High ethanol productivity from lactose by immobilized cells of Kluyveromyces fragilis and Zymomonas mobilis

1991 ◽  
Vol 7 (5) ◽  
pp. 551-556 ◽  
Author(s):  
P. Gunasekaran ◽  
N. R. Kamini
Keyword(s):  
Zymomonas Mobilis ◽  
Immobilized Cells ◽  
Ethanol Productivity ◽  
Kluyveromyces Fragilis ◽  
High Ethanol

scholarly journals Development of High-Productivity Continuous Ethanol Production using PVA-Immobilized Zymomonas mobilis in an Immobilized-Cells Fermenter

2014 ◽  
Vol 10 (2) ◽  
Author(s):  
Nurhayati Nurhayati ◽  
Chieh-Lun Cheng ◽  
Jo-Shu Chang
Keyword(s):  
Ethanol Production ◽  
Dilution Rate ◽  
Ethanol Fermentation ◽  
Zymomonas Mobilis ◽  
Immobilized Cells ◽  
Ethanol Productivity ◽  
Glucose Loading ◽  
Continuous Ethanol Fermentation ◽  
Residual Glucose ◽  
High Ethanol

Ethanol as one of renewable energy was being considered an excellent alternative clean-burning fuel to replace gasoline. Continuous ethanol fermentation systems had offered important economic advantages compared to traditional systems. Fermentation rates were significantly improved, especially when continuous fermentation was integrated with cell immobilization techniques to enrich the cells concentration in fermentor. Growing cells of Zymomonas mobilis immobilized in polyvinyl alcohol (PVA) gel beads were employed in an immobilized-cells fermentor for continuous ethanol fermentation from glucose. The glucose loading, dilution rate, and cells loading were varied in order to determine which best condition employed in obtaining both high ethanol production and low residual glucose with high dilution rate. In this study, 20 g/L, 100 g/L, 125 g/L and 150 g/L of glucose concentration and 20% (w/v), 40% (w/v) and 50% (w/v) of cells loading were employed with range of dilution rate at 0.25 to 1 h-1. The most stable production was obtained for 25 days by employing 100 g/L of glucose loading. Meanwhile, the results also exhibited that 125 g/L of glucose loading as well as 40% (w/v) of cells loading yielded high ethanol concentration, high ethanol productivity, and acceptable residual glucose at 62.97 g/L, 15.74 g/L/h and 0.16 g/L, respectively. Furthermore, the dilution rate of 4 hour with 100 g/L and 40% (w/v) of glucose and cells loading was considered as the optimum condition with ethanol production, ethanol productivity and residual glucose obtained were 49.89 g/L, 12.47 g/L/h, and 2.04 g/L, respectively. This recent study investigated ethanol inhibition as well. The present research had proved that high sugar concentration was successfully converted to ethanol. These achieved results were promising for further study.

High ethanol productivities using small Ca-alginate beads of immobilized cells of Zymomonas mobilis

1981 ◽  
Vol 3 (11) ◽  
pp. 613-618 ◽  
Author(s):  
Argyrios Margaritis ◽  
Pramod K. Bajpai ◽  
J. Blair Wallace
Keyword(s):  
Zymomonas Mobilis ◽  
Immobilized Cells ◽  
Alginate Beads ◽  
High Ethanol ◽  
Ca Alginate

scholarly journals Food Grade Ethanol Production Process of Sorghum Stem Juice Using Immobilized Cells Technique

2015 ◽  
Vol 9 (7) ◽  
pp. 8 ◽  
Author(s):  
Tri Widjaja ◽  
Ali Altway ◽  
Arief Widjaja ◽  
Umi Rofiqah ◽  
Rr Whiny Hardiyati Erlian
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Molecular Sieve ◽  
Zymomonas Mobilis ◽  
Immobilized Cells ◽  
Ethanol Yield ◽  
Continuous Fermentation ◽  
Continuous Process ◽  
Pichia Stipitis ◽  
Batch Process ◽  
Food Grade

One form of economic development efforts for waste utilization in rural communities is to utilize stem sorghum to produce food grade ethanol. Sorghum stem juice with 150 g/L of sugar concentration was fermented using conventional batch process and cell immobilization continuous process with K-carrageenan as a supporting matrix. The microorganism used was Mutated Zymomonas Mobilis to be compared with a mixture of Saccharomyces Cerevisiae and Pichia Stipitis, and a mixture of Mutated Zymomonas Mobilis and Pichia Stipitis. Ethanol in the broth, result of fermentation process, was separated in packed distillation column. Distilate of the column, still contain water and other impurities, was flown into molecular sieve for dehydration and activated carbon adsorption column to remove the other impurities to meet food grade ethanol specification. The packing used in distillation process was steel wool. For batch fermentation, the fermentation using a combination of Saccharomyces Cerevisiae and Pichia Stipitis produced the best ethanol with 12.07% of concentration, where the yield and the productivity were 63.49%, and 1.06 g/L.h, respectively. And for continuous fermentation, the best ethanol with 9.02% of concentration, where the yield and the productivity were 47.42% and 174.27 g/L.h, respectively, is obtained from fermentation using a combination of Saccharomyces Cerevisiae and Pichia Stipitis also. Fermentation using combination microorganism of Saccharomyces Cerevisiae and Pichia Stipitis produced higher concentration of ethanol, yield, and productivity than other microorganisms. Distillation, molecular sieve dehydration and adsorption process is quite successful in generating sufficient levels of ethanol with relatively low amount of impurities.

