Genetic analysis of the individual T-DNA genes of Agrobacterium tumefaciens; further evidence that two genes are involved in indole-3-acetic acid synthesis

1984 ◽  
Vol 194 (1-2) ◽  
pp. 265-274 ◽  
Author(s):  
D. Inzé ◽  
A. Follin ◽  
M. Van Lijsebettens ◽  
C. Simoens ◽  
C. Genetello ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Agrobacterium Tumefaciens ◽  
Genetic Analysis ◽  
Acid Synthesis ◽  
The Individual ◽  
Indole 3 Acetic Acid

scholarly journals Introduksi Gen DefH9-iaaM dan DefH9-RI-iaaM ke dalam Genom Tanaman Tomat Menggunakan Vektor Agrobacterium tumefaciens

2016 ◽  
Vol 6 (1) ◽  
pp. 18
Author(s):  
Ragapadmi S Purnamaningsih
Keyword(s):  
Acetic Acid ◽  
Agrobacterium Tumefaciens ◽  
Genetic Improvement ◽  
Plant Cells ◽  
Fruit Production ◽  
Total Efficiency ◽  
Parthenocarpic Fruit ◽  
Tomato Genome ◽  
Tomato Cultivars ◽  
Indole 3 Acetic Acid

<p>Introduction of DefH9-iaaM and DefH9-RI-iaaM Gene<br />Into Tomato Genome Using Agrobacterium tumefaciens.<br />Ragapadmi Purnamaningsih. Plant genetic improvement<br />can be conducted through genetic engineering.<br />Parthenocarpic fruit production could increase fruit<br />production and its qulities. IAA genes were introduced into<br />three tomato cultivars Ratna, Opal and LV 6117 using two<br />constract genes DefH9-iaaM and DefH9-RI-iaaM. The iaaM<br />gene is able to increase auxin biosynthesis in transgenic<br />plant cells and organs because indol-eacetamide,<br />synthesized by the product of the iaaM gene, is converted<br />either chemically or enzimatically to indole-3-acetic acid<br />(IAA), while the promotor DefH9 enable IAA gene expressed<br />specifically in the ovules. The objectives of this experiment<br />was to identify gene introduction into plant genom of three<br />tomato cultivars. The factors tested were two constract of<br />IAA genes (DefH9-iaaM or DefH9-RI-iaaM), tomato cultivars<br />(Ratna, Opal, and LV 6117) and time of explant inoculation<br />(5, 15, 30 minute). The result showed that the best time<br />inoculation was 5 minute. Otherwise three tomato cultivars<br />response better to DefH9-RI-iaaM than DefH9-iaaM. The total<br />efficiency of regeneration and total efficiency of<br />transformation of both genes were 25.38% and 20.32%. PCR<br />analysis showed that 10 plant have positive PCR, were 1<br />plant carried (Opal) DefH9-iaaM gene and 9 plant (Ratna,<br />Opal, LV 6117) carried DefH9-RI-iaaM gene.</p>

scholarly journals The Distribution of Tryptophan-Dependent Indole-3-Acetic Acid Synthesis Pathways in Bacteria Unraveled by Large-Scale Genomic Analysis

Molecules ◽  
2019 ◽  
Vol 24 (7) ◽  
pp. 1411 ◽  
Author(s):  
Pengfan Zhang ◽  
Tao Jin ◽  
Sunil Kumar Sahu ◽  
Jin Xu ◽  
Qiong Shi ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Large Scale ◽  
Growth Promotion ◽  
Plant Root ◽  
Genomic Analysis ◽  
Acid Synthesis ◽  
Metagenomic Data ◽  
Bacterial Genomes ◽  
Bacterial Phyla ◽  
Indole 3 Acetic Acid

Bacterial indole-3-acetic acid (IAA), an effector molecule in microbial physiology, plays an important role in plant growth-promotion. Here, we comprehensively analyzed about 7282 prokaryotic genomes representing diverse bacterial phyla, combined with root-associated metagenomic data to unravel the distribution of tryptophan-dependent IAA synthesis pathways and to quantify the IAA synthesis-related genes in the plant root environments. We found that 82.2% of the analyzed bacterial genomes were potentially capable of synthesizing IAA from tryptophan (Trp) or intermediates. Interestingly, several phylogenetically diverse bacteria showed a preferential tendency to utilize different pathways and tryptamine and indole-3-pyruvate pathways are most prevalent in bacteria. About 45.3% of the studied genomes displayed multiple coexisting pathways, constituting complex IAA synthesis systems. Furthermore, root-associated metagenomic analyses revealed that rhizobacteria mainly synthesize IAA via indole-3-acetamide (IAM) and tryptamine (TMP) pathways and might possess stronger IAA synthesis abilities than bacteria colonizing other environments. The obtained results refurbished our understanding of bacterial IAA synthesis pathways and provided a faster and less labor-intensive alternative to physiological screening based on genome collections. The better understanding of IAA synthesis among bacterial communities could maximize the utilization of bacterial IAA to augment the crop growth and physiological function.

