Comparative analysis of the region of the mitochondrial genome contaning the ATPase subunit 9 gene in the two related yeast species Saccharomyces douglasii and Saccharomyces cerevisiae

1994 ◽  
Vol 25 (6) ◽  
pp. 504-507 ◽  
Author(s):  
L. Nicoletti ◽  
P. Laveder ◽  
R. Pellizzari ◽  
B. Cardazzo ◽  
G. Carignani
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Comparative Analysis ◽  
Mitochondrial Genome ◽  
Yeast Species ◽  
Atpase Subunit ◽  
Subunit 9

Inhibition of Yeast Growth by Tryptamine and Recovery with Tryptophan

2020 ◽  
Vol 16 (1) ◽  
pp. 48-52 ◽  
Author(s):  
Chandrika Kadkol ◽  
Ian Macreadie
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Carbon Source ◽  
Competitive Inhibition ◽  
Yeast Species ◽  
Inhibitory Effect ◽  
The Body ◽  
Inhibitory Effects ◽  
Trna Synthetases ◽  
Fermentative Growth ◽  
Biogenic Monoamine

Background: Tryptamine, a biogenic monoamine that is present in trace levels in the mammalian central nervous system, has probable roles as a neurotransmitter and/or a neuromodulator and may be associated with various neuropsychiatric disorders. One of the ways tryptamine may affect the body is by the competitive inhibition of the attachment of tryptophan to tryptophanyl tRNA synthetases. Methods: This study has explored the effects of tryptamine on growth of six yeast species (Saccharomyces cerevisiae, Candida glabrata, C. krusei, C. dubliniensis, C. tropicalis and C. lusitaniae) in media with glucose or ethanol as the carbon source, as well as recovery of growth inhibition by the addition of tryptophan. Results: Tryptamine was found to have an inhibitory effect on respiratory growth of all yeast species when grown with ethanol as the carbon source. Tryptamine also inhibited fermentative growth of Saccharomyces cerevisiae, C. krusei and C. tropicalis with glucose as the carbon source. In most cases the inhibitory effects were reduced by added tryptophan. Conclusion: The results obtained in this study are consistent with tryptamine competing with tryptophan to bind mitochondrial and cytoplasmic tryptophanyl tRNA synthetases in yeast: effects on mitochondrial and cytoplasmic protein synthesis can be studied as a function of growth with glucose or ethanol as a carbon source. Of the yeast species tested, there is variation in the sensitivity to tryptamine and the rescue by tryptophan. The current study suggests appropriate yeast strains and approaches for further studies.

scholarly journals Mitochondrial Genome Evolution in a Single Protoploid Yeast Species

2012 ◽  
Vol 2 (9) ◽  
pp. 1103-1111 ◽  
Author(s):  
Paul P. Jung ◽  
Anne Friedrich ◽  
Cyrielle Reisser ◽  
Jing Hou ◽  
Joseph Schacherer
Keyword(s):  
Mitochondrial Genome ◽  
Genome Evolution ◽  
Yeast Species ◽  
Mitochondrial Genome Evolution

scholarly journals Nucleotide sequence and transcription of the sugar beet mitochondrial F0F1-ATPase subunit 9 gene

1989 ◽  
Vol 17 (21) ◽  
pp. 8857-8857 ◽  
Author(s):  
Yongbiae Xue ◽  
Colwyn M. Thomas ◽  
D.Roy Davies
Keyword(s):  
Nucleotide Sequence ◽  
Sugar Beet ◽  
Atpase Subunit ◽  
Subunit 9

Conversion at large intergenic regions of mitochondrial DNA in Saccharomyces cerevisiae

1990 ◽  
Vol 10 (4) ◽  
pp. 1530-1537
Author(s):  
P J Skelly ◽  
G D Clark-Walker
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Mitochondrial Dna ◽  
Rrna Gene ◽  
Supporting Evidence ◽  
Unexpected Result ◽  
Wild Type ◽  
Mitochondrial Dna Deletion ◽  
Direct Role ◽  
Atpase Subunit ◽  
Intergenic Regions

Saccharomyces cerevisiae mitochondrial DNA deletion mutants have been used to examine whether base-biased intergenic regions of the genome influence mitochondrial biogenesis. One strain (delta 5.0) lacks a 5-kilobase (kb) segment extending from the proline tRNA gene to the small rRNA gene that includes ori1, while a second strain (delta 3.7) is missing a 3.7-kb region between the genes for ATPase subunit 6 and glutamic acid tRNA that encompasses ori7 plus ori2. Growth of these strains on both fermentable and nonfermentable substrates does not differ from growth of the wild-type strain, indicating that the deletable regions of the genome do not play a direct role in the expression of mitochondrial genes. Examination of whether the 5- or 3.7-kb regions influence mitochondrial DNA transmission was undertaken by crossing strains and examining mitochondrial genotypes in zygotic colonies. In a cross between strain delta 5.0, harboring three active ori elements (ori2, ori3, and ori5), and strain delta 3.7, containing only two active ori elements (ori3 and ori5), there is a preferential recovery of the genome containing two active ori elements (37% of progeny) over that containing three active elements (20%). This unexpected result, suggesting that active ori elements do not influence transmission of respiratory-competent genomes, is interpreted to reflect a preferential conversion of the delta 5.0 genome to the wild type (41% of progeny). Supporting evidence for conversion over biased transmission is shown by preferential recovery of a nonparental genome in the progeny of a heterozygous cross in which both parental molecules can be identified by size polymorphisms.

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