Monoclonal antibodies defining mouse tissue antigens encoded by the H-2 region

1987 ◽  
Vol 25 (2) ◽  
pp. 136-139 ◽  
Author(s):  
Kim J. Hasenkrug ◽  
Joan M. Cory ◽  
Jack H. Stimpfling
Keyword(s):  
Monoclonal Antibodies ◽  
Mouse Tissue ◽  
Tissue Antigens

Cross reactions between Plasmodium falciparum and mammalian tissue antigens detected by monoclonal antibodies

1984 ◽  
Vol 78 (1) ◽  
pp. 75-76 ◽  
Author(s):  
G. Daniel Ribeiro ◽  
J. Kalil ◽  
L. Monjour ◽  
C. Alfred ◽  
I. Ploton ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Monoclonal Antibodies ◽  
Mammalian Tissue ◽  
Cross Reactions ◽  
Tissue Antigens

scholarly journals Reaction of Human Monoclonal Antibodies to SARS-CoV-2 Proteins With Tissue Antigens: Implications for Autoimmune Diseases

2021 ◽  
Vol 11 ◽  
Author(s):  
Aristo Vojdani ◽  
Elroy Vojdani ◽  
Datis Kharrazian
Keyword(s):  
Monoclonal Antibodies ◽  
Autoimmune Diseases ◽  
Human Tissue ◽  
Molecular Mimicry ◽  
Disease Process ◽  
Cross Reactivity ◽  
Immune Reactivity ◽  
Human Tissues ◽  
Human Monoclonal Antibodies ◽  
Tissue Antigens

We sought to determine whether immune reactivity occurs between anti-SARS-CoV-2 protein antibodies and human tissue antigens, and whether molecular mimicry between COVID-19 viral proteins and human tissues could be the cause. We applied both human monoclonal anti-SARS-Cov-2 antibodies (spike protein, nucleoprotein) and rabbit polyclonal anti-SARS-Cov-2 antibodies (envelope protein, membrane protein) to 55 different tissue antigens. We found that SARS-CoV-2 antibodies had reactions with 28 out of 55 tissue antigens, representing a diversity of tissue groups that included barrier proteins, gastrointestinal, thyroid and neural tissues, and more. We also did selective epitope mapping using BLAST and showed similarities and homology between spike, nucleoprotein, and many other SARS-CoV-2 proteins with the human tissue antigens mitochondria M2, F-actin and TPO. This extensive immune cross-reactivity between SARS-CoV-2 antibodies and different antigen groups may play a role in the multi-system disease process of COVID-19, influence the severity of the disease, precipitate the onset of autoimmunity in susceptible subgroups, and potentially exacerbate autoimmunity in subjects that have pre-existing autoimmune diseases. Very recently, human monoclonal antibodies were approved for use on patients with COVID-19. The human monoclonal antibodies used in this study are almost identical with these approved antibodies. Thus, our results can establish the potential risk for autoimmunity and multi-system disorders with COVID-19 that may come from cross-reactivity between our own human tissues and this dreaded virus, and thus ensure that the badly-needed vaccines and treatments being developed for it are truly safe to use against this disease.

scholarly journals Light and electron microscopic localization of the alpha 1-chain and the E1 and E8 domains of laminin-1 in mouse kidney using monoclonal antibodies to establish the orientation of laminin-1 within basement membranes.

1995 ◽  
Vol 43 (7) ◽  
pp. 675-680 ◽  
Author(s):  
N Miosge ◽  
E Günther ◽  
E Heyder ◽  
B Manshausen ◽  
R Herken
Keyword(s):  
Monoclonal Antibodies ◽  
Proximal Tubule ◽  
Distal Tubule ◽  
Distinct Pattern ◽  
Mouse Kidney ◽  
Basement Membranes ◽  
Mouse Tissue ◽  
Electron Microscopic ◽  
Laminin 1

To localize the different domains of the laminin-1 molecule in tissues and gain insight into their in vivo relevance, we raised rat anti-mouse monoclonal antibodies (MAbs) against the entire molecule. Then we tested eight of the 20 clones producing anti-laminin-1 MAbs to specify their reactivity towards the alpha 1-, beta 1-, and gamma 1-chains and the elastase-cleaved fragments of the laminin-1 molecule. We found three MAbs with high titers in ELISA that showed good reactivity in embedded tissue. One of these reacted specifically against the E1 fragment, one against the E8 fragment, and one MAb detected the alpha 1-chain of laminin-1 but not the beta 1- or gamma 1-chain. All three MAbs are useful for light immunohistochemical investigations on cryosections and on paraffin-embedded material, and for ultrastructural localization of laminin-1 in LR Gold-embedded mouse tissue. Antibody staining of the E1 and E8 domains of laminin-1 revealed distinct localization of the molecule in the proximal tubule basement membranes of mouse kidney. The short arms (E1) of the laminin-1 molecule are predominantly located in the lamina lucida and the long arms (E8) are oriented towards the lamina fibroreticularis. Therefore, both MAbs are useful for studies of the orientation of the laminin-1 molecule in basement membranes. The distal tubule basement membranes did not show any distinct pattern of laminin-1 distribution. In general, the distal tubules showed the strongest reactions over the entire width of the basement membrane for all three MAbs. In contrast, the proximal tubule basement membranes showed somewhat weaker reactivity but a distinct pattern of laminin-1 distribution, with the E1 fragments oriented towards the adjacent epithelial cell surface.

