Salivary cortisol for monitoring circadian rhythm variation in adrenal activity during shiftwork

1993 ◽  
Vol 64 (7) ◽  
pp. 499-502 ◽  
Author(s):  
S. Shinkai ◽  
S. Watanabe ◽  
Y. Kurokawa ◽  
J. Torii
Keyword(s):  
Circadian Rhythm ◽  
Salivary Cortisol

Lack of Adult-Type Salivary Cortisol Circadian Rhythm in Hospitalized Preterm Infants

2005 ◽  
Vol 64 (1) ◽  
pp. 20-27 ◽  
Author(s):  
Susan Kidd ◽  
Paula Midgley ◽  
Mary Nicol ◽  
John Smith ◽  
Neil McIntosh
Keyword(s):  
Circadian Rhythm ◽  
Preterm Infants ◽  
Salivary Cortisol ◽  
Adult Type

Abstract P160: Non-invasive Method to Assess Human Circadian Rhythms

Hypertension ◽  
2016 ◽  
Vol 68 (suppl_1) ◽  
Author(s):  
Daian Chen ◽  
S Justin Thomas ◽  
David A Calhoun ◽  
David M Pollock ◽  
Jennifer S Pollock
Keyword(s):  
Circadian Rhythm ◽  
Circadian Rhythms ◽  
Salivary Cortisol ◽  
Metabolic Diseases ◽  
Clock Genes ◽  
Light Exposure ◽  
Rna Isolation ◽  
Buccal Cells ◽  
Non Invasive ◽  
Salivary Melatonin

Circadian rhythms are controlled by an endogenous time-keeping system oscillating approximately on a 24-h cycle under constant conditions. These rhythms depend on a network of interacting genes and proteins, including transcriptional activators such as CLOCK, NPAS2, and ARNTL (BMAL1), which induce transcription of the clock genes Period ( Per1 , Per2 , and Per3 ) and Cryptochrome ( Cry1 and Cry2 ). Human salivary cortisol and melatonin follow a clear circadian rhythm as well. Disruption of the circadian rhythm and sleep-wake cycles are considered risk factors for a variety of health problems, especially hypertension and other cardiovascular and metabolic diseases. Here we put together practical methods for assessing circadian rhythms in adult subjects conducted by each individual. This method is non-invasive, inexpensive and provides a predictive profile of an individual’s circadian rhythm related to clock-controlled gene expression in buccal cells, salivary cortisol, salivary melatonin, and subject’s activity or sleep. Subjects are instructed on how to obtain buccal cells using swabs (Whatman OmniSwab) from the inside of their cheeks and collect saliva using salivettes (Sarstedt) every 4 hours starting at 6am, for 2 consecutive days. Subjects also wear actigraphy watches (Phillips Respironics) during the 2 days, to record their activity, light exposure and estimates of sleep times. To monitor adherence to correct time point collections, each subject is given an electronic vial called eCAP (Information Mediary Corp) that records the exact time the container is opened to place samples once collected. We demonstrate feasibility to extract up to 150ng/μl of RNA (Ambion RNAqueous-Micro Total RNA Isolation Kit) from buccal cells swabs. Salivary melatonin and cortisol are measured by radioimmunoassay (Buhlmann Lab) with melatonin peak levels ranging from 14 to 23 pg/ml and cortisol peak levels ranging from 10 to 24 ng/ml. We suggest that buccal cell expression of clock-controlled genes, salivary melatonin, salivary cortisol, and actigraphy data are valuable in providing reliable assessment of human circadian rhythm profiles under a variety of conditions.

scholarly journals SALIVARY CORTISOL AND METABOLIC SYNDROME COMPONENT’S ASSOCIATION

2018 ◽  
Vol 31 (1) ◽  
Author(s):  
Grasiane Izidorio GARBELLOTTO ◽  
Fernanda Jardim REIS ◽  
Ana Maria Pandolfo FEOLI ◽  
Carla Haas PIOVESAN ◽  
Andréia da Silva GUSTAVO ◽  
...  
Keyword(s):  
Physical Activity ◽  
Risk Factors ◽  
Metabolic Syndrome ◽  
Circadian Rhythm ◽  
Salivary Cortisol ◽  
Cross Sectional Study ◽  
Elevated Blood ◽  
Sectional Study ◽  
Cross Sectional ◽  
Pituitary Adrenal Axis

