Characterization of a cDNA encoding a PSII-associated chlorophyll a/b-binding protein (CAB) from Chlamydomonas moewusii fitting into neither type I nor type II

1991 ◽  
Vol 19 (4) ◽  
pp. 285-288 ◽  
Author(s):  
Lucie Larouche ◽  
Colette Tremblay ◽  
Claire Simard ◽  
Guy Bellemare
Keyword(s):  
Chlorophyll A ◽  
Binding Protein ◽  
Type I ◽  
Type Ii ◽  
Chlamydomonas Moewusii

scholarly journals Characterization of a Type II Chlorophyll a/b-binding Protein Gene (Lhcb2*Pp1) in Peach: I. Isolation, Identification, and Abundance in Developing Leaves of Mature `Loring' Trees in the Absence of Flowering

1998 ◽  
Vol 123 (4) ◽  
pp. 486-492 ◽  
Author(s):  
Carole Bassett ◽  
Ann Callahan ◽  
Linda Dunn
Keyword(s):  
Prunus Persica ◽  
Protein Gene ◽  
Binding Protein ◽  
Amino Acid Level ◽  
Growing Season ◽  
Type I ◽  
Type Ii ◽  
Genes Encoding ◽  
Binding Protein Gene

A gene (Lhcb2*Pp1) encoding a type II chlorophyll a/b-binding protein (LHCB) was isolated from peach [Prunus persica (L.) Batsch]. The gene was sequenced and compared to a variety of other genes encoding LHCB polypeptides associated with photosystem II. Similarity at the nucleotide and amino acid level was highest between Lhcb2*Pp1 and other type II genes and was lowest with type I and III genes from other species. Expression of Lhcb2*Pp1 was followed by determining abundance of transcripts in developing leaves of field grown trees in the absence of flowering. Expression was monitored at three times during the first phase of the growing season and was shown to be highest in leaves which were at or near full expansion at each sampling time. These results are consistent with the function of this gene and indicate that it can serve as a marker of photosynthetic maturity under field conditions.

Nonidentical Induction of the Guanylate Binding Protein and the 56K Protein by Type I and Type II Interferons

1986 ◽  
Vol 6 (4) ◽  
pp. 417-427 ◽  
Author(s):  
YIH-SHYUN E. CHENG ◽  
MARY F. BECKER-MANLEY ◽  
THAI D. NGUYEN ◽  
WILLIAM F. DEGRADO ◽  
GERALD J. JONAK
Keyword(s):  
Binding Protein ◽  
Type I ◽  
Type Ii ◽  
Guanylate Binding Protein

scholarly journals Comprehensive Characterization of Toxoplasma Acyl Coenzyme A-Binding Protein TgACBP2 and Its Critical Role in Parasite Cardiolipin Metabolism

mBio ◽  
2018 ◽  
Vol 9 (5) ◽  
Author(s):  
Yong Fu ◽  
Xia Cui ◽  
Sai Fan ◽  
Jing Liu ◽  
Xiao Zhang ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Lipid Metabolism ◽  
Toxoplasma Gondii ◽  
Binding Protein ◽  
Ankyrin Repeat ◽  
Type I ◽  
Type Ii ◽  
Content Type ◽  
High K ◽  
Acyl Coenzyme A