The Route, Control and Compartmentation of Auxin Synthesis

1993 ◽  
Vol 20 (5) ◽  
pp. 527 ◽  
Author(s):  
HM Nonhebel ◽  
TP Cooney ◽  
R Simpson
Keyword(s):  
Amino Acids ◽  
Acetic Acid ◽  
Recent Work ◽  
Aspartate Aminotransferase ◽  
Acid Synthesis ◽  
Pea Seedlings ◽  
Auxin Synthesis ◽  
Aromatic Aminotransferase ◽  
Work Supports ◽  
Indole 3 Acetic Acid

The study of indole-3-acetic acid synthesis has undergone something of a revival recently in an attempt to understand the control of IAA levels. Results are, however, contradictory with three separate hypotheses emerging. Our own work supports older evidence for L-tryptophan as the IAA precursor and appears to simplify the metabolism of tryptophan to IAA. Work comparing incorporation of 2H from 2H2O into IAA, tryptophan, tryptamine and indole-3-pyruvate in tomato shoots showed that the indole-3-pyruvate became labelled at a rate compatible with it being the sole intermediate between tryptophan and indole-3-acetaldehyde. Results also showed that tryptamine was not involved in IAA synthesis although it was present. Indole-3-acetaldoxime was not detected in tomato shoots. An aromatic aminotransferase able to catalyse the synthesis of indole-3-pyruvate has been purified from mung beans. This enzyme was separated from aspartate aminotransferase and is fairly specific for aromatic L-amino acids. Other work, however, has implicated D-tryptophan as a more direct precursor than the L-enantiomer. A D-tryptophan aminotransferase has been isolated from dark grown pea seedlings. Finally, other recent work has indicated the existence of an alternative biosynthetic route to IAA which does not involve tryptophan. These results are reviewed in this paper and the apparent contradictions between them discussed.

Biosynthesis of indole-3-acetic acid by Agrobacterium Tumefaciens Eha-1O5, and Aspergillus Niger

2008 ◽  
Vol 136 ◽  
pp. S65-S66
Author(s):  
S. Hsbib A. Naqvi ◽  
M. Umar Dahot ◽  
Humera ◽  
Qurat-ul-Ain
Keyword(s):  
Acetic Acid ◽  
Agrobacterium Tumefaciens ◽  
Aspergillus Niger ◽  
Indole 3 Acetic Acid

The Site of Indole-3-acetic Acid Synthesis in Mesophyll Cells of Spinacia oleracea

1982 ◽  
Vol 37 (3-4) ◽  
pp. 174-178 ◽  
Author(s):  
B. Heilmann ◽  
W. Hartung ◽  
H. Gimmler
Keyword(s):  
Acetic Acid ◽  
Abscisic Acid ◽  
Acid Treatment ◽  
Spinacia Oleracea ◽  
Intracellular Localization ◽  
Acid Synthesis ◽  
Acid Biosynthesis ◽  
Mesophyll Cells ◽  
Indole 3 Acetic Acid

Abstract Using [14C]tryptophan as a precursor, the intracellular localization of indole-3-acetic acid biosynthesis in spinach mesophyll cells was investigated. Chloroplasts as well as extraplastidic compartments were able to transform tryptophan into indole-3-acetic acid.The cofactor requirement of plastidic and extraplastidic indole-3-acetic acid synthesis is shown.Light and abscisic acid treatment inhibited preparations.

scholarly journals Sulphur Analogues of Indoleacetic Acid: Synthesis and Biological Activity of some Thionaphthenacetic Acids

1957 ◽  
Vol 10 (1) ◽  
pp. 80 ◽  
Author(s):  
NP Kefford ◽  
Judith M Kelso
Keyword(s):  
Acetic Acid ◽  
Biological Activity ◽  
Plant Growth ◽  
Indoleacetic Acid ◽  
Acid Synthesis ◽  
Dichlorophenoxyacetic Acid ◽  
Protoplasmic Streaming ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Indole 3 Acetic Acid ◽  
Acetic Acids

The thionaphthen�2�, �3-, -5-, -6�, and -7-acetic acids have been synthesized from the chlorides of the corresponding carboxylic acids by the Arndt-Eistert reaction and their plant growth-regulating activities, as indicated by their effects upon protoplasmic streaming, have been compared with those of indole-3-acetic acid (IAA) and 2,4-dichlorophenoxyacetic acid (2,4�D). The thionaphthen-3- and -7- acetic acids and IAA have similar effects upon protoplasmic streaming, while the effects of the thionaphthen-2-, -5-, and �6-acetic acids differ from those of IAA and resemble in part those of 2, 4-D.

scholarly journals Indole-3-acetic Acid Synthesis in Tumorous and Nontumorous Species of Nicotiana

1978 ◽  
Vol 61 (5) ◽  
pp. 743-747 ◽  
Author(s):  
Shih-Tung Liu ◽  
Charles D. Katz ◽  
C. Arthur Knight
Keyword(s):  
Acetic Acid ◽  
Acid Synthesis ◽  
Indole 3 Acetic Acid
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