The relationship between molecular size and electrophoretic mobility in agarose gels as determined from a single population of growing RNA molecules

1975 ◽  
Vol 2 (3) ◽  
pp. 195-201 ◽  
Author(s):  
K. Andersson ◽  
M. Fagerlind ◽  
B. Daneholt
Keyword(s):  
Electrophoretic Mobility ◽  
Molecular Size ◽  
Single Population ◽  
Rna Molecules ◽  
Agarose Gels ◽  
The Relationship

scholarly journals Non-coding RNAs in cancers with chromosomal rearrangements: the signatures, causes, functions and implications

2019 ◽  
Vol 11 (10) ◽  
pp. 886-898 ◽  
Author(s):  
Cai Han ◽  
Lin-Yu Sun ◽  
Wen-Tao Wang ◽  
Yu-Meng Sun ◽  
Yue-Qin Chen
Keyword(s):  
Gene Expression ◽  
Signaling Pathways ◽  
Fusion Proteins ◽  
Gene Fusion ◽  
Chromosomal Rearrangements ◽  
Chromosomal Translocation ◽  
Chromosomal Translocations ◽  
Rna Molecules ◽  
Non Coding Rnas ◽  
The Relationship

Abstract Chromosomal translocation leads to the juxtaposition of two otherwise separate DNA loci, which could result in gene fusion. These rearrangements at the DNA level are catastrophic events and often have causal roles in tumorigenesis. The oncogenic DNA messages are transferred to RNA molecules, which are in most cases translated into cancerous fusion proteins. Gene expression programs and signaling pathways are altered in these cytogenetically abnormal contexts. Notably, non-coding RNAs have attracted increasing attention and are believed to be tightly associated with chromosome-rearranged cancers. These RNAs not only function as modulators in downstream pathways but also directly affect chromosomal translocation or the associated products. This review summarizes recent research advances on the relationship between non-coding RNAs and chromosomal translocations and on diverse functions of non-coding RNAs in cancers with chromosomal rearrangements.

scholarly journals The oncornavirus glycoprotein gp69/71: a constituent of the surface of normal and malignant thymocytes.

1975 ◽  
Vol 141 (1) ◽  
pp. 172-187 ◽  
Author(s):  
B C Del Vellano ◽  
B Nave ◽  
B P Croker ◽  
R A Lerner ◽  
F J Dixon
Keyword(s):  
Leukemia Virus ◽  
Molecular Size ◽  
Size Class ◽  
Antigenic Determinants ◽  
Lymphoma Cells ◽  
Differentiated Cells ◽  
Interesting Difference ◽  
Associated Proteins ◽  
Related Proteins ◽  
The Relationship

The oncornavirus related proteins associated with the surface of normal and malignant thymocytes were studied. Three virion-associated proteins (gp69/71, p45, p30) were associated with lymphoma cells from about 70% of the tumors studied. Two virion-associated proteins (gp69/71 and p45 were associated with normal thymocytes form some but not all strains of mice. In gp69/71- mice, conversion to the gp69/71+ phenotype accompanied leukemogenesis. An interesting difference in the apparent molecular size of virus related antigens of the 70,000 dalton size class was detected in lymphoma cells present in involved spleens as compared to involved thymuses. Mice infected as neonates with Scripps leukemia virus make antibody to gp69/71 and some make antibodies to molecules associated with the surface of their own tumors. The significance of the restricted presence of antigens coded for by the viral genome to the surface of some differentiated cells is discussed in reference to (a) the relationship between virion, leukemia associated, and differentiation dependent markers, and (b) the possible consequence to the host of having similar antigenic determinants on three independent structures with replicative potential (virus, normal thymocytes, and tumor cells).

Variability in the Proportion and Type of Subunits in Lupin Storage Globulins

1975 ◽  
Vol 2 (1) ◽  
pp. 29 ◽  
Author(s):  
JM Gillespie ◽  
RJ Blagrove
Keyword(s):  
Protein Structure ◽  
Electrophoretic Mobility ◽  
Relative Proportion ◽  
Genetic Selection ◽  
Molecular Size ◽  
The Other ◽  
Subunit Composition ◽  
Molecular Weights ◽  
Species Specific ◽  
Conglutin Γ

The seeds of 12 species of lupin were examined and were found to contain two major globulins, conglutins α and β, while some contained a third minor globulin, conglutin �. There were considerable differences between species in the electrophoretic mobility and proportions of conglutins α and β, and in their subunit composition in terms of the number of components, their molecular weights and the importance of disulphide bonding between them. However, the electrophoretic behaviour and subunit composition of conglutins α and β did appear to be species-specific. Conglutin γ, on the other hand, did not seem to vary in molecular size or electrophoretic mobility within this genus. The 18 cultivars of Lupinus angustifolius examined appeared to be more closely related in terms of the number and size of subunits, although variations were apparent in the relative proportion of these subunits, especially with wild types. It is suggested that this variability in the protein structure of lupin globulins may provide evidence that substantial changes can be induced by genetic selection in the composition of these proteins without upsetting their structure-function relations.

