c-Fos protooncogene expression in human normal peripheral blood leukocytes

1987 ◽  
Vol 1 (1) ◽  
pp. 61-64
Author(s):  
Francesco Colotta ◽  
Nadia Polentarutti ◽  
Ji Ming Wang ◽  
Alberto Mantovani
Keyword(s):  
Peripheral Blood ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Normal Peripheral Blood

The effects of doxorubicin on apoptosis and adhesion molecules of normal peripheral blood leukocytes—an ex vivo study

2003 ◽  
Vol 14 (5) ◽  
pp. 383-389 ◽  
Author(s):  
Pnina Ciobotaro ◽  
Liat Drucker ◽  
Avivit Neumann ◽  
Hava Shapiro ◽  
Jermey Shapira ◽  
...  
Keyword(s):  
Adhesion Molecules ◽  
Peripheral Blood ◽  
Ex Vivo ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Normal Peripheral Blood ◽  
Ex Vivo Study

A quantitative immunoscanning EM analysis of CD16 (FeRIII) antigen on peripheral blood leukocytes

1990 ◽  
Vol 48 (3) ◽  
pp. 866-867
Author(s):  
Eduardo Fernández-Segura ◽  
O. Manuel Garcia-Lopez ◽  
Antonio Gutierrez ◽  
Antonio Campos
Keyword(s):  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Cell Types ◽  
Immunogold Labelling ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Normal Peripheral Blood ◽  
Histocompatibility Complex ◽  
Backscattered Electron ◽  
Electron Imaging

A common feature of many cell types which mediate major histocompatibility complex (MCH)-unrestricted lysis is the presence of receptors for the Fc region of IgG (FcR) on the surface. Monoclonal antibodies (MAbs) against these receptors have identified three distinct FcR, i.e., FcRI, FcRII, FcRIII. These receptors are expressed on different but overlapping cells populations, the level of FcR expression being a characteristc of each populations. FcRIII (CD16 antigen) is the low-affinity IgG Fc-receptor whLch is predominantly expressed on human natural killer (NK) cells and granulocytes. With the recent application of immunogold labelling for scanning electron microscopy (immuno-SEM) in the backscattered electron imaging (BEI) mode allow us to correlate antigenic expression with cell surface morphology. In this report we analize the expression and distribution of the CD16 (FcRIII) antigen on peripheral blood leukocytes as observed with immuno-SEM.Material and Methods. Mononuclear cells (MC) were isolated from normal peripheral blood using Ficoll-Paque (Pharmacia Fine Chemicals, Uppsala, Sweden).

High-Throughput Sequencing for Diagnosis, Prognosis and Monitoring of Lymphoid Malignancies

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 3779-3779
Author(s):  
Scott D Boyd ◽  
Jason D. Merker ◽  
James L. Zehnder ◽  
Andrew Z Fire
Keyword(s):  
High Throughput ◽  
Peripheral Blood ◽  
Read Length ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Normal Peripheral Blood ◽  
Dilution Series ◽  
Lymphoid Malignancies ◽  
Immune Receptors

Abstract New high-throughput DNA sequencing methods have many advantages for the analysis of rearranged immunoglobulin (Ig) and T cell receptor (TCR) loci in suspected lymphoid malignancies. Instead of primarily analyzing the size distribution of rearranged immune receptors using gel or capillary electrophoresis, sequencing complex nucleic acid pools provides much more complete characterization of the receptor sequences present. Currently, separate assays are used to evaluate Ig and TCR clonality, Ig hypermutation status, and to test for residual malignant cells after treatment. We used multiplexed PCR amplification of immunoglobulin heavy chain loci for high-throughput pyrosequencing with the Roche 454 platform to evaluate the detection of clonal IgH sequences, determination of hypermutation status, and sensitivity of detection of low levels of a known clonal receptor. Our method also evaluated the feasibility of physical “bar-coding” of DNA molecules derived from different samples to enable cost-effective sequencing of sample pools. 23 pooled specimens were sequenced including: known lymphoma specimens (4); peripheral blood leukocytes from normal individuals (4); specimens with indeterminate or discordant results by conventional clonality assays (7); and a dilution series of a clonal CLL/SLL specimen into normal peripheral blood leukocytes. Our initial experiment characterized 522,099 IgH sequences from these specimens, with a mean read length of 242 base pairs. We have employed IgBlast (NCBI) for initial alignment of rearranged IgH sequences to germline V, D and J gene segments. Our data demonstrate that clonal IgH populations can be readily detected and characterized as to their germline or hypermutated status and unique V-D and D-J junctions with this approach. In our dilution series experiment, the clonal receptor sequence could be reliably observed after 1000-fold dilution. Normal patient specimens show expected extensive sequence diversity, and we are exploring methods for optimally summarizing and representing these data. Some PCR artifacts, as well as non-immunoglobulin sequences commonly amplified by current IgH primer sets were also detected, and suggest avenues for further improvement of the methods used for detection and characterization of clonal immune receptors. More broadly, these methods also offer a novel approach to monitoring normal immune responses such as the response of patients to vaccination, and of characterizing the immune system in patients with autoimmune diseases.

FUNCTIONING OF NO-SYNTHASE IN THE PERIPHERAL BLOOD LEUKOCYTES IN PATIENTS WITH ACNE VULGARIS

2018 ◽  
Vol 14 (66) ◽  
pp. 075
Author(s):  
H. S. Lavryk ◽  
O. P. Korniychuk ◽  
Z. Ya. Fedorovych ◽  
Z. D. Vorobets
Keyword(s):  
Peripheral Blood ◽  
Acne Vulgaris ◽  
No Synthase ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes
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