Production of organic acids by periplasmic enzymes present in free and immobilized cells of Zymomonas mobilis

2012 ◽  
Vol 40 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Eloane Malvessi ◽  
Sabrina Carra ◽  
Flávia Cristina Pasquali ◽  
Denise Bizarro Kern ◽  
Mauricio Moura da Silveira ◽  
...  
Keyword(s):  
Organic Acids ◽  
Zymomonas Mobilis ◽  
Immobilized Cells

Production of Ethanol as a Renewable Energy by Extractive Fermentation

2014 ◽  
Vol 493 ◽  
pp. 300-305 ◽  
Author(s):  
Tri Widjaja ◽  
Ali Altway ◽  
Ayu Ratna Permanasari ◽  
Setiyo Gunawan
Keyword(s):  
Immobilized Cells ◽  
Continuous Fermentation ◽  
Packed Bed ◽  
Extraction Process ◽  
Amyl Alcohol ◽  
Product Inhibition ◽  
Ethanol Productivity ◽  
Extractive Fermentation ◽  
Packed Bed Bioreactor ◽  
Recycle Ratio

One issue with batch fermentation is that product inhibition causes low yields and ethanol productivity. The objective of this study was to increase the yield and ethanol productivity via continuous fermentation in a packed bed bioreactor with both an integrated extraction process and recycling of the raffinate into the fermenter. Molasses was used as the feedstock, and the immobilized cells were supported by ĸ-carrageenan. This process used n-amyl alcohol, 1-octanol, and 1-dodecanol as solvents. The yield and ethanol productivity increased from 8.79% to 20.03% and 34.54 g/L·h to 118.16 g/L·h for experiments using n-amyl alcohol, 9.05% to 12.67% and 35.59 g/L·h to 74.71 g/L·h, for 1-dodecanol, 8.89% to 13.45% and 34.93 g/L·h to 84.62 g/L·h, for1-octanol by increasing recycle ratio from 0 to 0.5. Based on these results, n-amyl alcohol was the best solvent for the extractive fermentation process.

Two-reactor, continuous culture fermentation for fuel ethanol production from lignocellulosic acid hydrolysate using Zymomonas mobilis and Scheffersomyces stipitis

RSC Advances ◽  
2014 ◽  
Vol 4 (69) ◽  
pp. 36412-36418 ◽  
Author(s):  
Gaurav Chaudhary ◽  
Sanjoy Ghosh
Keyword(s):  
Ethanol Production ◽  
Continuous Culture ◽  
Zymomonas Mobilis ◽  
Fuel Ethanol ◽  
Ethanol Productivity ◽  
Scheffersomyces Stipitis ◽  
Acid Hydrolysate

Multistage ethanol production from lignocellulosic acid hydrolysate using two different microorganisms in two reactors enhances the utilization of all sugars (pentose and hexose), with higher ethanol productivity.

Ethanol fermentation of mahula (Madhuca latifolia) flowers using free and immobilized bacteria Zymomonas mobilis MTCC 92

Biologia ◽  
2010 ◽  
Vol 65 (3) ◽  
Author(s):  
Shuvashish Behera ◽  
Rama Mohanty ◽  
Ramesh Ray
Keyword(s):  
Calcium Alginate ◽  
Zymomonas Mobilis ◽  
Immobilized Cells ◽  
Dry Weight ◽  
Weight Basis ◽  
Deciduous Tree ◽  
Madhuca Latifolia ◽  
Australian Continent ◽  
Free Cells ◽  
Alginate Matrix

AbstractMahula (Madhuca latifolia L.) is a deciduous tree commonly found in the tropical rain forests of Asian and Australian continent. Corolla, the edible part of its flowers, is rich in fermentable sugar (37 ± 0.23%; on dry weight basis). Batch fermentation of mahula flowers was carried out using Zymomonas mobilis MTCC 92 free cells and cells immobilized in calcium alginate matrix. The ethanol productions were 122.9 ± 0.972 and 134.6 ± 0.104 g/kg flowers on dry weight basis using free and immobilized cells, respectively, after 96 h of fermentation, which showed that cells entrapped in calcium alginate matrix yielded 8.7% more ethanol than free cells. Further, the immobilized cells were physiologically active up to three more cycles of fermentation producing 132.7 ± 0.095, 130.5 ± 0.09 and 128.7 ± 0.056 g ethanol per kg flower in first, second and third cycle, respectively.

Sign in / Sign up

Export Citation Format

Share Document