Effect of a ?-adrenergic agonist on glucose transport and insulin-responsive glucose transporters (GLUT4) in brown adipose tissue of control and obese fa/fa rats

1992 ◽  
Vol 421 (1) ◽  
pp. 52-58 ◽  
Author(s):  
F. Assimacopoulos-Jeannet ◽  
R. Greco-Perotto ◽  
J. Terrettaz ◽  
M. K. Meier ◽  
B. Jeanrenaud
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Glucose Transport ◽  
Glucose Transporters ◽  
Adrenergic Agonist ◽  
Brown Adipose

scholarly journals Activities of enzymes of glycerolipid synthesis in brown adipose tissue after treatment of rats with the adrenergic agonists BRL 26830A and phenylephrine, after exposure to cold and in streptozotocin-diabetes

1991 ◽  
Vol 277 (3) ◽  
pp. 665-669 ◽  
Author(s):  
J R D Mitchell ◽  
E D Saggerson
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Streptozotocin Diabetes ◽  
Fatty Acyl ◽  
Adrenergic Agonist ◽  
Interscapular Brown Adipose Tissue ◽  
Exposure To Cold ◽  
Brown Adipose ◽  
Glycerolipid Synthesis ◽  
Beta Adrenergic Agonist

1. Measurements were made, relative to tissue DNA, of the activities of enzymes of glycerolipid synthesis in homogenates of interscapular brown adipose tissue. These were: mitochondrial and microsomal forms of glycerolphosphate acyltransferase (GPAT), Mg(2+)-dependent phosphatidate phosphohydrolase (PPH) and fatty acyl-CoA synthetase (FAS). 2. In normal animals, 3 days of cold-exposure (4 degrees C) increased all activities. The increase in mitochondrial GPAT activity was particularly pronounced (5-fold). Administration of the beta-adrenergic agonist BRL 26830A mimicked the effect of cold on microsomal GPAT activity. Mitochondrial GPAT, PPH and FAS activities were unresponsive to BRL 26830A. The alpha-adrenergic agonist phenylephrine significantly decreased activities of GPAT and PPH. 3. Streptozotocin-diabetes decreased mitochondrial GPAT activity, but did not abolish the effect of cold to increase this activity or the activity of microsomal GPAT. Diabetes abolished the effect of cold on PPH and FAS activities. 4. The findings are relevant to signals that drive early events in mitochondriogenesis and cell proliferation in brown adipose tissue on exposure to cold.

scholarly journals Effect of suckling and adrenergic stimulation on peripheral deiodination in lactating rats: differential expression of type 1 deiodinase mRNA forms

2001 ◽  
Vol 171 (3) ◽  
pp. 533-540 ◽  
Author(s):  
C Aceves ◽  
R Rojas-Huidobro
Keyword(s):  
Adipose Tissue ◽  
Mammary Gland ◽  
Brown Adipose Tissue ◽  
Adrenergic Stimulation ◽  
Adrenergic Agonist ◽  
Large Form ◽  
Brown Adipose ◽  
Sympathetic Nervous ◽  
Beta Adrenergic Agonist

Previous works led us to propose that peripheral iodothyronine deiodination is mainly regulated by the reciprocal interaction between the thyroid and the sympathetic nervous system (SNS). In this study, we analyzed the role suckling exerts, through SNS activation, upon deiodination of thyronines in liver, heart, brown adipose tissue and mammary gland during lactation. Our results showed that resuckling causes a concurrent stimulatory response on deiodinase type 1 (D1) in heart and mammary gland, but not in liver and brown adipose tissue. The stimulatory response was mimicked by norepinephrine and by the beta-adrenergic agonist isoproterenol, through the overexpression of the large form of D1 mRNA. These results suggested that, during lactation, peripheral thyronine deiodination is co-ordinated by the SNS, and suckling is a major modulatory influence.

Discrepancies between the affinities of binding and action of the novel β-adrenergic agonist BRL 37344 in rat brown adipose tissue

1988 ◽  
Vol 156 (1) ◽  
pp. 375-382 ◽  
Author(s):  
Patrick Muzzin ◽  
Josiane Seydoux ◽  
Jean-Paul Giacobino ◽  
John-Craig Venter ◽  
Claire Fraser
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
The Novel ◽  
Adrenergic Agonist ◽  
Brown Adipose ◽  
Brl 37344

Defective metabolic effects of norepinephrine and insulin in obese Zucker rat brown adipose tissue

1990 ◽  
Vol 258 (2) ◽  
pp. E320-E328
Author(s):  
A. Marette ◽  
A. Geloen ◽  
A. Collet ◽  
L. J. Bukowiecki
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Glucose Transport ◽  
Metabolic Effects ◽  
Brown Adipocyte ◽  
Zucker Rats ◽  
Maximal Velocity ◽  
Brown Adipocytes ◽  
Brown Adipose ◽  
Obese Rats

