More accurate chronological age determination of crustaceans from field situations using the physiological age marker, lipofuscin

1994 ◽  
Vol 121 (2) ◽  
pp. 237-245 ◽  
Author(s):  
M. R. J. Sheehy ◽  
J. G. Greenwood ◽  
D. R. Fielder
Keyword(s):  
Age Determination ◽  
Physiological Age ◽  
Chronological Age

Age-dependance of pteridines in the malaria vector, Anopheles stephensi

Pteridines ◽  
2017 ◽  
Vol 28 (3-4) ◽  
pp. 157-161 ◽  
Author(s):  
Hamideh Edalat ◽  
Mohammad Akhoundi ◽  
Hamidreza Basseri
Keyword(s):  
Anopheles Stephensi ◽  
Age Determination ◽  
Ring Structure ◽  
Physiological Age ◽  
Whole Body ◽  
Malaria Vectors ◽  
Significant Difference ◽  
Age Grading ◽  
Linear Manner

AbstractDetermining the accurate age of malaria vectors is crucial to measure the risk of malaria transmission. A group of fluorescent chemicals derived from a pyrimidine-pyrazine ring structure known as pteridines from the head, thorax and whole body of adult female Anopheles stephensi were identified and evaluated as a tool for chronological and physiological age determination of malaria vectors. The female mosquitoes were collected from an insectary colony at an interval of every 5 days, up to 30 days, and the pteridines of head, thorax and whole body were detected fluorometrically by high-pressure liquid chromatography (HPLC) using excitation and emission wavelengths of 365 and 455 nm, respectively. In addition, alteration of the pteridines compounds was compared between blood and sugar fed mosquito groups. Although four pteridines including pterin-6-carboxylic acid, biopterin, xanthopterin and isoxanthopterin were detected, some of them were absent in the head or thorax of mosquitoes. Levels of all four pteridines were similarly decreased in a linear manner throughout 30 days. No significant difference in alteration of pteridine compounds was observed between the two groups of blood or sugar fed mosquitoes. This result indicates that diet has a little effect on pteridines alteration. Age determination based on pteridines, as an age-grading technique, could be used for field collected mosquitoes, which have either sugar or blood meal. In addition, analyzing total pteridine fluorescence from only whole body could be a convenient method to estimate the age.

Age-grading in blackflies (Diptera: Simuliidae) by ovariolar morphology

1995 ◽  
Vol 85 (3) ◽  
pp. 339-344 ◽  
Author(s):  
A.I. Gryaznov
Keyword(s):  
Age Determination ◽  
Neutral Red ◽  
Physiological Age ◽  
Gonotrophic Cycle ◽  
Egg Development ◽  
Age Grading

AbstractA method for physiological age determination in blackflies is described from studies on Simulium equinum (Linnaeus), S. ornatum Meigen, S. erythrocephalum (De Geer), and S. nigrum (Meigen) in Russia, and S. vittatum (Zetterstedt), and S.verecundum-venustum supercomplex in Canada. The method uses neutral red to stain the ovariolar structures which indicate preceding gonotrophic cycles. Two different processes can occur in the ovariole during the gonotrophic cycle: egg development with ovulation, or degeneration of a terminal follicle. Egg-sacs are resorbed and form zones of granulation which accumulate in the ovariole at each gonotrophic cycle. Following degeneration of the terminal follicle, a follicular relic (═gonotrophic dilatation) forms. The sum of zones of granulation plus follicular relics in each ovariole can indicate the number of completed gonotrophic cycles. However the number of follicular relics alone does not correspond to the number of preceding gonotrophic cycles and cannot reliably be used for determination of the parity of a female. Female flies collected in nature were categorized as: nulliparous; 1-parous; or multiparous. In multiparous females, most ovarioles have no more than two zones of granulation or a combination consisting of one zone of granulation and a follicular relic. Females in this category are considered to be 2-parous. In some ovarioles of parous females the germarium and terminal follicles were found to have disappeared.

