Hyperosmolality rapidly reduces atrial-natriuretic-peptide-dependent cyclic guanosine monophosphate production in cultured rat inner medullary collecting duct cells

1995 ◽  
Vol 430 (1) ◽  
pp. 81-87 ◽  
Author(s):  
O. Iimura ◽  
E. Kusano ◽  
F. Ishida ◽  
S. Oono ◽  
Y. Ando ◽  
...  
Keyword(s):  
Atrial Natriuretic Peptide ◽  
Natriuretic Peptide ◽  
Collecting Duct ◽  
Cyclic Guanosine Monophosphate ◽  
Guanosine Monophosphate ◽  
Inner Medullary Collecting Duct ◽  
Duct Cells ◽  
Collecting Duct Cells ◽  
Medullary Collecting Duct ◽  
Atrial Natriuretic

Atrial natriuretic peptide and nitric oxide signaling antagonizes vasopressin-mediated water permeability in inner medullary collecting duct cells

2009 ◽  
Vol 297 (3) ◽  
pp. F693-F703 ◽  
Author(s):  
Jens Klokkers ◽  
Patrik Langehanenberg ◽  
Björn Kemper ◽  
Sebastian Kosmeier ◽  
Gert von Bally ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Plasma Membrane ◽  
Atrial Natriuretic Peptide ◽  
Natriuretic Peptide ◽  
Water Permeability ◽  
Collecting Duct ◽  
Cell Swelling ◽  
Inner Medullary Collecting Duct ◽  
Medullary Collecting Duct ◽  
Atrial Natriuretic

AVP and atrial natriuretic peptide (ANP) have opposite effects in the kidney. AVP induces antidiuresis by insertion of aquaporin-2 (AQP2) water channels into the plasma membrane of collecting duct principal cells. ANP acts as a diuretic factor. An ANP- and nitric oxide (NO)/soluble guanylate cyclase (sGC)-induced insertion of AQP2 into the plasma membrane is reported from different models. However, functional data on the insertion of AQP2 is missing. We used primary cultured inner medullary collecting duct (IMCD) cells and digital holographic microscopy, calcein-quenching measurements, and immunofluorescence and Western blotting to analyze the effects of ANP and NO donors on AQP2 phosphorylation, membrane expression, and water permeability. While AVP led to acceleration in osmotically induced swelling, ANP had no effect. However, in AVP-pretreated cells ANP significantly decreased the kinetics of cell swelling. This effect was mimicked by 8-bromo-cGMP and blunted by PKG inhibition. Stimulation of the NO/sGC pathway or direct activation of sGC with BAY 58-2667 had similar effects to ANP. In cells treated with AVP, AQP2 was predominantly localized in the plasma membrane, and after additional incubation with ANP AQP2 was mostly localized in the cytosol, indicating an increased retrieval of AQP2 from the plasma membrane by ANP. Western blot analysis showed that ANP was able to reduce AVP-induced phosphorylation of AQP2 at position S256. In conclusion, we show that the diuretic action of ANP or NO in the IMCD involves a decreased localization of AQP2 in the plasma membrane which is mediated by cGMP and PKG.

Effects of Atrial Natriuretic Peptide and Cyclic Guanosine Monophosphate on Isolated Human Myometrial Arteries Preconstricted by Endothelin-1

1995 ◽  
Vol 40 (3) ◽  
pp. 190-194 ◽  
Author(s):  
Karolina-Rasa Kublickiene ◽  
Charlotta Grunewald ◽  
Marius Kublickas ◽  
Bo Lindblom ◽  
Nils-Olov Lunell ◽  
...  
Keyword(s):  
Atrial Natriuretic Peptide ◽  
Natriuretic Peptide ◽  
Cyclic Guanosine Monophosphate ◽  
Guanosine Monophosphate ◽  
Endothelin 1 ◽  
Atrial Natriuretic
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