Regulation of the L-type calcium channel ?-1 subunit by chronic depolarization in the neuron-like PC12 and aortic smooth muscle A7r5 cell lines

1995 ◽  
Vol 430 (3) ◽  
pp. 323-332 ◽  
Author(s):  
Oliver Feron ◽  
Th�ophile Godfraind
Keyword(s):  
Smooth Muscle ◽  
Calcium Channel ◽  
Cell Lines ◽  
Aortic Smooth Muscle ◽  
A7r5 Cell ◽  
Chronic Depolarization

scholarly journals Co-Cultivation of Human Aortic Smooth Muscle Cells With Epicardial Adipocytes Affects Their Proliferation Rate

2014 ◽  
pp. S419-S427 ◽  
Author(s):  
J. ŽDYCHOVÁ ◽  
S. ČEJKOVÁ ◽  
I. KRÁLOVÁ LESNÁ ◽  
A. KRÁLOVÁ ◽  
J. MALUŠKOVÁ ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Cell Lines ◽  
Muscle Cells ◽  
Proliferation Rate ◽  
Bioactive Substances ◽  
Monocyte Chemoattractant Protein 1 ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle ◽  
Luminex Assay

The abnormal proliferation of vascular smooth muscle cells (VSMC) is thought to play a role in the pathogenesis of atherosclerosis. Adipocytes produce several bioactive paracrine substances that can affect the growth and migration of VSMCs. Our study focuses on the direct effect of the bioactive substances in conditioned media (CM) that was obtained by incubation with primary adipocyte-derived cell lines, including cell lines derived from both preadipocytes and from more mature cells, on the proliferation rate of human aortic smooth muscle cells (HAoSMCs). We used a Luminex assay to measure the adipokine content of the CM and showed that there was a higher concentration of monocyte chemoattractant protein-1 in renal preadipocyte-CM compared with the HAoSMC control (p<0.5). The addition of both renal preadipocyte- and epicardial adipocyte- CM resulted in the elevated production of vascular endothelial growth factor compared with the control HASoSMC CM (p<0.001). The adiponectin content in renal adipocyte-CM was increased compared to all the remaining adipocyte-CM (p<0.01). Moreover, the results showed a higher proliferation rate of HAoSMCs after co-culture with epicardial adipocyte-CM compared to the HAoSMC control (p<0.05). These results suggest that bioactive substances produced by adipocytes have a stimulatory effect on the proliferation of VSMCs.

Stable and functional expression of the calcium channel alpha 1 subunit from smooth muscle in somatic cell lines.

1992 ◽  
Vol 11 (6) ◽  
pp. 2033-2038 ◽  
Author(s):  
E. Bosse ◽  
R. Bottlender ◽  
T. Kleppisch ◽  
J. Hescheler ◽  
A. Welling ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Calcium Channel ◽  
Somatic Cell ◽  
Cell Lines ◽  
Functional Expression

Eicosapentaenoic acid inhibits vasopressin-activated Ca2+ influx and cell proliferation in rat aortic smooth muscle cell lines

1999 ◽  
Vol 379 (2-3) ◽  
pp. 199-209 ◽  
Author(s):  
Michiko Asano ◽  
Toshiaki Nakajima ◽  
Kuniaki Iwasawa ◽  
Yukari Asakura ◽  
Toshihiro Morita ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Smooth Muscle ◽  
Eicosapentaenoic Acid ◽  
Smooth Muscle Cell ◽  
Cell Lines ◽  
Muscle Cell ◽  
Aortic Smooth Muscle Cell ◽  
Aortic Smooth Muscle

Neutrophil migration through in vitro interstitial matrix

1992 ◽  
Vol 50 (1) ◽  
pp. 894-895
Author(s):  
J. Roemer ◽  
S.R. Simon
Keyword(s):  
Extracellular Matrix ◽  
Smooth Muscle ◽  
Cell Migration ◽  
Smooth Muscle Cells ◽  
Inflammatory Cell ◽  
Neutrophil Migration ◽  
Culture Conditions ◽  
Aortic Smooth Muscle ◽  
Specific Culture

We are developing an in vitro interstitial extracellular matrix (ECM) system for study of inflammatory cell migration. Falcon brand Cyclopore membrane inserts of various pore sizes are used as a support substrate for production of ECM by R22 rat aortic smooth muscle cells. Under specific culture conditions these cells produce a highly insoluble matrix consisting of typical interstitial ECM components, i.e.: types I and III collagen, elastin, proteoglycans and fibronectin.

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