Microelectrode determination of oxyntic cell pH in intact frog gastric mucosa. Effect of histamine

1992 ◽  
Vol 422 (3) ◽  
pp. 253-259 ◽  
Author(s):  
Lucantonio Debellis ◽  
Silvana Curci ◽  
Eberhard Fr�mter
Keyword(s):  
Cell Ph ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 42-47
Author(s):  
Tien Nguyen Huu ◽  
Tram Le Thi Bao ◽  
Ngoc Nguyen Thi Nhu ◽  
Thang Phan Phuoc ◽  
Khan Nguyen Viet

Background: Curcumin is a major ingredient in turmeric (Curcuma longa L., Zingiberaceae), which has important activities such as anti-tumor, anti-inflammatory, antioxidant, anti-ischemia, protection of gastric mucosa etc,. Curcumin can be considered as a biological marker of turmeric and turmeric products. Objectives: Developing an HPLC method for quantification of curcumin in turmeric powder and turmeric - honey ball pills; applying this method for products on the market. Materials and methods: turmeric powder and turmeric - honey ball pills collected in Thua Thien Hue province. After optimization process, the method was validated and applied to evaluate the content of curcumin. Results: The chromatography analysis was performed with: Zorbaz Eclipse XDB-C18 (150 × 4.6 nm; 5 µm); Mobile phase: acetonitril: 2% acetic acid (45:55), Flow rate was kept constant at 1.0 ml/min; Detector PDA (420 nm). The method was validated for the HPLC system compatibility, specificity, linearity range, precision and accuracy; the recovery greater than 98%. Conclusion: The developed HPLC method can determine curcumin in turmeric powder and turmeric - honey ball pills. Key words: Curcumin, turmeric powder, turmeric-honey ball pills, quantitative determination, HPLC


1957 ◽  
Vol 35 (1) ◽  
pp. 729-732
Author(s):  
K. Kowalewski

The endocrine and exocrine activity of guinea pig stomach was measured by the determination of pepsinogen in gastric tissue and in plasma. Gastric juice pepsin was also studied.A significant increase of both pepsinogen and pepsin was found in animals treated with a dose of histamine (75 mg. per kg. of body weight). These results give further evidence that the zymogenic cells of gastric mucosa may be stimulated by histamine. The determination of pepsinogen in gastric tissue seems to permit a direct approach to the enzymatic function of zymogenic cells.


1981 ◽  
Vol 135 (4) ◽  
pp. 371-378 ◽  
Author(s):  
KENZO KOBAYASHI ◽  
TETSUO ARAKAWA ◽  
HAJIME NAKAMURA ◽  
SHINJI CHONO ◽  
HIROAKI YAMADA ◽  
...  

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