A monoclonal antibody which detects a polymorphic la antigenic determinant reacts with purified ? polypeptide chain

1981 ◽  
Vol 13 (4) ◽  
pp. 347-350 ◽  
Author(s):  
W. Robert McMaster
Keyword(s):  
Monoclonal Antibody ◽  
Antigenic Determinant ◽  
Polypeptide Chain

scholarly journals Fixation of Thy-1 in nervous tissue for immunohistochemistry: a quantitative assessment of the effect of different fixation conditions upon retention of antigenicity and the cross-linking of Thy-1.

1983 ◽  
Vol 31 (2) ◽  
pp. 263-274 ◽  
Author(s):  
R J Morris ◽  
P C Barber
Keyword(s):  
Monoclonal Antibody ◽  
Antigenic Determinant ◽  
Sodium Dodecyl ◽  
Rabbit Antiserum ◽  
Sodium Deoxycholate ◽  
Antigenic Determinants ◽  
Cross Linking ◽  
Cell Surface Antigens ◽  
Vascular Perfusion ◽  
Fixed Tissue

It has proved difficult to obtain good immunohistochemical localization of cell surface antigens in nerve for a number of reasons, prominent among which are problems of fixing this class of molecule without destroying their antigenicity. In the course of developing a fixation procedure suitable for one such antigen. Thy-1, we have quantitatively assessed the effect of different fixation parameters upon the retention of Thy-1 antigenicity and upon the extent of cross-linking of the antigen in the tissue. The former was measured using radioimmunoassays adapted for membrane antigens in fixed tissue, the latter by measuring the proportion of antigen rendered insoluble to the detergent, sodium deoxycholate, and by examining the size of the antigen on sodium dodecyl sulfate--polyacrylamide gels. These approaches demonstrated that minor modifications of the standard vascular perfusion fixation of brain, using both glutaraldehyde and paraformaldehyde, were sufficient to fix the Thy-1 molecule, and at the same time substantially spare its antigenicity. In this study we measured Thy-1 using both a conventional rabbit antiserum and a mouse monoclonal antibody to the Thy-1.1 antigenic determinant. The multiple antigenic determinants recognized by the rabbit antibodies were cumulatively more resistant to fixation than the single antigenic determinant recognized by the monoclonal antibody.

New HLA antigenic determinant shared by A2 and a subtype of Bw16 molecules detected by a monoclonal antibody

1983 ◽  
Vol 7 (1) ◽  
pp. 17-23 ◽  
Author(s):  
P. Richiardi ◽  
T. Crepaldi ◽  
F. Malavasi ◽  
A. Carbonara
Keyword(s):  
Monoclonal Antibody ◽  
Antigenic Determinant

Basket cell development in the normal and hypothyroid rat cerebellar cortex studied with a monoclonal anti-neurofilament antibody

1985 ◽  
Vol 63 (6) ◽  
pp. 564-576 ◽  
Author(s):  
Nicole Leclerc ◽  
Claude Gravel ◽  
Andrius Plioplys ◽  
Richard Hawkes
Keyword(s):  
Monoclonal Antibody ◽  
Cell Differentiation ◽  
Purkinje Cell ◽  
Cerebellar Cortex ◽  
Antigenic Determinant ◽  
Cell Development ◽  
Cell Maturation ◽  
Basket Cell ◽  
Neurofilament Protein ◽  
Basket Cells

We have used a monoclonal antibody against an antigenic determinant of the 210-kdalton neurofilament protein to study basket cell maturation in rat cerebellar cortex. Neurofilament immunoreactivity first appears in basket cells at postnatal day 12 and mature axonal "pinceaux" are present at postnatal day 17. There are large differences in the rate of maturation from lobe to lobe which do not fully correspond to the rate of Purkinje cell differentiation. In hypothyroid rats the expression of the neurofilament antigen by basket cells is almost completely suppressed.

A monoclonal antibody that recognizes an antigenic determinant shared by HLA A2 and B17

1980 ◽  
Vol 1 (2) ◽  
pp. 121-129 ◽  
Author(s):  
Andrew J. McMichael ◽  
Peter Parham ◽  
Nigel Rust ◽  
Frances Brodsky
Keyword(s):  
Monoclonal Antibody ◽  
Antigenic Determinant

Species specificity of a monoclonal antibody produced to Naegleria fowleri and partial characterization of its antigenic determinant

2000 ◽  
Vol 86 (8) ◽  
pp. 634-641 ◽  
Author(s):  
Fabienne L. Réveiller ◽  
Francine Marciano-Cabral ◽  
Pierre Pernin ◽  
Pierre-André Cabanes ◽  
Stéphane Legastelois
Keyword(s):  
Monoclonal Antibody ◽  
Antigenic Determinant ◽  
Species Specificity ◽  
Partial Characterization ◽  
Naegleria Fowleri ◽  
Nægleria Fowleri

FAB CID-MS/MS characterization of tetrasaccharide tri- and tetrasulfate derived from the antigenic determinant recognized by the anti-chondroitin sulfate monoclonal antibody MO-225

1995 ◽  
Vol 12 (3) ◽  
pp. 282-289 ◽  
Author(s):  
Tadashi II ◽  
Masayuki Kubota ◽  
Takashi Hirano ◽  
Mamoru Ohashi ◽  
Keiichi Yoshida ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Chondroitin Sulfate ◽  
Antigenic Determinant

scholarly journals Antigenic determinant and interspecies cross-reactivity of a monoclonal antibody to poly(ADP-ribose) synthetase

FEBS Letters ◽  
1985 ◽  
Vol 182 (2) ◽  
pp. 393-397 ◽  
Author(s):  
Isamu Kameshita ◽  
Hiroshi Yamamoto ◽  
Shigeyoshi Fujimoto ◽  
Yutaka Shizuta
Keyword(s):  
Monoclonal Antibody ◽  
Antigenic Determinant ◽  
Cross Reactivity
Sign in / Sign up

Export Citation Format

Share Document