Trans-nuclear action of the nit-2 regulatory gene product and study of two additional nitrogen control genes in Neurospora crassa

1983 ◽  
Vol 7 (1) ◽  
pp. 51-56 ◽  
Author(s):  
John A. A. Chambers ◽  
Sherri M. Griffon ◽  
George A. Marzluf
Keyword(s):  
Neurospora Crassa ◽  
Gene Product ◽  
Regulatory Gene ◽  
Nitrogen Control ◽  
Additional Nitrogen

scholarly journals Isolation of a gene that down-regulates nitrate assimilation and influences another regulatory gene in the same system.

1989 ◽  
Vol 9 (9) ◽  
pp. 4113-4117 ◽  
Author(s):  
G J Sorger ◽  
D Brown ◽  
M Farzannejad ◽  
A Guerra ◽  
M Jonathan ◽  
...  
Keyword(s):  
Neurospora Crassa ◽  
Gene Product ◽  
Regulatory Gene ◽  
Nitrate Assimilation ◽  
Gene Products ◽  
Specific Pathway

Glutamine is the preferred source of nitrogen of Neurospora crassa. In its presence and that of the gene product of MS5 (nmr-1), the fungus represses the assimilation of less preferred forms of nitrogen, such as nitrate. In the absence of glutamine and the presence of the product of gene nit-2, less preferred forms of nitrogen are assimilated as long as a specific pathway for their assimilation is induced. We report here the isolation, from a cosmid bank, of a gene that complements MS5 and can also complement nit-2. We speculate that this result suggests an interaction between the MS5 and nit-2 gene products and that this is important in the regulation of nitrate assimilation.

Isolation of a gene that down-regulates nitrate assimilation and influences another regulatory gene in the same system

1989 ◽  
Vol 9 (9) ◽  
pp. 4113-4117
Author(s):  
G J Sorger ◽  
D Brown ◽  
M Farzannejad ◽  
A Guerra ◽  
M Jonathan ◽  
...  
Keyword(s):  
Neurospora Crassa ◽  
Gene Product ◽  
Regulatory Gene ◽  
Nitrate Assimilation ◽  
Gene Products ◽  
Specific Pathway

Glutamine is the preferred source of nitrogen of Neurospora crassa. In its presence and that of the gene product of MS5 (nmr-1), the fungus represses the assimilation of less preferred forms of nitrogen, such as nitrate. In the absence of glutamine and the presence of the product of gene nit-2, less preferred forms of nitrogen are assimilated as long as a specific pathway for their assimilation is induced. We report here the isolation, from a cosmid bank, of a gene that complements MS5 and can also complement nit-2. We speculate that this result suggests an interaction between the MS5 and nit-2 gene products and that this is important in the regulation of nitrate assimilation.

scholarly journals GENETICS OF ARGININE BIOSYNTHESIS IN NEUROSPORA CRASSA

Genetics ◽  
1979 ◽  
Vol 93 (3) ◽  
pp. 557-575
Author(s):  
Rowland H Davis
Keyword(s):  
Neurospora Crassa ◽  
Gene Product ◽  
Unit Interval ◽  
Starting Material ◽  
Limited Extent ◽  
Arginine Biosynthesis ◽  
Regulatory Mutants ◽  
Intragenic Complementation ◽  
Translocation Breakpoints ◽  
Bifunctional Gene

ABSTRACT A large number of arginine-requiring mutants of Neurospora was isolated, using a strain already partially impaired in an enzyme of the pathway. Among the mutants, all previously described loci, except one, were represented, and several new loci were defined and mapped. Four groups of mutants were of particular interest. First, thc large group of arg-6 mutants, when tested for intragenic complementation, suggested a bifunctional gene, possibly controlling two steps in ornithine synthesis. This is consistent with the limited enzymic information about this locus. Second, the arg-13 locus was represented by 14 new mutants. All five tested were quite leaky. suggesting that the function controlled by this gene can be rarried out to a limited extent spontaneously or by another gene product. Third, a new locus, arg-14, was defined. It controls a step in ornithine synthesis. It lies in a 1 to 2 map-unit interval between arg-2 and pyr-3 on LG IVR, as shown by mapping in relation tG translocation breakpoints. Fourth, a second new locus whose mutants render the partial mutation in starting material auxotrophic was defined and mapped near the centromere of LG VIL. These new mutants are unable to derepress enzymes of the pathway and may qualify as regulatory mutants.

scholarly journals Regulatory gene product of the Ah locus. Characterization of the cytosolic inducer-receptor complex and evidence for its nuclear translocation.

1979 ◽  
Vol 254 (22) ◽  
pp. 11636-11648 ◽  
Author(s):  
A.B. Okey ◽  
G.P. Bondy ◽  
M.E. Mason ◽  
G.F. Kahl ◽  
H.J. Eisen ◽  
...  
Keyword(s):  
Gene Product ◽  
Nuclear Translocation ◽  
Regulatory Gene ◽  
Receptor Complex
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