ABSTRACT Acyl coenzyme A (CoA)-binding protein (ACBP) can bind acyl-CoAs with high specificity and affinity, thus playing multiple roles in cellular functions. Mitochondria of the apicomplexan parasite Toxoplasma gondii have emerged as key organelles for lipid metabolism and signaling transduction. However, the rationale for how this parasite utilizes acyl-CoA-binding protein to regulate mitochondrial lipid metabolism remains unclear. Here, we show that an ankyrin repeat-containing protein, TgACBP2, is localized to mitochondria and displays active acyl-CoA-binding activities. Dephosphorylation of TgACBP2 is associated with relocation from the plasma membrane to the mitochondria under conditions of regulation of environmental [K+]. Under high [K+] conditions, loss of ACBP2 induced mitochondrial dysfunction and apoptosis-like cell death. Disruption of ACBP2 caused growth and virulence defects in the type II strain but not in type I parasites. Interestingly, mitochondrial association factor-1 (MAF1)-mediated host mitochondrial association (HMA) restored the growth ability of ACBP2-deficient type II parasites. Lipidomics analysis indicated that ACBP2 plays key roles in the cardiolipin metabolism of type II parasites and that MAF1 expression complemented the lipid metabolism defects of ACBP2-deficient type II parasites. In addition, disruption of ACBP2 caused attenuated virulence of Prugniuad (Pru) parasites for mice. Taking the results collectively, these data indicate that ACBP2 is critical for the growth and virulence of type II parasites and for the growth of type I parasites under high [K+] conditions. IMPORTANCE Toxoplasma gondii is one of the most successful human parasites, infecting nearly one-third of the total world population. T. gondii tachyzoites residing within parasitophorous vacuoles (PVs) can acquire fatty acids both via salvage from host cells and via de novo synthesis pathways for membrane biogenesis. However, although fatty acid fluxes are known to exist in this parasite, how fatty acids flow through Toxoplasma lipid metabolic organelles, especially mitochondria, remains unknown. In this study, we demonstrated that Toxoplasma expresses an active ankyrin repeat containing protein TgACBP2 to coordinate cardiolipin metabolism. Specifically, HMA acquisition resulting from heterologous functional expression of MAF1 rescued growth and lipid metabolism defects in ACBP2-deficient type II parasites, manifesting the complementary role of host mitochondria in parasite cardiolipin metabolism. This work highlights the importance of TgACBP2 in parasite cardiolipin metabolism and provides evidence for metabolic association of host mitochondria with T. gondii.

Evaluation of an enzyme immunoassay kit for estrogen receptor measurement--report II.

1988 ◽  
Vol 34 (10) ◽  
pp. 2053-2057 ◽  
Author(s):  
S Raam ◽  
D M Vrabel
Keyword(s):  
Enzyme Immunoassay ◽  
Binding Sites ◽  
Solid Phase ◽  
Functional Characterization ◽  
Quantitative Agreement ◽  
Type I ◽  
Type Ii ◽  
Immunoreactive Protein ◽  
Estrogen Binding

Abstract We present evidence to show that monoclonal antibodies to estrogen receptors (ER) in solid phase recognize the secondary estrogen binding sites with moderate to low affinity for estradiol (E2). An excellent quantitative agreement was found in five cytosols between the ER values obtained by the enzyme immunoassay (ER-EIA) and the amount of secondary estrogen binding sites measured by the assay involving dextran-coated charcoal (Clin Chem 1986;32:1496). The immunoreactive protein recognized by the antibody-coated beads, when allowed to react with ER(+) cytosols, is shown to bind [3H]estradiol only when the ligand concentration exceeds 8 nmol/L. Further biochemical and functional characterization of the immunoreactive protein is required to establish similarities/dissimilarities between this protein, high-affinity Type I ER sites, and the secondary sites such as Type II sites.

Geochemical characterization of hydrocarbon source rocks of the Triassic-Jurassic time interval in the Mandawa basin, southern Tanzania: Implications for petroleum generation potential

2020 ◽  
Vol 123 (4) ◽  
pp. 587-596
Author(s):  
A. Emanuel ◽  
C.H. Kasanzu ◽  
M. Kagya
Keyword(s):  
Source Rocks ◽  
Geochemical Data ◽  
Time Interval ◽  
Type I ◽  
Hydrocarbon Generation ◽  
Type Ii ◽  
Geochemical Characterization ◽  
Type Iii ◽  
Petroleum Generation

Abstract Triassic to mid-Jurassic core samples of the Mandawa basin, southern Tanzania (western coast of the Indian Ocean), were geochemically analyzed in order to constrain source rock potentials and petroleum generation prospects of different stratigraphic formations within the coastal basin complex. The samples were collected from the Mihambia, Mbuo and Nondwa Formations in the basin. Geochemical characterization of source rocks intersected in exploration wells drilled between 503 to 4042 m below surface yielded highly variable organic matter contents (TOC) rated between fair and very good potential source rocks (0.5 to 8.7 wt%; mean ca. 2.3 wt%). Based on bulk geochemical data obtained in this study, the Mandawa source rocks are mainly Type I, Type II, Type III, mixed Types II/III and Type IV kerogens, with a predominance of Type II, Type III and mixed Type II/III. Based on pyrolysis data (Tmax 417 to 473oC; PI = 0.02 to 0.47; highly variable HI = 13 to 1 000 mg/gTOC; OI = 16 to 225 mg/g; and VR values of between 0.24 to 0.95% Ro) we suggest that the Triassic Mbuo Formation and possibly the mid-Jurassic Mihambia Formation have a higher potential for hydrocarbon generation than the Nondwa Formation as they are relatively thermally mature.

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