Effects of thermal shocks on interleukin-1 levels and heat shock protein 72 (HSP72) expression in normal human keratinocytes

1992 ◽  
Vol 284 (7) ◽  
pp. 414-417 ◽  
Author(s):  
H. Gatto ◽  
J. Viac ◽  
M. Charveron ◽  
D. Schmitt
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Interleukin 1 ◽  
Human Keratinocytes ◽  
Heat Shock Protein 72 ◽  
Normal Human Keratinocytes ◽  
Normal Human ◽  
Hsp72 Expression ◽  
Thermal Shocks

scholarly journals Regulated Overexpression of Heat Shock Protein 72 Protects Madin-Darby Canine Kidney Cells from the Detrimental Effects of High Urea Concentrations

2001 ◽  
Vol 12 (12) ◽  
pp. 2565-2571 ◽  
Author(s):  
Wolfgang Neuhofer ◽  
Karin Lugmayr ◽  
Maria-Luisa Fraek ◽  
Franz-X Beck
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Mdck Cells ◽  
Heat Shock Protein 72 ◽  
Madin Darby Canine Kidney ◽  
Lactate Dehydrogenase Activity ◽  
Hsp72 Expression ◽  
Darby Canine Kidney ◽  
Canine Kidney ◽  
Medullary Cells

ABSTRACT. Exposure of renal medullary cells to elevated extracellular NaCl concentrations is associated with increased heat shock protein 72 (HSP72) expression and improved resistance to subsequent exposure to a high urea concentration (600 mM). To establish a causal relationship between HSP72 expression and protection against high urea concentrations, HSP72 was inducibly overexpressed in Madin-Darby canine kidney (MDCK) cells, in the absence of hypertonic stress before urea exposure. For this purpose, the human stress-inducible HSP72 gene was cloned downstream from a dexamethasone (DEX)-inducible promoter in the eukaryotic expression vector pLKneo. This construct allowed robust induction of HSP72 by exposure of stably transfected MDCK cells (MDCK-LK72) to 0.1 μM DEX. Increased HSP72 abundance significantly improved survival rates after 24-h exposure of the cells to medium containing 600 mM urea (14 versus 43%). In mock-transfected or wild-type cells, DEX had no significant effect on HSP72 abundance or urea resistance. In accordance with those findings, lactate dehydrogenase activity in the supernatant was significantly reduced, compared with appropriate control samples, only in MDCK-LK72 cells overexpressing HSP72. Labeling with annexin V-FITC and propidium iodide, followed by flow cytometry, revealed that overexpression of HSP72 was associated with a reduction in the number of apoptotic-lysed cells, a concomitant retardation of apoptosis, and an increase in the number of viable cells. These data support the view that HSP72, which is very abundant in the renal inner medulla, is an important component of the defense mechanism of medullary cells against extreme concentrations of urea.

scholarly journals Molecular Mapping of Hydrogen Sulfide Targets in Normal Human Keratinocytes

2020 ◽  
Vol 21 (13) ◽  
pp. 4648
Author(s):  
Olivia Gross-Amat ◽  
Marine Guillen ◽  
Jean-Pascal Gimeno ◽  
Michel Salzet ◽  
Nicolas Lebonvallet ◽  
...  
Keyword(s):  
Molecular Mapping ◽  
Interleukin 1 ◽  
Human Keratinocytes ◽  
Thermal Waters ◽  
Cell Sheets ◽  
Experimental Conditions ◽  
Normal Human Keratinocytes ◽  
Global Mapping ◽  
Normal Human ◽  
Molecular Effects

Although sulfur-rich thermal waters have ancestrally been used in the context of dermatological conditions, a global mapping of the molecular effects exerted by H2S on human keratinocytes is still lacking. To fill this knowledge gap, we subjected cultured human keratinocytes to distinct amounts of the non-gaseous hydrogen sulfur donor NaHS. We first checked that H2S accumulated in the cytoplasm of keratinocytes under our experimental conditions andused a combination of proteomics, genomics and biochemical approaches to unravel functionally relevant H2S targets in human keratinocytes. We found that the identified targets fall into two main categories: (i) the oxidative stress response molecules superoxide dismutase 2 (SOD2), NAD(P)H quinone dehydrogenase 1 (NQO1) and culin 3 (CUL3) and (ii) the chemokines interleukin-8 (IL-8) and CXCL2. Interestingly, NaHS also stimulated the caspase-1 inflammasome pathway, leading to increased secretion of the pro-inflammatory molecule interleukin-18 (IL-18). Interestingly, the secretion of interleukin-1 beta (IL-1β) was only modestly impacted by NaHS exposure despite a significant accumulation of IL-1β pro-form. Finally, we observed that NaHS significantly hampered the growth of human keratinocyte progenitors and stem cells cultured under clonogenic conditions or as epidermal cell sheets. We conclude that H2S exerts specific molecular effects on normal human keratinocytes.

