The Adh in Drosophila: Chromosomal location and restriction analysis in species with different phylogenetic relationships

Chromosoma ◽  
1991 ◽  
Vol 100 (5) ◽  
pp. 315-322 ◽  
Author(s):  
N. Visa ◽  
G. Marfany ◽  
L. Vilageliu ◽  
R. Albalat ◽  
S. Atrian ◽  
...  
Keyword(s):  
Phylogenetic Relationships ◽  
Chromosomal Location ◽  
Restriction Analysis

Architecture of Radiolitid Rudist (Mollusca) Shell-Wall Structures and its Phylogenetic Implications

1975 ◽  
Vol 33 ◽  
pp. 686-687
Author(s):  
David H. Sturm ◽  
Bob F. Perkins
Keyword(s):  
Phylogenetic Relationships ◽  
Outer Wall ◽  
Shell Microstructure ◽  
Shell Wall ◽  
Wall Structures ◽  
Phylogenetic Implications ◽  
Cellular Wall ◽  
Superfamily Analysis ◽  
Central Texas

Each of the seven families of rudists (Mollusca, Bivalvia, Hippuritacea) is characterized by distinctive shell-wall architectures which reflect phylogenetic relationships within the superfamily. Analysis of the complex, calcareous, cellular wall of the attached valve of the radiolite rudist Eoradiolites davidsoni (Hill) from the Comanche Cretaceous of Central Texas indicates that its wall architecture is an elaboration of the simpler monopleurid rudist wall and supports possible radiolite-monopleurid relationships.Several well-preserved specimens of E. davidsoni were sectioned, polished, etched, and carbon and gold coated for SEM examination. Maximum shell microstructure detail was displayed by etching with a 0.7% HC1 solution from 80 to 100 seconds.The shell of E. davidsoni comprises a large, thick-walled, conical, attached valve (AV) and a small, very thin, operculate, free valve (FV) (Fig. 1a). The AV shell is two-layered with a thin inner wall, in which original structures are usually obliterated by recrystallization, and a thick, cellular, outer wall.

Phylogenetic relationships ofSalvia(Lamiaceae) in China: Evidence from DNA sequence datasets

2012 ◽  
pp. n/a-n/a
Author(s):  
Qian-Quan Li ◽  
Min-Hui Li ◽  
Qing-Jun Yuan ◽  
Zhan-Hu Cui ◽  
Lu-Qi Huang ◽  
...  
Keyword(s):  
Dna Sequence ◽  
Phylogenetic Relationships

Chromosomal location of genes for resistance to powdery mildew in Chinese wheat lines Jieyan 94-1-1 and Siyan 94-1-2

Hereditas ◽  
2002 ◽  
Vol 136 (3) ◽  
pp. 212-218 ◽  
Author(s):  
XIU QIANG HUANG ◽  
SAI L. K. HSAM ◽  
FRIEDRICH J. ZELLER
Keyword(s):  
Powdery Mildew ◽  
Chromosomal Location ◽  
Wheat Lines ◽  
Chinese Wheat

Detection of the Glanzmann's Thrombasthenia Mutations in Arab and Iraqi-Jewish Patients by Polymerase Chain Reaction and Restriction Analysis of Blood or Urine Samples

1991 ◽  
Vol 66 (04) ◽  
pp. 500-504 ◽  
Author(s):  
H Peretz ◽  
U Seligsohn ◽  
E Zwang ◽  
B S Coller ◽  
P J Newman
Keyword(s):  
Polymerase Chain Reaction ◽  
Base Pair ◽  
Restriction Analysis ◽  
Urine Samples ◽  
Chain Reaction ◽  
Base Pairs ◽  
Glanzmann’S Thrombasthenia ◽  
Glanzmann's Thrombasthenia ◽  
Base Pair Deletion ◽  
Polymerase Chain