ABSTRACT Background: Actually the lifestyle exposes the population to several risk factors related to alimentary habits and less physical activity that contributes to chronic diseases appearance worldwide. Aim: To analyze the association between salivary cortisol and the components of metabolic syndrome. Methods: This is a cross-sectional study. As part of it, 28 individuals aged 30-59 years presenting three or more of the following findings: CA: ≥88 cm for women and ≥102 cm for men; SBP>130 mmHg and DBP>85 mmHg; GL>100 mg/dl; TG>150 mg/dl; HDL<40 mg/dl for men and <50 mg/dl for women. Was performed analysis of salivary cortisol (by radioimmunoassay) from 25 salivary samples collected throughout the day, for evaluating changes in the circadian rhythm of this hormone (8AM, noon and 8PM). Results: 28 evaluated individuals had a mean age of 51.9±7.5 years, mostly women (64.3%) and a mean of BMI 33.6±3.2 kg/m². The cortisol level from the 8AM averaged 18.7±4.8 ng/dlL. Individuals with FPG>110mg/dl, have significantly lower average levels of cortisol than ones with FPG <110 (12.8±5,2 vs. 17.3±4.2). Significant correlations were HOMA vs. WC (r=0,465; p˂0,005) and TG (r=0,473; p˂0,005), WC vs. FG (r=0,446; p˂0,005) and BMI (r=0,730; p˂0.0001); TG vs. HDL (r=0,441 p˂0,005) and FG (r=0,440; p˂0,005). Conclusion: Morning salivary cortisol in subjects with chronically elevated blood glucose can represent a downregulation of the hypothalamic-pituitary adrenal axis. This is an important finding not yet well investigated.

scholarly journals Age of appearance of circadian rhythm in salivary cortisol values in infancy.

1983 ◽  
Vol 58 (6) ◽  
pp. 454-456 ◽  
Author(s):  
D A Price ◽  
G C Close ◽  
B A Fielding
Keyword(s):  
Circadian Rhythm ◽  
Salivary Cortisol

scholarly journals A Study on Comparison of Salivary Cortisol Circadian Rhythm in Periodontal Diseases with External Stressors and Clinical Parameters

2016 ◽  
Vol 9 (2) ◽  
pp. 679-688
Author(s):  
Mathew Asok ◽  
Prabhu Natarajan ◽  
Sheikha Saeed ◽  
Majd Khalid ◽  
Nisha Varughese ◽  
...  
Keyword(s):  
Circadian Rhythm ◽  
Salivary Cortisol ◽  
Periodontal Diseases ◽  
Clinical Parameters

scholarly journals Chronobiological Factors Related to Sleep and Neuropsychiatric Symptoms in Persons Living With Dementia

2021 ◽  
Vol 5 (Supplement_1) ◽  
pp. 436-437
Author(s):  
Nancy Hodgson ◽  
Fanghong Dong
Keyword(s):  
Circadian Rhythm ◽  
White Light ◽  
Salivary Cortisol ◽  
Light Exposure ◽  
Neuropsychiatric Symptoms ◽  
Clear Understanding ◽  
Diurnal Cortisol ◽  
Endogenous Factors ◽  
Subjective Sleep ◽  
Rhythm Pattern