The relationship between size and expression of nonmetric traits on the human skull

1969 ◽  
pp. 81-97
Author(s):  
Chelsea Wilson
Keyword(s):  
Genetic Relatedness ◽  
Single Population ◽  
Asian Origin ◽  
Human Skull ◽  
South Eastern ◽  
Skull Size ◽  
Nonmetric Traits ◽  
The Relationship ◽  
Trait Correlations

Nonmetric traits are frequently analyzed in the field of anthropology to measure genetic relatedness, or biodistance, within or between populations. These studies are performed under the assumption that nonmetric traits are genetically inherited. However, much of the research on nonmetric traits has revealed that numerous factors can confound heritability. Skull size is one of the factors that are shown in some samples to have an effect on the expression of nonmetric traits. There is evidence that nonmetric trait expression is population specific; therefore, the current study was performed to determine if size-trait correlations would occur within a single population. Nonmetric traits in a sample of 20 skulls (South Eastern Asian origin) are analyzed to determine if there are correlations between skull size and expression of nonmetric traits. Intertrait correlations are also examined. This type of study is important because if the expression of certain nonmetric traits is related to factors outside of genetics, then those traits would not be useful in biodistance studies. The results of this study indicate that there are no correlations between overall skull size and nonmetric traits. However, correlations were found between individual measurements and nonmetric traits, as well as between traits.

scholarly journals CoDNaS-RNA: a database of Conformational Diversity in the Native State of RNA

2020 ◽  
Author(s):  
Martín González Buitrón ◽  
Ronaldo Romario Tunque Cahui ◽  
Emilio García Ríos ◽  
Layla Hirsh ◽  
María Silvina Fornasari ◽  
...  
Keyword(s):  
Conformational Changes ◽  
Structural Features ◽  
Rna Structures ◽  
Rna Molecules ◽  
Functional Annotations ◽  
Public Resource ◽  
Conformational Diversity ◽  
Dynamics And Function ◽  
And Function ◽  
The Relationship

AbstractConformational changes in RNA native ensembles are central to fulfill many of their biological roles. Systematic knowledge of the extent and possible modulators of this conformational diversity is desirable to better understand the relationship between RNA dynamics and function.We have developed CoDNaS-RNA as the first database of conformational diversity in RNA molecules. Known RNA structures are retrieved and clustered to identify alternative conformers of each molecule. Pairwise structural comparisons within each cluster allows to measure the variability of the molecule. Additional data on structural features, molecular interactions and functional annotations are provided. CoDNaS-RNA is implemented as a public resource that can be of much interest for computational and bench scientists alike.AvailabilityCoDNaS-RNA is freely accessible at http://ufq.unq.edu.ar/[email protected]

Plasmid recombination intermediates generated in a Saccharomyces cerevisiae cell-free recombination system

1985 ◽  
Vol 5 (9) ◽  
pp. 2361-2368
Author(s):  
L S Symington ◽  
P Morrison ◽  
R Kolodner
Keyword(s):  
Electron Microscopy ◽  
Saccharomyces Cerevisiae ◽  
Electrophoretic Mobility ◽  
Electrophoretic Analysis ◽  
Individual Band ◽  
Agarose Gels ◽  
Cell Extracts ◽  
Recombination System ◽  
Plasmid Recombination

We have developed an assay utilizing Saccharomyces cerevisiae cell extracts to catalyze recombination in vitro between homologous plasmids containing different mutant alleles of the tet gene. Electrophoretic analysis of product DNA indicated that a number of novel DNA species were formed during the reaction. These species migrated through agarose gels as distinct bands with decreased electrophoretic mobility compared with the substrate DNA. The DNA from each individual band was purified and shown to be enriched 5- to 100-fold for tetracycline-resistant recombinants by using a transformation assay. The structure of the DNA molecules present in these bands was determined by electron microscopy. Recombination between circular substrates appeared to involve the formation and processing of figure-eight molecules, while recombination between circular and linear substrates involved the formation of molecules in which a circular monomer had a monomer-length linear tail attached at a region of homology.

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