The effects of insulin and norepinephrine on oxygen consumption, lipolysis, and glucose transport were investigated in adipocytes isolated from brown adipose tissue (BAT) of adult (4-5 mo) lean (Fa/?) and obese (fa/fa) Zucker rats. Total BAT protein content and cytochrome oxidase activity were similar in both phenotypes, suggesting that obese rats have a normal mitochondrial content. Light and electron micrographs revealed that brown adipocytes from obese rats contained very large multilocular triglyceride droplets, but their mitochondrial ultrastructure was normal. Norepinephrine, when added in excess (1 microM), stimulated brown adipocyte respiration 8-10 times above basal levels both in lean and obese animals. However, dose-response experiments disclosed that the 50% effective concentration (EC50) was significantly higher in cells isolated from obese rats compared with lean ones (EC50 115 vs. 43 nM, P less than 0.05). The lipolytic sensitivity to norepinephrine was also reduced in adipocytes isolated from obese animals (EC50 83 vs. 12 nM, P less than 0.05). Addition of dibutyryl adenosine 3',5'-cyclic monophosphate to respiring obese rat brown adipocytes restored to normal the defective response to norepinephrine, suggesting that the reduction in catecholamine sensitivity resulted from a deactivation of the receptor-adenylate cyclase complex. On the other hand, the antilipolytic and antithermogenic actions of physiological concentrations of insulin were significantly reduced in obese BAT cells. The sensitivity and responsiveness of obese rat brown adipocytes for insulin-stimulated glucose transport were also markedly decreased (EC50 1 vs. 0.3 nM, P less than 0.05; maximal velocity 3-fold vs. 7-fold).(ABSTRACT TRUNCATED AT 250 WORDS)

Apparent lack of beta 3-adrenoceptors and of insulin regulation of glucose transport in brown adipose tissue of guinea pigs

1995 ◽  
Vol 268 (1) ◽  
pp. R98-R104 ◽  
Author(s):  
J. Himms-Hagen ◽  
J. Triandafillou ◽  
N. Begin-Heick ◽  
M. Ghorbani ◽  
A. L. Kates
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Cold Acclimation ◽  
Glucose Transport ◽  
Guinea Pigs ◽  
Adrenergic Agonists ◽  
Specific Expression ◽  
Brown Adipocytes ◽  
Apparent Lack ◽  
Brown Adipose

Norepinephrine-induced thermogenesis was substantial in adipocytes from brown adipose tissue (BAT) of cold-acclimated guinea pigs but absent in adipocytes from BAT of warm-acclimated guinea pigs. There was no thermogenic response to any beta 3-adrenergic agonist (CL-316,243, ZD-7114, BRL-28410, CGP-12177). The receptor was characterized as a beta 1-adrenoceptor. Adrenergic agonists stimulated adenylate cyclase in membranes from BAT of both warm- and cold-acclimated guinea pigs also via a beta 1-adrenoceptor; beta 3-adrenergic agonists had no effect. Glucose transport by brown adipocytes from warm-acclimated guinea pigs was not stimulated by either norepinephrine or insulin. Cold acclimation induced the appearance of stimulation of glucose transport by norepinephrine in association with the appearance of a large capacity for thermogenesis, but there was little improvement in response to insulin. GLUT4 was present in membranes from BAT of both warm- and cold-acclimated guinea pigs. Insulin is known to have an antilipolytic effect on both BAT and white adipose tissue of guinea pigs. Thus there is a selective lack of insulin-regulated glucose transport that is not improved by cold acclimation. Guinea pigs may have a mutated component of the translocation mechanism for GLUT4. beta 3-Adrenoceptors appear to be absent in brown adipocytes of adult guinea pigs, as in white adipocytes of guinea pigs, yet are known to be present in the gut. Tissue-specific expression of beta 3-adrenergic receptors in guinea pigs may differ from that in rats, in which receptors are expressed in the adipose tissues and gut.

scholarly journals Beta-3 adrenergic agonist, BRL-26830A, and alpha/beta blocker, arotinolol, markedly increase regional blood flow in the brown adipose tissue in anesthetized rats.

1992 ◽  
Vol 56 (9) ◽  
pp. 936-942 ◽  
Author(s):  
HAKUO TAKAHASHI ◽  
TOSHIHIDE YOSHIDA ◽  
MASATO NISHIMURA ◽  
TADASHI NAKANISHI ◽  
MOTOHARU KONDO ◽  
...  
Keyword(s):  
Blood Flow ◽  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Beta Blocker ◽  
Regional Blood Flow ◽  
Adrenergic Agonist ◽  
Anesthetized Rats ◽  
Brown Adipose ◽  
Alpha Beta
Sign in / Sign up

Export Citation Format

Share Document