RNA polymerase II transcription and the functional organization of the mammalian cell nucleus

Chromosoma ◽  
1995 ◽  
Vol 103 (8) ◽  
pp. 509-516 ◽  
Author(s):  
Michael J. Hendzel ◽  
David P. Bazett-Jones
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Mammalian Cell ◽  
Cell Nucleus ◽  
Functional Organization ◽  
Rna Polymerase Ii Transcription

scholarly journals Spatial Organization of RNA Polymerase II Revealed by Super-Resolution Imaging of Mammalian Cell Nucleus

2013 ◽  
Vol 104 (2) ◽  
pp. 339a
Author(s):  
Ziqing Zhao ◽  
Rahul Roy ◽  
J. Christof M. Gebhardt ◽  
David M. Suter ◽  
Alec R. Chapman ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Mammalian Cell ◽  
Cell Nucleus ◽  
Spatial Organization ◽  
Super Resolution ◽  
Resolution Imaging

Organization of transcription and pre-mRNA splicing within the mammalian cell nucleus

1995 ◽  
Vol 53 ◽  
pp. 768-769
Author(s):  
D.L. Spector ◽  
S. Huang ◽  
S. Kaurin
Keyword(s):  
Rna Polymerase Ii ◽  
Cell Nucleus ◽  
Functional Organization ◽  
Mrna Splicing ◽  
Splicing Factors ◽  
Interchromatin Granule Clusters ◽  
Interchromatin Granule ◽  
Nuclear Regions ◽  
Relationship Of ◽  
Perichromatin Fibrils

We have been interested in the organization of RNA polymerase II transcription and pre-mRNA splicing within the cell nucleus. Several models have been proposed for the functional organization of RNA within the eukaryotic nucleus and for the relationship of this organization to the distribution of pre-mRNA splicing factors. One model suggests that RNAs which must be spliced are capable of recruiting splicing factors to the sites of transcription from storage and/or reassembly sites. When one examines the organization of splicing factors in the nucleus in comparison to the sites of chromatin it is clear that splicing factors are not localized in coincidence with heterochromatin (Fig. 1). Instead, they are distributed in a speckled pattern which is composed of both perichromatin fibrils and interchromatin granule clusters. The perichromatin fibrils are distributed on the periphery of heterochromatin and on the periphery of interchromatin granule clusters as well as being diffusely distributed throughout the nucleoplasm. These nuclear regions have been previously shown to represent initial sites of incorporation of 3H-uridine.

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