Analysis of Fibronectin and TGF-β1 Immunoexpression to Determination of Wound Vitality in Wistar Rats (Rattus norvegicus)

2015 ◽  
Vol 1 (1) ◽  
Author(s):  
Nita Novita ◽  
Hasrayati Agustina ◽  
Bethy S. Hernowo ◽  
Abdul H. Hassan
Keyword(s):  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Age Determination ◽  
Scientific Field ◽  
Wound Age ◽  
Forensic Practice ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Tgf Β1

Wound examination is indispensable in forensic practice. The scientific field of wound age determination has advanced progressively during recent years.The purpose of this study was to determine the differences of fibronectin and TGF-β1 expression in both antemortem and postmortem wounds. This study was an experimental with completely randomized design.  The skin wounds (vital and postmortem) were taken from fourty Wistar rats and divided into 10 groups of rats. Immunohistochemical staining was performed to determine the differences between antemortem and postmortem wounds. The result showed that in 30 minutes after antemortem wound infliction, all of samples showed weak reactivity for fibronectin and TGF-β1 (100%).  In first hour after wound infliction, 3 samples (75%) showed weakly positive and 1 sample (25%) strongly positive for fibronectin and TGF-β1.  In 2 hour after wound infliction, 1 sample (25%) showed weakly positive and 3 sample (75%) strongly positive for fibronectin and TGF-β1.  In 3 and 4 hour after wound infliction, all of samples strongly positive for fibronectin and TGF-β1.  In postmortem wound, all of samples showed negativity for fibronectin and TGF-β1. In conclusion, fibronectin and TGF-β1 may be useful in the determination of wound vitality. Keywords: wound, fibronectin, TGF-β1, vitality

scholarly journals Directly ageing the Caribbean spiny lobster, Panulirus argus with validated band counts from gastric mill ossicles

2018 ◽  
Vol 76 (2) ◽  
pp. 442-451 ◽  
Author(s):  
Gaya Gnanalingam ◽  
Mark J Butler ◽  
Thomas R Matthews ◽  
Emily Hutchinson ◽  
Raouf Kilada
Keyword(s):  
Spiny Lobster ◽  
Age Determination ◽  
Florida Keys ◽  
Panulirus Argus ◽  
Gastric Mill ◽  
Coefficients Of Variation ◽  
The Caribbean ◽  
First Time ◽  
Reader Experience

Abstract In crustaceans, ecdysis was long believed to result in the loss and replacement of all calcified structures, precluding the use of conventional ageing methods. However, the discovery of bands in the gastric ossicles of several crustaceans with some correlation with age suggests that direct age estimation may be possible. We applied this method to a tropical spiny lobster, Panulirus argus, one of the most iconic and economically valuable species in the Caribbean. The presence of growth bands was investigated using wild lobsters of unknown age and was validated with captive reared lobsters of known age (1.5–10 years) from the Florida Keys, Florida (USA). Bands were consistently identified in ptero- and zygo-cardiac ossicles of the gastric mill and did not appear to be associated with moulting. Validation with known age animals confirms that bands form annually. Counts between independent readers were reproducible with coefficients of variation ranging from 11% to 26% depending on reader experience and the structure used. This study demonstrates, for the first time, that direct age determination of P. argus is possible.

Using the isotope dating of endocontact hybrid rocks for the age determination of mafic rocks (southern Siberian craton)

2013 ◽  
Vol 54 (11) ◽  
pp. 1340-1351 ◽  
Author(s):  
D.P. Gladkochub ◽  
T.V. Donskaya ◽  
M.T.D. Wingate ◽  
A.M. Mazukabzov ◽  
S.A. Pisarevsky ◽  
...  
Keyword(s):  
Siberian Craton ◽  
Age Determination ◽  
Mafic Rocks ◽  
Isotope Dating

Age determination of the African lion (Panthera leo)

2009 ◽  
Vol 185 (1) ◽  
pp. 115-146 ◽  
Author(s):  
G. L. Smuts ◽  
J. L. Anderson ◽  
J. C. Austin
Keyword(s):  
Age Determination ◽  
Panthera Leo ◽  
African Lion
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