Sequence determination of a transcribed rabbit class II gene with homology to HLA-DQ ?

1987 ◽  
Vol 25 (2) ◽  
pp. 104-109 ◽  
Author(s):  
Christian LeGuern ◽  
Jocelyn D. Weissman ◽  
Patrice N. Marcher ◽  
Evelyne Jouvin-Marcher ◽  
Anne Laverri�re ◽  
...  
Keyword(s):  
Class Ii ◽  
Sequence Determination ◽  
Hla Dq ◽  
Rabbit Class

Structure of the class II enzyme of human liver alcohol dehydrogenase: combined complementary DNA and protein sequence determination of the .pi. subunit

Biochemistry ◽  
1987 ◽  
Vol 26 (7) ◽  
pp. 1926-1932 ◽  
Author(s):  
Jan Olov Höög ◽  
Hedvig Von Bahr-Lindstroem ◽  
Lars Olof Heden ◽  
Barton Holmquist ◽  
Kerstin Larsson ◽  
...  
Keyword(s):  
Alcohol Dehydrogenase ◽  
Protein Sequence ◽  
Human Liver ◽  
Class Ii ◽  
Sequence Determination ◽  
Complementary Dna ◽  
Liver Alcohol Dehydrogenase

Isolation and sequence determination of porcine class II DRB alleles amplified by PCR

2009 ◽  
Vol 25 ◽  
pp. 73-75 ◽  
Author(s):  
D. I. Våge ◽  
I. Olsaker ◽  
F. Lingaas ◽  
O. Lie
Keyword(s):  
Class Ii ◽  
Sequence Determination

Isolation and sequence determination of porcine class II DRB alleles amplified by PCR

2009 ◽  
Vol 25 (2) ◽  
pp. 73-75 ◽  
Author(s):  
D I Våge ◽  
I Olsaker ◽  
F Lingaas ◽  
Ø Lie
Keyword(s):  
Class Ii ◽  
Sequence Determination

MICROBIAL COMMUNITY DURING BIOREMEDIATION EXPERIMENTAL ON OIL SPILL IN COASTAL OF PARI ISLAND

2011 ◽  
Vol 3 (1) ◽  
Author(s):  
Lies Indah Sutiknowati
Keyword(s):  
Oil Spill ◽  
Deoxyribonucleic Acid ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Gas Chromatography Mass Spectrometry ◽  
Oil Degradation ◽  
Sequence Determination ◽  
Blast Search ◽  
Degrading Bacteria ◽  
Gradient Gel Electrophoresis

There is an information how to identify hydrocarbon degrading bacteria for bioremediation of marine oil spill. We have Bioremediation treatment for degradation of oil spill on Pari island and need two kind of experiment there are tanks experiment (sampling 0 to 90 days) and semi enclosed system (sampling 0 to 150 days). Biostimulation with nutrients (N and P) was done to analyze biodegradation of hydrocarbon compounds. Experiment design using fertilizer Super IB and Linstar will stimulate bacteria can degrade oil, n-alkane, and alkane as poly aromatic hydrocarbon. The bacteria communities were monitored and analyzed by Denaturing Gradient Gel Electrophoresis (DGGE) and Clone Library; oil chemistry was analyzed by Gas Chromatography Mass Spectrometry (GCMS). DNA (deoxyribonucleic acid) was extracted from colonies of bacteria and sequence determination of the 16S rDNA was amplified by primers U515f and U1492r. Strains had been sequence and had similarity about 90-99% to their closest taxa by homology Blast search and few of them suspected as new species. The results showed that fertilizers gave a significant effect on alkane, PAH and oil degradation in tanks experiment but not in the field test. Dominant of the specific bacteria on this experiment were Alcanivorax, Marinobacter and Prosthecochloris. Keywords: Bioremediation, Biostimulation, DGGE, PAH, Pari Island

scholarly journals HLA-DQB1 codon 57 is critical for peptide binding and recognition.

1996 ◽  
Vol 183 (3) ◽  
pp. 1253-1258 ◽  
Author(s):  
W W Kwok ◽  
M E Domeier ◽  
M L Johnson ◽  
G T Nepom ◽  
D M Koelle
Keyword(s):  
Amino Acid ◽  
T Cell ◽  
Cell Clone ◽  
Peptide Binding ◽  
Class Ii ◽  
Binding Studies ◽  
Peptide Complex ◽  
Mhc Molecules ◽  
Hla Dq ◽  
T Cell Clone

The association of specific HLA-DQ alleles with autoimmunity is correlated with discrete polymorphisms in the HLA-DQ sequence that are localized within sites suitable for peptide recognition. The polymorphism at residue 57 of the DQB1 polypeptide is of particular interest since it may play a major structural role in the formation of a salt bridge structure at one end of the peptide-binding cleft of the DQ molecules. This polymorphism at residue 57 is a recurrent feature of HLA-DQ evolution, occurring in multiple distinct allelic families, which implies a functional selection for maintaining variation at this position in the class II molecule. We directly tested the amino acid polymorphism at this site as a determinant for peptide binding and for antigen-specific T cell stimulation. We found that a single Ala-->Asp amino acid 57 substitution in an HLA-DQ3.2 molecule regulated binding of an HSV-2 VP-16-derived peptide. A complementary single-residue substitution in the peptide abolished its binding to DQ3.2 and converted it to a peptide that can bind to DQ3.1 and DQ3.3 Asp-57-positive MHC molecules. These binding studies were paralleled by specific T cell recognition of the class II-peptide complex, in which the substituted peptide abolished T cell reactivity, which was directed to the DQ3.2-peptide complex, whereas the same T cell clone recognized the substituted peptide presented by DQ3.3, a class II restriction element differing from DQ3.2 only at residue 57. This structural and functional complementarity for residue 57 and a specific peptide residue identifies this interaction as a key controlling determinant of restricted recognition in HLA-DQ-specific immune response.

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