Isolation and characterization of dominant, pleiotropic drug-resistance mutants in Chlamydomonas reinhardtii

1989 ◽  
Vol 15 (6) ◽  
pp. 443-452 ◽  
Author(s):  
Steven W. James ◽  
Paul A. Lefebvre
Keyword(s):  
Drug Resistance ◽  
Chlamydomonas Reinhardtii ◽  
Pleiotropic Drug Resistance ◽  
Isolation And Characterization ◽  
Resistance Mutants

scholarly journals New Insights into the Pleiotropic Drug Resistance Network from Genome-Wide Characterization of the YRR1 Transcription Factor Regulation System

2002 ◽  
Vol 22 (8) ◽  
pp. 2642-2649 ◽  
Author(s):  
Stéphane Le Crom ◽  
Frédéric Devaux ◽  
Philippe Marc ◽  
Xiaoting Zhang ◽  
W. Scott Moye-Rowley ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Transcription Factor ◽  
Binding Site ◽  
Time Course ◽  
Target Genes ◽  
Regulation System ◽  
Dependent Manner ◽  
Pleiotropic Drug Resistance ◽  
Genome Wide

ABSTRACT Yrr1p is a recently described Zn2Cys6 transcription factor involved in the pleiotropic drug resistance (PDR) phenomenon. It is controlled in a Pdr1p-dependent manner and is autoregulated. We describe here a new genome-wide approach to characterization of the set of genes directly regulated by Yrr1p. We found that the time-course production of an artificial chimera protein containing the DNA-binding domain of Yrr1p activated the 15 genes that are also up-regulated by a gain-of-function mutant of Yrr1p. Gel mobility shift assays showed that the promoters of the genes AZR1, FLR1, SNG1, YLL056C, YLR346C, and YPL088W interacted with Yrr1p. The putative consensus Yrr1p binding site deduced from these experiments, (T/A)CCG(C/T)(G/T)(G/T)(A/T)(A/T), is strikingly similar to the PDR element binding site sequence recognized by Pdr1p and Pdr3p. The minor differences between these sequences are consistent with Yrr1p and Pdr1p and Pdr3p having different sets of target genes. According to these data, some target genes are directly regulated by Pdr1p and Pdr3p or by Yrr1p, whereas some genes are indirectly regulated by the activation of Yrr1p. Some genes, such as YOR1, SNQ2, and FLR1, are clearly directly controlled by both classes of transcription factor, suggesting an important role for the corresponding membrane proteins.

scholarly journals Complete Genome Sequence of Klebsiella pneumoniae Myophage Mineola

2019 ◽  
Vol 8 (17) ◽  
Author(s):  
Justin X. Boeckman ◽  
Lauren Lessor ◽  
Jason J. Gill ◽  
Mei Liu
Keyword(s):  
Drug Resistance ◽  
Klebsiella Pneumoniae ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Human Pathogen ◽  
Content Type ◽  
Isolation And Characterization ◽  
Wide Range

Klebsiella pneumoniae is an important human pathogen due to the wide range of infections it can cause and its emerging drug resistance. Isolation and characterization of phage infecting K. pneumoniae could be important for future therapeutic applications.

Isolation and Characterization of Chemotaxis Mutants of Chlamydomonas reinhardtii

1996 ◽  
Vol 32 (6) ◽  
pp. 357-359 ◽  
Author(s):  
Elena V. Ermilova ◽  
Elena M. Chekunova ◽  
Zhanna M. Zalutskaya ◽  
Konstantin R. Krupnov ◽  
Boris V. Gromov
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Isolation And Characterization

Isolation and characterization of cDNA sequences controlled by inorganic phosphate in Chlamydomonas reinhardtii

Plant Science ◽  
1993 ◽  
Vol 89 (1) ◽  
pp. 55-67 ◽  
Author(s):  
Françoise Dumont ◽  
Bernard Joris ◽  
Aysel Gumusboga ◽  
Marc Bruyninx ◽  
Roland Loppes
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Inorganic Phosphate ◽  
Cdna Sequences ◽  
Isolation And Characterization
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