scholarly journals Monoclonal antibodies as probes of the distribution of ZP-2, the major sulfated glycoprotein of the murine zona pellucida.

1984 ◽  
Vol 98 (3) ◽  
pp. 795-800 ◽  
Author(s):  
I J East ◽  
J Dean
Keyword(s):  
Extracellular Matrix ◽  
Monoclonal Antibodies ◽  
Zona Pellucida ◽  
Binding Sites ◽  
Antigenic Site ◽  
Matrix Proteins ◽  
Mouse Tissue ◽  
Cell Embryo ◽  
Embryo Stage ◽  
Mouse Egg

Three sulfated glycoproteins (ZP-1, ZP-2, and ZP-3) make up the zona pellucida, an extracellular glycocalyx that surrounds mouse oocytes. We have produced five monoclonal antibodies specific to the zona. All five immunoprecipitated ZP-2, and in addition, two of the antibodies immunoprecipitated ZP-3. This suggests the presence of either a common antigenic site or one made up in part by each of the two glycoproteins. The monoclonal antibodies bound to approximately 1.3 X 10(8) binding sites per ovulated mouse egg which represents 2% of the total number of ZP-2 molecules present in the zona. ZP-2 appeared to be present throughout the zona and indirect immunofluorescence revealed a fibrous pattern with no evidence of localization. Furthermore, this pattern of distribution, which was identical for all five monoclones, remained constant after fertilization at the two-cell embryo stage. Laser photobleaching demonstrated that ZP-2 is stably integrated in the extracellular matrix of the zona pellucida. No mouse tissue other than the ovary contained ZP-2 and ZP-2 is antigenically distinct from other previously described extracellular matrix proteins.

Immunoelectron microscopic demonstration of tissue antigens with monoclonal antibodies

1984 ◽  
Vol 46 (1) ◽  
pp. 1-12 ◽  
Author(s):  
M. L. Hansmann ◽  
H. J. Radzun ◽  
E. Kaiserling ◽  
M. R. Parwaresch
Keyword(s):  
Monoclonal Antibodies ◽  
Microscopic Demonstration ◽  
Tissue Antigens

scholarly journals A novel modification of the avidin-biotin complex method for immunohistochemical studies of transgenic mice with murine monoclonal antibodies.

1992 ◽  
Vol 40 (9) ◽  
pp. 1319-1328 ◽  
Author(s):  
K M Fung ◽  
A Messing ◽  
V M Lee ◽  
J Q Trojanowski
Keyword(s):  
Monoclonal Antibodies ◽  
Transgenic Mice ◽  
Detection System ◽  
Immunohistochemical Method ◽  
Mouse Tissue ◽  
Mouse Serum ◽  
Complex Method ◽  
Mouse Tissues ◽  
Secondary Antibodies ◽  
Murine Monoclonal Antibodies

When mouse tissues are probed with murine monoclonal antibodies (MAb) by indirect immunohistochemistry, the secondary antibody detects tissue-bound MAb and irrelevant, endogenous mouse immunoglobulins. The latter are a source of confounding background, especially in diseased tissues. To circumvent this problem, we generated complexes of primary MAb and biotinylated secondary antibodies in vitro for use as antigen-specific probes. After blocking free binding sites in the complexed secondary antibodies with normal mouse serum, the complexes were applied to mouse tissue sections and tissue-bound complexes were visualized with an avidin-biotin detection system. Complexes formed with 12 different rat or mouse MAb were used to probe sections of normal mice, tumor-bearing transgenic mice, and mice with tumor xenografts. The staining patterns produced by these probes reflected the specificity of the MAb in the complexes, and the labeling of irrelevant, endogenous mouse immunoglobulins was reduced substantially. This novel, indirect immunohistochemical method can be exploited to study normal and diseased mouse tissues using a variety of murine MAb.

