Model of bicarbonate secretion by resting frog stomach fundus mucosa. II. Role of the oxyntopeptic cells

1994 ◽  
Vol 428 (5-6) ◽  
pp. 655-663 ◽  
Author(s):  
Lucantonio Debellis ◽  
Claudia Iacovelli ◽  
Eberhard Fr�mter ◽  
Silvana Curci
Keyword(s):  
Bicarbonate Secretion ◽  
Stomach Fundus ◽  
Frog Stomach

Model of bicarbonate secretion by resting frog stomach fundus mucosa I. Transepithelial measurements

1994 ◽  
Vol 428 (5-6) ◽  
pp. 648-654 ◽  
Author(s):  
Silvana Curci ◽  
Lucantonio Debellis ◽  
Rossella Caroppo ◽  
Eberhard Fr�mter
Keyword(s):  
Bicarbonate Secretion ◽  
Stomach Fundus ◽  
Frog Stomach

scholarly journals The role of cGMP-cGKI-signaling for duodenal bicarbonate secretion

2009 ◽  
Vol 9 (S1) ◽  
Author(s):  
Beate Spiessberger ◽  
Pascal Weinmeister ◽  
Franz Hofmann ◽  
Claudia Werner ◽  
Dieter Saur ◽  
...  
Keyword(s):  
Bicarbonate Secretion ◽  
Duodenal Bicarbonate Secretion

Protein kinase C potentiates cAMP-stimulated mouse duodenal mucosal bicarbonate secretion in vitro

2004 ◽  
Vol 286 (5) ◽  
pp. G814-G821 ◽  
Author(s):  
Bi-Guang Tuo ◽  
Jimmy Y. C. Chow ◽  
Kim E. Barrett ◽  
Jon I. Isenberg
Keyword(s):  
Short Circuit ◽  
Short Circuit Current ◽  
Duodenal Mucosa ◽  
Bicarbonate Secretion ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Ussing Chambers ◽  
Duodenal Bicarbonate Secretion

PKC has been shown to regulate epithelial Cl- secretion in a variety of models. However, the role of PKC in duodenal mucosal bicarbonate secretion is less clear. We aimed to investigate the role of PKC in regulation of duodenal mucosal bicarbonate secretion. Bicarbonate secretion by murine duodenal mucosa was examined in vitro in Ussing chambers using a pH-stat technique. PKC isoform expression and activity were assessed by Western blotting and in vitro kinase assays, respectively. PMA (an activator of PKC) alone had no effect on duodenal bicarbonate secretion or short-circuit current ( Isc). When PMA and dibutyryl-cAMP (db-cAMP) were added simultaneously, PMA failed to alter db-cAMP-stimulated duodenal bicarbonate secretion or Isc ( P > 0.05). However, a 1-h preincubation with PMA potentiated db-cAMP-stimulated duodenal bicarbonate secretion and Isc in a concentration-dependent manner (from 10-8 to 10-5M) ( P < 0.05). PMA preincubation had no effects on carbachol- or heat-stable toxin-stimulated bicarbonate secretion. Western blot analysis revealed that PKCα, -γ, -ϵ, -θ, -μ, and -ι/λ were expressed in murine duodenal mucosa. Ro 31–8220 (an inhibitor active against PKCϵ, -α, -β, and -γ), but not Gö 6983 (an inhibitor active against PKCα, -γ, -β, and -δ), reversed the potentiating effect of PMA on db-cAMP-stimulated bicarbonate secretion. PMA also time- and concentration-dependently increased the activity of PKCϵ, an effect that was prevented by Ro 31–8220 but not Gö 6983. These results demonstrate that activation of PKC potentiates cAMP-stimulated duodenal bicarbonate secretion, whereas it does not modify basal secretion. The effect of PKC on cAMP-stimulated bicarbonate secretion is mediated by the PKCϵ isoform.

Role of Protein Kinases on Acid-Induced Duodenal Bicarbonate Secretion in Rats

2002 ◽  
pp. 139-142
Author(s):  
Osamu Furukawa ◽  
Masahiko Hirokawa ◽  
Paul H. Guth ◽  
Eli Engel ◽  
Jonathan D. Kaunitz
Keyword(s):  
Protein Kinases ◽  
Bicarbonate Secretion ◽  
Duodenal Bicarbonate Secretion

Role of Protein Kinase C in Duodenal Mucosal Bicarbonate Secretion in the Guinea Pig

