Localization of NaPi-1, a Na-Pi cotransporter, in rabbit kidney proximal tubules

1993 ◽  
Vol 424 (3-4) ◽  
pp. 203-209 ◽  
Author(s):  
Maria Custer ◽  
Felix Meier ◽  
Eberhard Schlatter ◽  
Rainer Greger ◽  
Arlyn Garcia-Perez ◽  
...  
Keyword(s):  
Proximal Tubules ◽  
Rabbit Kidney ◽  
Kidney Proximal Tubules

Localization of NaPi-1, a Na/Pi cotransporter, in rabbit kidney proximal tubules

1993 ◽  
Vol 424 (3-4) ◽  
pp. 210-215 ◽  
Author(s):  
J. Biber ◽  
M. Custer ◽  
A. Werner ◽  
B. Kaissling ◽  
H. Murer
Keyword(s):  
Proximal Tubules ◽  
Rabbit Kidney ◽  
Kidney Proximal Tubules

Kidney proximal tubules: Ultrastructural response to changes in calcium and adenosine triphosphate

1987 ◽  
Vol 45 ◽  
pp. 890-891
Author(s):  
B. L. Craig ◽  
A. Lefurgey ◽  
W. R. Jacobs ◽  
L. J. Mandel
Keyword(s):  
Electron Microscopy ◽  
Indirect Evidence ◽  
Proximal Tubules ◽  
Rabbit Kidney ◽  
Cellular Injury ◽  
X Ray ◽  
Transmission Electron ◽  
Direct Measurements ◽  
Mitochondrial Uncoupler ◽  
Kidney Proximal Tubules

Use of conventional transmission electron microscopy (TEM) and x-ray microanalysis (EPXMA) to identify the intracellular sites of Ca binding and release has provided valuable Information concerning the mechanism for the cellular action of Ca in a variety of cells. In the kidney much indirect evidence links Ca with cellular Injury, but direct measurements of Ca compartmentation are lacking. We report here our initial ultrastructural observations of kidney proximal tubules exposed to agents which alter the compartmentation of Ca in predictable ways. This TEM evaluation of intracellular structural compartments such as mitochondria or endoplasmic reticulum provides the basis for correlative EPXMA studies of elemental compartmentation under the same conditions.A suspension of kidney proximal tubules was isolated from the rabbit kidney as previously described and incubated at 37°C for up to 20’ with the Ca lonophores A23187 (5μM) or ionomycin (10 μM) or with the mitochondrial uncoupler FCCP (4 μM).

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