acid ◽  
Cell Growth ◽  
Pantothenic Acid ◽  
Defined Medium ◽  
Mouse Fibroblast ◽  
Growth Yields ◽  
Fibroblast Cells ◽  
Chemically Defined Medium ◽  
Assay Methods ◽  
Early Phases

The uptake of biotin, choline, folic acid, hypoxanthine, inositol, nicotinamide, pantothenic acid, pyridoxine, riboflavin, thiamine and vitamin B12 by mouse LS cells in suspension culture was determined by microbiological assay methods. Based on the extent of uptake during cell growth, vitamin growth yields (cells produced/unit mass of vitamin utilized) were estimated for all of the vitamins, except folic acid, thiamine and B12. The growth yields were lower during the early phases of culture. No uptakes of folic acid or B12 could be demonstrated. During the period of incubation about half of the thiamine was irretrievably lost through spontaneous decomposition.

Improved cryopreservation of domestic cat sperm in a chemically defined medium

2012 ◽  
Vol 78 (9) ◽  
pp. 2120-2128 ◽  
Author(s):  
M.M. Vick ◽  
H.L. Bateman ◽  
C.A. Lambo ◽  
W.F. Swanson
Keyword(s):  
Defined Medium ◽  
Domestic Cat ◽  
Chemically Defined Medium

scholarly journals In vitro mouse spermatogenesis with an organ culture method in chemically defined medium

PLoS ONE ◽  
2018 ◽  
Vol 13 (2) ◽  
pp. e0192884 ◽  
Author(s):  
Hiroyuki Sanjo ◽  
Mitsuru Komeya ◽  
Takuya Sato ◽  
Takeru Abe ◽  
Kumiko Katagiri ◽  
...  
Keyword(s):  
Organ Culture ◽  
Culture Method ◽  
Defined Medium ◽  
Chemically Defined Medium

Cultivation and properties of Neisseria sp. grown in chemically defined media

1972 ◽  
Vol 18 (7) ◽  
pp. 1087-1090 ◽  
Author(s):  
C. P. Kenny ◽  
B. B. Diena ◽  
R. Wallace ◽  
L. Greenberg
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Present Report ◽  
Defined Medium ◽  
Chemically Defined Medium ◽  
Chemically Defined Media ◽  
Defined Media ◽  
Specific Antisera

Neisseria Chemically Defined Medium (NCDM) has been used routinely in our laboratory for a variety of purposes. The present report describes the development of NCDM agar, wherein the NCDM base is sterilized by filtration and defined supplements and agar are added. The medium is transparent and both meningococci and gonococci grow within 72 h. When grown on NCDM agar, Types 2 and 3 gonococcal colonies tend to revert to Type 1. The serological grouping of meningococci with specific antisera is not affected by growth on this medium.Parallel investigations on the growth of these species in liquid NCDM demonstrated that the yield of Neisseria gonorrhoeae is enhanced when the medium is sterilized by filtration.

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