Age-related decrease in the inductability of heat shock protein 72 in normal human skin

1996 ◽  
Vol 134 (6) ◽  
pp. 1035-1058 ◽  
Author(s):  
T. MURAMATSU ◽  
M. HATOKO ◽  
H. TADA ◽  
T. SHIRAI ◽  
T. OHNISHI
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Human Skin ◽  
Heat Shock Protein 72 ◽  
Normal Human Skin ◽  
Age Related ◽  
Normal Human

scholarly journals Impaired heat shock protein 72 expression in women with polycystic ovary syndrome following a supervised exercise programme

2019 ◽  
Vol 25 (1) ◽  
pp. 73-80 ◽  
Author(s):  
Rebecca V Vince ◽  
Richard J Kirk ◽  
Myint M Aye ◽  
Stephen L Atkin ◽  
Leigh A Madden
Keyword(s):  
Heat Shock ◽  
Polycystic Ovary Syndrome ◽  
Heat Shock Protein ◽  
Polycystic Ovary ◽  
Exercise Programme ◽  
Control Group ◽  
Heat Shock Protein 72 ◽  
Ovary Syndrome ◽  
Baseline Values ◽  
Hsp72 Expression

AbstractInduction of heat shock protein expression and the heat shock (stress) response are seen in exercise. This exercise-induced response is thought protective against cellular stress through the expression of heat shock proteins. The highly inducible heat shock protein 72 (HSP72) has been shown to be expressed in a number of stress-related conditions, but not investigated in women with polycystic ovary syndrome (PCOS). Twenty-one women (10 controls, 11 with PCOS) concluded an 8-week supervised, moderate-intensity exercise programme. Monocytes and lymphocytes were analysed by flow cytometry for HSP72 expression from blood samples prior to, mid-way and at the completion of the programme. The monocyte HSP72 expression showed an increase from baseline values through mid-way (p = 0.025), and at the completion of the programme (p = 0.011) only in the control group, the PCOS group showed no significant change. This pattern was similar for lymphocyte HSP72 expression where a significant increase was found at the completion of the programme (p = 0.01) only in the control group. The magnitude of increased HSP72 expression following completion of the programme was linked to baseline values only in the control group. In conclusion, increased HSP72 expression to exercise over an 8-week period was seen in control but not in PCOS women, suggesting that there is an impairment of HSP72 expression in response to exercise in these women.

Age-related decrease in the inductability of heat shock protein 72 in normal human skin

1996 ◽  
Vol 134 (6) ◽  
pp. 1035-1058 ◽  
Author(s):  
T. MURAMATSU ◽  
M. HATOKO ◽  
H. TADA ◽  
T. SHIRAI ◽  
T. OHNISHI
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Human Skin ◽  
Heat Shock Protein 72 ◽  
Normal Human Skin ◽  
Age Related ◽  
Normal Human

scholarly journals Human keratinocytes contain mRNA indistinguishable from monocyte interleukin 1 alpha and beta mRNA. Keratinocyte epidermal cell-derived thymocyte-activating factor is identical to interleukin 1.

1986 ◽  
Vol 164 (6) ◽  
pp. 2095-2100 ◽  
Author(s):  
T S Kupper ◽  
D W Ballard ◽  
A O Chua ◽  
J S McGuire ◽  
P M Flood ◽  
...  
Keyword(s):  
Epidermal Cell ◽  
Human Peripheral Blood ◽  
Interleukin 1 ◽  
Human Keratinocytes ◽  
Blood Monocytes ◽  
Peripheral Blood Monocytes ◽  
S1 Nuclease ◽  
Normal Human Keratinocytes ◽  
Nuclease Protection Assay ◽  
Normal Human

Keratinocytes produce an IL-1 like factor termed epidermal cell-derived thymocyte-activating factor (ETAF). In this study, we show that ETAF and IL-1 are identical by the following criteria: Both normal and malignant human keratinocytes contain mRNAs identical to monocytic IL-1 alpha and IL-1 beta mRNA, as determined by an S1 nuclease protection assay; and IL-1 activity in medium conditioned by these cells can be neutralized by antibodies specific for human IL-1. The IL-1 alpha and IL-1 beta mRNAs can be identified in cultured human keratinocytes in the absence of identifiable stimulation; this basal level of mRNA can be further induced to accumulate with certain defined stimuli. Cultured normal human keratinocytes (HFKs) contain 2-4 times more IL-1 alpha than IL-1 beta mRNA; in contrast, human peripheral blood monocytes contain 10-20 times more IL-1 beta than IL-1 alpha mRNA. The IL-1 activity released by these HFK can be neutralized by an antibody that neutralizes both alpha and beta IL-1, but not by an antibody that neutralizes only IL-1 beta. While human monocytes produce a large excess of IL-1 beta after appropriate stimulation, these data suggest that IL-1 alpha is a major (and may be the predominant) form of IL-1 produced by human keratinocytes.

Correlation between Clinicopathology and Expression of Heat Shock Protein 72 in Human Hepatocellular Carcinoma

2014 ◽  
Vol 556-562 ◽  
pp. 23-26
Author(s):  
Yan Fang ◽  
Xiao Ping Wang ◽  
Huan Ping Lin ◽  
Bing Xu ◽  
Jing Gang Fang ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Lymph Node ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Human Hepatocellular Carcinoma ◽  
Tumor Infiltration ◽  
Heat Shock Protein 72 ◽  
Hepatocellular Carcinomas ◽  
Cancer Tissues ◽  
Hsp72 Expression

Heat shock protein 72 (HSP72) is highly expressed in cancer tissues. Recent studies indicate the possible roles of HSP72 in the development and progression of hepatocellular carcinomas but detailed information is still ambiguous. The aim of the study is to investigate the correlation between clinicopathology and immunolocalization of HSP72 in human hepatocellular carcinoma. Immunohistochemistry demonstrated that HSP72 expression in hepatocellular carcinomas with metastasis was significantly higher than those with non-metastasis. HSP72 expression was significantly associated with the presence of tumor infiltration, lymph node and remote metastasis.

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