Abstract Circadian rhythm disturbances (CRD) are commonly seen in people living with dementia. A clear understanding of the role of CRD in dementia etiology will be beneficial by exploring the exogenous factors (externally influence the duration of sleep hours, such as light/dark cycles) and endogenous factors (internal biological rhythm, such as diurnal cortisol pattern). This symposium will apply a chronobiological approach to study exogenous and endogenous factors that influence circadian rhythm and their effects on sleep and neuropsychiatric symptoms in persons living with dementia (PLWD). Four paper presentations will use secondary data analysis of data from the Healthy Patterns Clinical Trial (NCT03682185), a randomized controlled trial of a home-based activity intervention designed to improve circadian rhythm disorders in PLWD. We will first describe the circadian rhythm pattern reflected by endogenous factors (salivary cortisol), then examine salivary cortisol (endogenous) and white light intensity (exogenous) and on subjective sleep and neuropsychiatric symptoms (including depression) in PLWD, respectively. In session 1, we will present cortisol diurnal rhythm pattern in PLWD using a cross-sectional design. In session 2, we will discuss the relationship between salivary cortisol indicators and depressive symptoms. In session 3, we focus on the association between diurnal cortisol slope and neuropsychiatric symptoms using the baseline data. In session 4, we describe the association between evening white light exposure and subjective sleep. The discussant will describe how these findings build on our understanding the nature of circadian rhythm disturbance in dementia and inform future research and treatment approaches.

scholarly journals Assessment of exercise-induced stress by automated measurement of salivary cortisol concentrations within the circadian rhythm in Japanese female long-distance runners

2020 ◽  
Author(s):  
Kazumi Ushiki ◽  
Katsuhiko Tsunekawa ◽  
Yoshifumi Shoho ◽  
Larasati Martha ◽  
Hirotaka Ishigaki ◽  
...  
Keyword(s):  
Circadian Rhythm ◽  
Salivary Cortisol ◽  
Serum Cortisol ◽  
Early Morning ◽  
Rate Of Change ◽  
Exercise Stress ◽  
Enzyme Linked Immunosorbent Assay ◽  
Distance Runners ◽  
Long Distance ◽  
Overtraining Syndrome

Abstract Background Overtraining syndrome, caused by prolonged excessive stress, results in reduced performance and cortisol responsiveness in athletes. It is necessary to collect saliva samples sequentially within circadian rhythm for assessing exercise stress by measuring cortisol concentrations, and automated cortisol measurements using electrochemiluminescence immunoassay (ECLIA) may be useful for measuring a large number of saliva samples. In this study, we evaluated the appropriate use of cortisol-based exercise stress assessment within the circadian rhythm, which may diagnose and prevent overtraining syndrome in athletes. Methods We collected saliva and sera from 54 healthy participants and analyzed the correlation between salivary cortisol concentrations measured by ECLIA and enzyme-linked immunosorbent assay (ELISA) or serum cortisol analysis. We also collected saliva continuously from 12 female long-distance runners on 2 consecutive days involving different intensities and types of exercise early in the morning and in the afternoon and measured salivary cortisol concentrations using ECLIA. Each exercise intensity of runners was measured by running velocities, Borg Scale score, and rate of change in the pulse rate by exercise. Results ECLIA-based salivary cortisol concentrations correlated positively with those detected by ELISA (ρ = 0.924, p < 0.001) and serum cortisol (ρ = 0.591, p = 0.001). In long-distance runners, circadian rhythm of salivary cortisol including peak after waking and decrease promptly thereafter were detected on both days by continuous saliva sampling. The rates of change in salivary cortisol concentrations were significantly lower after an early morning exercise than after an afternoon exercise on both days (day 1, p = 0.002 and day 2, p = 0.003). In the early morning exercise, the rate of change in salivary cortisol concentration was significantly higher on day 1 than on day 2 ( p = 0.034), similar to significant difference in running velocities ( p = 0.001). Conclusions Our results suggest that automated ECLIA-based salivary cortisol measurements are able to detect the athletes' circadian rhythm and compare the exercise stress intensities at the same times on different days, even in the early morning, possibly leading to prevention of overtraining syndrome.

Determination of Salivary Cortisol by ELISA and Its Application to the Assessment of the Circadian Rhythm in Children

1995 ◽  
Vol 44 (5) ◽  
pp. 213-217 ◽  
Author(s):  
Mieko Shimada ◽  
Kiyohisa Takahashi ◽  
Tomi Ohkawa ◽  
Masaya Segawa ◽  
Makoto Higurashi
Keyword(s):  
Circadian Rhythm ◽  
Salivary Cortisol
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