Ca2+ sensitizing effects of EMD 53998 after troponin replacement in skinned fibres from porcine atria and ventricles

1995 ◽  
Vol 430 (2) ◽  
pp. 220-229 ◽  
Author(s):  
Zacharias Barth ◽  
John D. Strauss ◽  
Susanne Heyder ◽  
Jennifer Van Eyk ◽  
Rudolf J. Wiesner ◽  
...  
Keyword(s):  
Skinned Fibres ◽  
Emd 53998

Studies on Water in Barnacle Muscle Fibres II. Role of ions and Organic Solutes in Swelling of Chemically-skinned Fibres

1981 ◽  
Vol 90 (1) ◽  
pp. 43-63
Author(s):  
M. E. CLARK ◽  
J. A. M. HINKE ◽  
M. E. TODD
Keyword(s):  
Water Content ◽  
Fibre Volume ◽  
Tween 20 ◽  
Muscle Fibres ◽  
Skinned Fibres ◽  
Organic Solutes ◽  
Net Charge ◽  
Trimethylamine Oxide ◽  
Organic Solute

Single muscle fibres from the giant barnacle, Balanus nubilis, were chemically skinned (2% Tween 20), then equilibrated for 40 h in salt solutions ranging in ionic strength from 0·025 to 0·6M at pH 7·0. The water content of the fibres and the net charge on the myofilaments increased with increasing salt concentration. Cation accumulation in the fibres was about equal to anion exclusion at all salt concentrations. When an organic solute (trimethylamine oxide, glycine, alanine, serine, proline, or glycerol) in the concentration range from 0·1 to 0·6 M was added to the salt solution, cation accumulation increased and water content decreased. Myofilament architecture was disrupted when the fibres were equilibrated in high salt (> 0·4 M) solutions and preserved when 0·5 M-triethylamine oxide was also added. The results are consistent with the view that organic solutes enhance the association between the fixed charge sites and their counterions. This hypothesis is examined quantitatively using the Oosawa relationship between the volume and the counterion association for cylindrical polyelectrolytes. The results illustrate that organic solutes can influence fibre volume in a way other than through osmo-regulation.

Some mechanical properties of skinned fibres of pregnant human myometrium

1994 ◽  
Vol 56 (1) ◽  
pp. 55-62 ◽  
Author(s):  
Hidetaka Izumi ◽  
R.E. Garfield ◽  
Fujio Morishita ◽  
Koichi Shirakawa
Keyword(s):  
Mechanical Properties ◽  
Human Myometrium ◽  
Skinned Fibres
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