Hydrolysis of oligosaccharides of the ?-(1?4)-linked d-xylose series by an endo(1?4)-?-d-xylanase from the anaerobic rumen fungus Neocallimastix frontalis

1994 ◽  
Vol 10 (1) ◽  
pp. 64-68 ◽  
Author(s):  
V. Garcia-Campayo ◽  
S. I. McCrae ◽  
T. M. Wood
Keyword(s):  
Rumen Fungus ◽  
Neocallimastix Frontalis ◽  
Hydrolysis Of

Attachment to cellulose and maturation of attached thalli in the anaerobic rumen fungus Neocallimastix frontalis strain RE1

1998 ◽  
Vol 102 (9) ◽  
pp. 1119-1125 ◽  
Author(s):  
Anthony J. Richardson ◽  
Colin S. Stewart ◽  
Graham W. Gooday
Keyword(s):  
Rumen Fungus ◽  
Neocallimastix Frontalis

scholarly journals Chitin Synthase Activity from Neocallimastix frontalis, an Anaerobic Rumen Fungus

Microbiology ◽  
1989 ◽  
Vol 135 (2) ◽  
pp. 279-283 ◽  
Author(s):  
L. GAY ◽  
M. HEBRAUD ◽  
V. GIRARD ◽  
M. FEVRE
Keyword(s):  
Chitin Synthase ◽  
Rumen Fungus ◽  
Neocallimastix Frontalis

scholarly journals Analysis of Functional Genes in Carbohydrate Metabolic Pathway of Anaerobic Rumen Fungus Neocallimastix frontalis PMA02

2009 ◽  
Vol 22 (11) ◽  
pp. 1555-1565 ◽  
Author(s):  
Mi Kwon ◽  
Jaeyong Song ◽  
Jong K. Ha ◽  
Hong-Seog Park ◽  
Jongsoo Chang
Keyword(s):  
Metabolic Pathway ◽  
Functional Genes ◽  
Rumen Fungus ◽  
Neocallimastix Frontalis

scholarly journals Characteristic of a GH10 Xylanase From The Anaerobic Rumen Fungus Anaeromyces Robustus and Application in Bread Making

2021 ◽  
Author(s):  
Zhenyang Liu ◽  
Sitao Wen ◽  
Guogan Wu ◽  
Huawei Wu
Keyword(s):  
Bread Making ◽  
Beechwood Xylan ◽  
Rumen Fungus ◽  
Food Applications ◽  
Rumen Microorganism ◽  
Ph Range ◽  
Tlc Analysis ◽  
Hydrolysis Of ◽  
Gh10 Xylanase

Abstract The rumen of ruminants contains a variety of fungi capable of producing xylanases to break down plant cell walls. In this study, a new GH10 xylanase gene ArXyn10c20 from anaerobic rumen microorganism Anaeromyces robustus was successfully expressed in Pichia Pastoris GS115, with a protein molecular weight of approximately 42 kDa and showed the similarity by 64.08% with the β-Xylanase form Neocallimastix Californiae. The optimal pH and temperature for ArXyn10c20 was 5.5 at 40℃. ArXyn10c20 was stable in the pH range 5.0 – 9.0 for 1h which the residual enzyme activity was all above 75%. The activity of recombinant xylanase was significantly enhanced by 1 mM Cu 2+ . The products of ArXyn10c20 hydrolysis of beechwood xylan were xylobiose, xylotriose and xylotetraose by TLC analysis. In food applications, ArXyn10c20 can significantly improve the quality of dough and bread. With the addition of 7.5 mg ArXyn10c20, the hardness, gumminess and chewiness of the bread decreased by 42.24%, 45.33% and 55.36% respectively and the reducing sugar increased by 18.67%. The new discovered xylanase ArXyn10c20 has great potential in food industry.

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