Evolution of growth responses to spleen protein in cultures of pig kidney cells

1965 ◽  
Vol 68 (4) ◽  
pp. 494-501 ◽  
Author(s):  
Kenzo Takata ◽  
Morgan Harris
Keyword(s):  
Kidney Cells ◽  
Growth Responses ◽  
Pig Kidney ◽  
Pig Kidney Cells

scholarly journals NUCLEOLAR AND NUCLEAR RNA SYNTHESIS DURING THE CELL LIFE CYCLE IN MONKEY AND PIG KIDNEY CELLS IN VITRO

1967 ◽  
Vol 33 (2) ◽  
pp. 273-279 ◽  
Author(s):  
Jane L. Showacre ◽  
W. G. Cooper ◽  
D. M. Prescott
Keyword(s):  
Life Cycle ◽  
Rna Synthesis ◽  
Kidney Cells ◽  
Pig Kidney ◽  
Rna Labeling ◽  
Cell Life ◽  
Nuclear Rna ◽  
Nucleolar Rna ◽  
Pig Kidney Cells

The incorporation of 5-3H-uridine and 5-3H-cytidine into nucleolar and nonnucleolar RNA in the nucleus of monkey and pig kidney cells was measured in vitro during the cell life cycle. Time-lapse cinematographic records were made of cells during asynchronous exponential proliferation, in order to identify the temporal position of individual cells in relation to the preceding mitosis. Immediately following cinematography, cells were labeled with uridine-3H and cytidine-3H for a short period, fixed, and analyzed by radioautography. Since the data permit correlation of the rate of RNA labeling with the position of a cell within the cycle, curves could be constructed describing the rate of RNA synthesis over the average cell cycle. RNA synthesis was absent in early telophase, and rose very abruptly in rate in late telophase and in very early G1 in both the nucleus and the reconstituting nucleolus. Thereafter, through the G1 and S periods the rate of nuclear RNA synthesis rose gradually. When we used a 10-min pulse, there was no detectable change in the rate for nucleolar RNA labeling in monkey kidney cells during G1 or S. When we used a 30-min labeling time, the rate of nucleolar RNA labeling rose gradually in pig kidney cells. With increasing time after mitosis, the data became more variable, which may, in part, be related to the variation in generation times for individual cells.

The Induction of Chromosomal Aberrations in Pig Kidney Cells by Far-Red Light

1971 ◽  
Vol 45 (2) ◽  
pp. 274 ◽  
Author(s):  
S. A. Gordon ◽  
A. N. Stroud ◽  
C. H. Chen
Keyword(s):  
Chromosomal Aberrations ◽  
Red Light ◽  
Kidney Cells ◽  
Pig Kidney ◽  
Pig Kidney Cells
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