The cytochemical localization of lysosomal hydrolases in the digestive cells of littorinids and changes induced by larval trematode infection

1977 ◽  
Vol 53 (1) ◽  
pp. 115-122 ◽  
Author(s):  
M. N. Moore ◽  
D. W. Halton
Author(s):  
W. Allen Shannon ◽  
Yoshinobu Hoshino ◽  
Arnold M. Seligman

The metal salt capture methods for acid phosphatase and alkaline phosphatase with numerous variations based principally on the Gomori method have been useful methods for direct cytochemical visualization of the enzymes. However, the possibilities of nonenzymatic deposition of reaction product, possible inhibition of enzymatic activities, and other suggested weaknesses of the methods which are found in the literature give reason to develop a new approach to the cytochemical localization of these enzymes. Other attempts along these lines have thus far been more or less partially successful. More recently Hanker et al. have demonstrated lysosomal hydrolases by the formation of osmium blacks based on synthetic substrates which initially deposit Hatchett's brown (cupric ferrocyanide) at the reactive sites. The synthetic substrate', 2-naph-thylthiolphosphate (NTP), developed by Seligman et al. to use in the demonstration of acid and alkaline phosphatase and subsequently used by Hanker et al.


1961 ◽  
Vol 47 (3) ◽  
pp. 445
Author(s):  
Kathleen L. Hussey ◽  
Walter B. Stahl

1966 ◽  
Vol 14 (1) ◽  
pp. 49 ◽  
Author(s):  
WH Ewers ◽  
CR Rose

Velacumantus australis is a common gastropod along the Australian coast. Samples of four populations were examined and the frequency of two banded forms was recorded. All brown-banded snails were small juveniles and these apparently die before reaching adolescence. White-banded snails occurred in all age groups and their frequency tends to be highest in juveniles and lowest in the oldest adults. White-banded snails have a much lower incidence of larval trematode infection than unbanded snails and also differ slightly in weight and in the onset of sexual maturity. It is suggested that these forms are maintained as a selectively balanced polymorphism.


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