Pharmacology ◽  
1996 ◽  
Vol 53 (1) ◽  
pp. 60-65 ◽  
Author(s):  
H.S. Odes ◽  
R. Reimer ◽  
R. Muallem ◽  
M. Schwenk ◽  
W. Beil ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Guinea Pig ◽  
Bicarbonate Secretion ◽  
Kinase C

scholarly journals Impaired mucin synthesis and bicarbonate secretion in the colon of NHE8 knockout mice

2012 ◽  
Vol 303 (3) ◽  
pp. G335-G343 ◽  
Author(s):  
Hua Xu ◽  
Bo Zhang ◽  
Jing Li ◽  
Chunhui Wang ◽  
Huacong Chen ◽  
...  
Keyword(s):  
Embryonic Stem ◽  
Physiological Role ◽  
Normal Serum ◽  
Sodium Absorption ◽  
Bicarbonate Secretion ◽  
Embryonic And Fetal Development ◽  
Sodium Hydrogen Exchanger ◽  
Important Player ◽  
Normal Serum Sodium

Sodium/hydrogen exchanger 8 (NHE8), the newest member of the SLC9 family, is expressed at the apical membrane of the epithelial cells in the intestine and the kidney. Although NHE8 has been shown to be an important player for intestinal sodium absorption early in development, its physiological role in the intestine remains unclear. Here, we successfully created a NHE8 knockout (NHE8−/−) mouse model to study the function of this transporter in the intestinal tract. Embryonic stem cells containing interrupted NHE8 gene were injected into mouse blastocyst to produce NHE8+/− chimeras. NHE8−/− mice showed no lethality during embryonic and fetal development. These mice had normal serum sodium levels and no signs of diarrhea. Apically expressed NHE2 and NHE3 were increased in the small intestine of the NHE8−/− mice in compensation. The number of goblet cells and mucin (MUC)-positive cells in the colon was reduced in NHE8−/− mice along with mucosal pH, MUC2 expression as well as downregulated in adenoma (DRA) expression. Therefore, the role of NHE8 in the intestine involves both sodium absorption and bicarbonate secretion.

Role of rho-kinase (ROCK) in tonic but not phasic contraction in the frog stomach smooth muscle

Life Sciences ◽  
2018 ◽  
Vol 198 ◽  
pp. 46-55 ◽  
Author(s):  
Leyla Sahin ◽  
Ozge Selin Cevik ◽  
Dilan Deniz Koyuncu ◽  
Kansu Buyukafsar
Keyword(s):  
Smooth Muscle ◽  
Rho Kinase ◽  
Phasic Contraction ◽  
Frog Stomach

Deoxycorticosterone-stimulated bicarbonate secretion in rabbit cortical collecting ducts: effects of luminal chloride removal and in vivo acid loading

1985 ◽  
Vol 249 (2) ◽  
pp. F205-F212 ◽  
Author(s):  
J. Garcia-Austt ◽  
D. W. Good ◽  
M. B. Burg ◽  
M. A. Knepper
Keyword(s):  
Collecting Duct ◽  
High Rate ◽  
Bicarbonate Secretion ◽  
Cortical Collecting Duct ◽  
Acid Base ◽  
Urinary Ph ◽  
Collecting Ducts ◽  
Acid Loading

To assess the role of cortical collecting duct bicarbonate secretion in the regulation of net acid excretion, we have sought to identify what factors influence the secretion rate. Net and unidirectional bicarbonate fluxes were measured in isolated perfused cortical collecting ducts from deoxycorticosterone-treated rabbits. The collecting ducts secreted bicarbonate at 11-24 pmol X mm-1 X min-1, confirming the high rate seen in earlier studies. Oral acid loading (50 mM NH4Cl drinking water) completely inhibited the net bicarbonate secretion. The bath-to-lumen flux was markedly reduced with acid loading, but the lumen-to-bath flux changed very little. In tubules from rabbits treated with deoxycorticosterone (but not NH4Cl), luminal chloride replacement with either sulfate or gluconate completely and reversibly inhibited the net bicarbonate secretion. The bath-to-lumen flux was greatly inhibited, but there was little change in the lumen-to-bath flux. We conclude: 1) High rates of bicarbonate secretion can be induced in rabbit cortical collecting ducts by chronic treatment of the animals with deoxycorticosterone. 2) When deoxycorticosterone-treated rabbits were made acidotic by oral administration of NH4Cl, the bicarbonate secretion was prevented, indicating that the systemic acid-base state of the animal may be an important factor regulating bicarbonate secretion. 3) Replacement of chloride in the lumen with sulfate inhibits bicarbonate secretion in the cortical collecting duct, an effect which may explain in part the decrease in urinary pH in response to sulfate infusions in mineralocorticoid-stimulated animals.

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