Effects of somatostatin-25 on lipid mobilization from rainbow trout, Oncorhynchus mykiss, liver and adipose tissue incubated in vitro. Comparison with somatostatin-14

1994 ◽  
Vol 164 (3) ◽  
pp. 256-260 ◽  
Author(s):  
C. D. Eilertson ◽  
M. A. Sheridan
Keyword(s):  
Adipose Tissue ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Lipid Mobilization ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
In Vitro Comparison ◽  
Somatostatin 14

scholarly journals Genistein Induces Adipogenic and Autophagic Effects in Rainbow Trout (Oncorhynchus mykiss) Adipose Tissue: In Vitro and In Vivo Models

2020 ◽  
Vol 21 (16) ◽  
pp. 5884
Author(s):  
Sara Balbuena-Pecino ◽  
Esmail Lutfi ◽  
Natàlia Riera-Heredia ◽  
Esther Gasch-Navalón ◽  
Emilio J. Vélez ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Rainbow Trout ◽  
Lipid Metabolism ◽  
Oncorhynchus Mykiss ◽  
Fatty Acid Synthase ◽  
Hormone Sensitive Lipase ◽  
In Vivo Models ◽  
Rainbow Trout Oncorhynchus Mykiss

Soybeans are one of the most used alternative dietary ingredients in aquafeeds. However, they contain phytoestrogens like genistein (GE), which can have an impact on fish metabolism and health. This study aimed to investigate the in vitro and in vivo effects of GE on lipid metabolism, apoptosis, and autophagy in rainbow trout (Oncorhynchus mykiss). Primary cultured preadipocytes were incubated with GE at different concentrations, 10 or 100 μM, and 1 μM 17β-estradiol (E2). Furthermore, juveniles received an intraperitoneal injection of GE at 5 or 50 µg/g body weight, or E2 at 5 µg/g. In vitro, GE 100 μM increased lipid accumulation and reduced cell viability, apparently involving an autophagic process, indicated by the higher LC3-II protein levels, and higher lc3b and cathepsin d transcript levels achieved after GE 10 μM. In vivo, GE 50 µg/g upregulated the gene expression of fatty acid synthase (fas) and glyceraldehyde-3-phosphate dehydrogenase in adipose tissue, suggesting enhanced lipogenesis, whereas it increased hormone-sensitive lipase in liver, indicating a lipolytic response. Besides, autophagy-related genes increased in the tissues analyzed mainly after GE 50 µg/g treatment. Overall, these findings suggest that an elevated GE administration could lead to impaired adipocyte viability and lipid metabolism dysregulation in rainbow trout.

Estrogenic activity of multicyclic aromatic hydrocarbons in rainbow trout (Oncorhynchus mykiss) in vitro assays

2019 ◽  
Vol 207 ◽  
pp. 43-51 ◽  
Author(s):  
Richard C. Kolanczyk ◽  
Jeffrey S. Denny ◽  
Barbara R. Sheedy ◽  
Patricia K. Schmieder ◽  
Mark A. Tapper
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Aromatic Hydrocarbons ◽  
Estrogenic Activity ◽  
In Vitro Assays ◽  
Rainbow Trout Oncorhynchus Mykiss

scholarly journals Gill amoebae from freshwater rainbow trout ( Oncorhynchus mykiss ): In vitro evaluation of antiparasitic compounds against Vannella sp.

2020 ◽  
Vol 43 (6) ◽  
pp. 665-672
Author(s):  
Hannah Malene Jensen ◽  
Asma Mohammad Karami ◽  
Heidi Mathiessen ◽  
Azmi Al‐Jubury ◽  
Per Walter Kania ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
In Vitro Evaluation ◽  
Rainbow Trout Oncorhynchus Mykiss
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