Mesodermal induction in early amphibian embryos by activin A (erythroid differentiation factor)

1990 ◽  
Vol 198 (6) ◽  
pp. 330-335 ◽  
Author(s):  
Makoto Asashima ◽  
Hiroshi Nakano ◽  
Kazunori Shimada ◽  
Kei Kinoshita ◽  
Koichi Ishii ◽  
...  
Keyword(s):  
Erythroid Differentiation ◽  
Activin A ◽  
Differentiation Factor ◽  
Amphibian Embryos ◽  
Erythroid Differentiation Factor

Inducible gene expression of activin A/erythroid differentiation factor in HL-60 cells

1990 ◽  
Vol 167 (2) ◽  
pp. 654-658 ◽  
Author(s):  
Shunji Takahashi ◽  
Takayuki Yamashita ◽  
Yuzuru Eto ◽  
Hiroshiro Shibai ◽  
Kaoru Miyamoto ◽  
...  
Keyword(s):  
Gene Expression ◽  
Erythroid Differentiation ◽  
Activin A ◽  
Inducible Gene Expression ◽  
Differentiation Factor ◽  
Erythroid Differentiation Factor ◽  
Inducible Gene

scholarly journals Expression of activin A/erythroid differentiation factor in murine bone marrow stromal cells

Blood ◽  
1992 ◽  
Vol 79 (2) ◽  
pp. 304-307 ◽  
Author(s):  
T Yamashita ◽  
S Takahashi ◽  
E Ogata
Keyword(s):  
Bone Marrow ◽  
Cell Lines ◽  
Stromal Cells ◽  
Erythroid Differentiation ◽  
Activin A ◽  
Murine Bone Marrow ◽  
Differentiation Factor ◽  
A Chain ◽  
Murine Erythroleukemia ◽  
Erythroid Differentiation Factor

Accumulating evidence suggests that activin A/erythroid differentiation factor (EDF), a homodimer of beta A chain, is a physiologic hematopoietic factor, particularly of erythroid lineage. Media conditioned by phorbol myristate acetate (PMA)-stimulated murine bone marrow stromal cell lines, MC3T3-G2/PA6 and ST2, contained activin A/EDF, assayed as the activity inducing erythroid differentiation of an activin A/EDF-responsive murine erythroleukemia cell clone F5. Follistatin, a protein specifically binding to activin A/EDF, abolished this erythroid differentiation. Northern blot analysis showed that PMA rapidly increased beta A chain messenger RNA levels in MC3T3-G2/PA6 and ST2 cells. In a search for natural stimulators, we found that tumor necrosis factor-alpha, in itself and synergistically with interleukin-1 beta, induced activin A/EDF production in both cell lines. These results indicate that stromal cells produce activin A/EDF in bone marrow.

scholarly journals Expression of activin A/erythroid differentiation factor in murine bone marrow stromal cells

Blood ◽  
1992 ◽  
Vol 79 (2) ◽  
pp. 304-307 ◽  
Author(s):  
T Yamashita ◽  
S Takahashi ◽  
E Ogata
Keyword(s):  
Bone Marrow ◽  
Cell Lines ◽  
Stromal Cells ◽  
Erythroid Differentiation ◽  
Activin A ◽  
Murine Bone Marrow ◽  
Differentiation Factor ◽  
A Chain ◽  
Murine Erythroleukemia ◽  
Erythroid Differentiation Factor

Abstract Accumulating evidence suggests that activin A/erythroid differentiation factor (EDF), a homodimer of beta A chain, is a physiologic hematopoietic factor, particularly of erythroid lineage. Media conditioned by phorbol myristate acetate (PMA)-stimulated murine bone marrow stromal cell lines, MC3T3-G2/PA6 and ST2, contained activin A/EDF, assayed as the activity inducing erythroid differentiation of an activin A/EDF-responsive murine erythroleukemia cell clone F5. Follistatin, a protein specifically binding to activin A/EDF, abolished this erythroid differentiation. Northern blot analysis showed that PMA rapidly increased beta A chain messenger RNA levels in MC3T3-G2/PA6 and ST2 cells. In a search for natural stimulators, we found that tumor necrosis factor-alpha, in itself and synergistically with interleukin-1 beta, induced activin A/EDF production in both cell lines. These results indicate that stromal cells produce activin A/EDF in bone marrow.

scholarly journals Activin A/erythroid differentiation factor is induced during human monocyte activation.

1992 ◽  
Vol 176 (5) ◽  
pp. 1449-1452 ◽  
Author(s):  
M Erämaa ◽  
M Hurme ◽  
U H Stenman ◽  
O Ritvos
Keyword(s):  
Human Peripheral Blood ◽  
Human Monocyte ◽  
Erythroid Differentiation ◽  
Activin A ◽  
Blood Monocytes ◽  
Dihydroxyvitamin D3 ◽  
Differentiation Factor ◽  
A Subunit ◽  
Activated Monocytes ◽  
Erythroid Differentiation Factor

Activin A/erythroid differentiation factor (EDF), a dimeric polypeptide hormone composed of two beta A subunits, regulates growth and erythroid differentiation of human hematopoietic progenitor and erythroleukemia cells. We have identified activated human peripheral blood monocytes as a natural source of activin A/EDF. In these cells, lipopolysaccharide (LPS) induced rapidly the expression of the beta A subunit mRNAs through protein kinase C-dependent transcriptional regulation. The beta A subunit mRNA expression was also increased by 1,25-dihydroxyvitamin D3, an inducer of macrophage maturation of monocytes. Western analysis with an anti-beta A antibody and an erythroid differentiation bioassay confirmed that the conditioned media of LPS-activated monocytes contained the activin A/EDF protein. We suggest that monocyte/macrophage-derived activin A/EDF may not only modulate hematopoiesis but may also act as a mediator molecule in the diverse physiologic and pathogenetic events in which these cells are involved.

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