The identification of soluble adult antigen on the tegumental surface of juvenile Fasciola hepatica

Parasitology ◽  
1978 ◽  
Vol 77 (3) ◽  
pp. 325-332 ◽  
Author(s):  
C. E. Bennett
Keyword(s):  
Indirect Immunofluorescence ◽  
Post Infection ◽  
Fasciola Hepatica ◽  
Mouse Tissue ◽  
Surface Coat ◽  
Soluble Extract ◽  
Hepatic Parenchyma ◽  
Tegumental Surface ◽  
Tissue Antigens ◽  
Juvenile Stages

SummaryAn antiserum was raised in rabbits against a soluble extract of fresh homogenized adult Fasciola hepatica of rat origin and was then absorbed with rat and mouse tissue antigens. This antiserum reacted specifically with the surface coat of adult flukes, of both rat and mouse origin, by indirect immunofluorescence to show the detail of surface spines. When tested against juvenile stages recovered from mice the reaction was positive with all but the earliest hepatic parenchyma stages. No reaction was present on the tegumental surface of newly excysted juveniles or stages 1 or 2 days post-infection (p.i.) whether recovered from the peritoneal cavity or the hepatic parenchyma.

scholarly journals Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.

1995 ◽  
Vol 43 (3) ◽  
pp. 313-320 ◽  
Author(s):  
J L Whiteland ◽  
S M Nicholls ◽  
C Shimeld ◽  
D L Easty ◽  
N A Williams ◽  
...  
Keyword(s):  
T Cells ◽  
Monoclonal Antibodies ◽  
Mhc Class Ii ◽  
Immune Cell ◽  
Immunohistochemical Localization ◽  
T Cell Subsets ◽  
Mouse Tissue ◽  
Paraffin Sections ◽  
Mouse Tissues ◽  
Mhc Class Ii Antigens

We describe a method for immunohistochemical localization of T-cells, CD4+ T-cells, CD8+ T-cells, B-cells, activated lymphocytes, major histocompatibility complex (MHC) class II antigens, macrophages, dendritic cells, and granulocytes in rat and mouse tissue fixed in periodate-lysine-paraformaldehyde (PLP) and embedded in paraffin. Rat and mouse spleen and eyes were fixed in PLP for 18-24 hr, rapidly dehydrated, infiltrated under vacuum with paraffin at 54 degrees C, sectioned, and stained with appropriate monoclonal antibodies (MAbs). Sections of PLP-fixed, paraffin-embedded spleen were compared with acetone-fixed frozen spleen sections with respect to morphology and staining quality. Nine of 10 MAbs to rat antigens and eight of nine MAbs to mouse antigens stained paraffin sections equally or more intensely than frozen sections. The two MAbs that showed weaker staining still gave good staining on paraffin sections. Paraffin-embedded rat and mouse eyes were easier to section serially than frozen eyes, showed superior morphology, and individually stained cells were readily identified. Therefore, a combination of PLP fixation and low-temperature paraffin embedding permits detection of the major types of immune cell in rat and mouse tissues while maintaining good morphology, particularly in diseased, damaged, or delicate tissues.

Glucuronyl 3-sulfate occurs exclusively on distal unmyelinated terminals of selected sensory neurons in the peripheral nervous system

1995 ◽  
Vol 53 ◽  
pp. 958-959
Author(s):  
S.S. Spicer ◽  
B.A. Schulte
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Cerebral Cortex ◽  
Peripheral Nervous System ◽  
Sensory Neurons ◽  
Sensory Nerves ◽  
Tissue Antigens ◽  
The Central Nervous System ◽  
The Brain ◽  
Leu 7

Generation of monoclonal antibodies (MAbs) against tissue antigens has yielded several (VC1.1, HNK- 1, L2, 4F4 and anti-leu 7) which recognize the unique sugar epitope, glucuronyl 3-sulfate (Glc A3- SO4). In the central nervous system, these MAbs have demonstrated Glc A3-SO4 at the surface of neurons in the cerebral cortex, the cerebellum, the retina and other widespread regions of the brain.Here we describe the distribution of Glc A3-SO4 in the peripheral nervous system as determined by immunostaining with a MAb (VC 1.1) developed against antigen in the cat visual cortex. Outside the central nervous system, immunoreactivity was observed only in peripheral terminals of selected sensory nerves conducting transduction signals for touch, hearing, balance and taste. On the glassy membrane of the sinus hair in murine nasal skin, just deep to the ringwurt, VC 1.1 delineated an intensely stained, plaque-like area (Fig. 1). This previously unrecognized structure of the nasal vibrissae presumably serves as a tactile end organ and to our knowledge is not demonstrable by means other than its selective immunopositivity with VC1.1 and its appearance as a densely fibrillar area in H&E stained sections.

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