SummarySevere Glanzmann's thrombasthenia is relatively frequent in Iraqi-Jews and Arabs residing in Israel. We have recently described the mutations responsible for the disease in Iraqi-Jews – an 11 base pair deletion in exon 12 of the glycoprotein IIIa gene, and in Arabs – a 13 base pair deletion at the AG acceptor splice site of exon 4 on the glycoprotein IIb gene. In this communication we show that the Iraqi-Jewish mutation can be identified directly by polymerase chain reaction and gel electrophoresis. With specially designed oligonucleotide primers encompassing the mutation site, an 80 base pair segment amplified in healthy controls was clearly distinguished from the 69 base pair segment produced in patients. Patients from 11 unrelated Iraqi-Jewish families had the same mutation. The Arab mutation was identified by first amplifying a DNA segment consisting of 312 base pairs in controls and of 299 base pairs in patients, and then digestion by a restriction enzyme Stu-1, which recognizes a site that is absent in the mutant gene. In controls the 312 bp segment was digested into 235 and 77 bp fragments, while in patients there was no change in the size of the amplified 299 bp segment. The mutation was found in patients from 3 out of 5 unrelated Arab families. Both Iraqi-Jewish and Arab mutations were detectable in DNA extracted from blood and urine samples. The described simple methods of identifying the mutations should be useful for detection of the numerous potential carriers among the affected kindreds and for prenatal diagnosis using DNA extracted from chorionic villi samples.

Protein S mRNA in Patients with Protein S Deficiency

1995 ◽  
Vol 73 (05) ◽  
pp. 746-749 ◽  
Author(s):  
E Sacchi ◽  
M Pinotti ◽  
G Marchetti ◽  
G Merati ◽  
L Tagliabue ◽  
...  
Keyword(s):  
Gene Polymorphism ◽  
Total Protein ◽  
Restriction Analysis ◽  
Protein S ◽  
Type I ◽  
Protein S Deficiency ◽  
Phenotypic Analysis ◽  
S Deficiency ◽  
Deficiency Type ◽  
S Genes

SummaryA protein S gene polymorphism, detectable by restriction analysis (BstXI) of amplified exonic sequences (exon 15), was studied in seven Italian families with protein S deficiency. In the 17 individuals heterozygous for the polymorphism the study was extended to platelet mRNA through reverse transcription, amplification and densitometric analysis. mRNA produced by the putative defective protein S genes was absent in three families and reduced to a different extent (as expressed by altered allelic ratios) in four families. The allelic ratios helped to distinguish total protein S deficiency (type I) from free protein S deficiency (type IIa) in families with equivocal phenotypes. This study indicates that the study of platelet mRNA, in association with phenotypic analysis based upon protein S assays in plasma, helps to classify patients with protein S deficiency.

Systematics of Hymenophyllum subgenus Mecodium (Hymenophyllaceae) in Taiwan

2019 ◽  
Vol 44 (4) ◽  
pp. 753-767
Author(s):  
Tian-Chuan Hsu ◽  
Yu-Fang Huang ◽  
Yi-Shan Chao
Keyword(s):  
Phylogenetic Relationships ◽  
The Philippines ◽  
Common Element ◽  
Morphological Investigation ◽  
Eastern Asia ◽  
Type Specimens ◽  
The Status ◽  
Molecular Phylogenetic ◽  
A New Species ◽  
Independent Species

Abstract—Hymenophyllum subg. Mecodium, composed of the taxonomically notorious H. polyanthos and approximately 15 other closely related taxa, is a common element of filmy fern communities in the tropical and subtropical moist forests. In Taiwan, although only H. polyanthos and one or two closely related taxa were recognized in recent studies, considerable morphological variation has been observed among populations throughout the island. Thus, we conducted an extensive morphological investigation, as well as a molecular phylogenetic analysis, to clarify the specific diversity and phylogenetic relationships within Hymenophyllum subg. Mecodium in Taiwan. Field and herbaria surveys helped in recognizing five morphs in Taiwan, mainly differentiated by the combination of certain traits, viz., the presence or absence of stipe wings, general frond size and shape, degree of laminar crispation, sori position, and involucre shape. The different morphs had diverse ecological preferences. The phylogenetic tree, inferred from the sequences of the plastid loci rbcL and rps4-trnS, demonstrated that Hymenophyllum subg. Mecodium materials in Taiwan comprise several well-supported lineages, mostly corresponding to the classification based on morphology. Comparing with the protologues and type specimens of 34 related scientific names, the five morphs are herein recognized as five independent species. A new species, Hymenophyllum exquisitum, is described here. Also, the status of H. paniculiflorum is reconfirmed and that of H. fujisanense, H. parallelocarpum, and H. punctisorum reinstated. Only H. exquisitum and H. parallelocarpum are endemic to Taiwan among all the species studied. In addition, the names Hymenophyllum blumeanum, H. integrum, H. microsorum, H. polyanthos, H. tenellum, and H. wrightii are now excluded from the regional flora, and several related taxa from China, Taiwan, and the Philippines are treated as synonyms. This study unravels the deep phylogenetic relationships within Hymenophyllum subg. Mecodium in Taiwan and Eastern Asia.

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