scholarly journals Early stages of sex chromosome differentiation in fish as analysed by simple repetitive DNA sequences

Chromosoma ◽  
1992 ◽  
Vol 101 (5-6) ◽  
pp. 301-310 ◽  
Author(s):  
Indrajit Nanda ◽  
Manfred Schartl ◽  
Wolfgang Feichtinger ◽  
J�rg T. Epplen ◽  
Michael Schmid
Keyword(s):  
Repetitive Dna ◽  
Dna Sequences ◽  
Sex Chromosome ◽  
Repetitive Dna Sequences ◽  
Chromosome Differentiation ◽  
Early Stages

scholarly journals Tracking the evolution of sex chromosome systems in Melanoplinae grasshoppers through chromosomal mapping of repetitive DNA sequences

2013 ◽  
Vol 13 (1) ◽  
pp. 167 ◽  
Author(s):  
Octavio M Palacios-Gimenez ◽  
Elio R Castillo ◽  
Dardo A Martí ◽  
Diogo C Cabral-de-Mello
Keyword(s):  
Repetitive Dna ◽  
Dna Sequences ◽  
Sex Chromosome ◽  
Chromosomal Mapping ◽  
Repetitive Dna Sequences ◽  
Evolution Of Sex

scholarly journals Cloning and physical localization of male-biased repetitive DNA sequences in Spinacia oleracea (Amaranthaceae)

2021 ◽  
Vol 15 (2) ◽  
pp. 101-118
Author(s):  
Jian Zhou ◽  
Shaojing Wang ◽  
Li'ang Yu ◽  
Ning Li ◽  
Shufen Li ◽  
...  
Keyword(s):  
Repetitive Dna ◽  
Dna Sequences ◽  
Molecular Mechanisms ◽  
Chromosome Evolution ◽  
Sex Chromosome ◽  
Spinacia Oleracea ◽  
Repetitive Dna Sequences ◽  
Sex Chromosome Evolution ◽  
Single Chromosome ◽  
Degenerate Oligonucleotide

Spinach (Spinacia oleracea Linnaeus, 1753) is an ideal material for studying molecular mechanisms of early-stage sex chromosome evolution in dioecious plants. Degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR) technique facilitates the retrotransposon-relevant studies by enriching specific repetitive DNA sequences from a micro-dissected single chromosome. We conducted genomic subtractive hybridization to screen sex-biased DNA sequences by using the DOP-PCR amplification products of micro-dissected spinach Y chromosome. The screening yielded 55 male-biased DNA sequences with 30 576 bp in length, of which, 32 DNA sequences (12 049 bp) contained repeat DNA sequences, including LTR/Copia, LTR/Gypsy, simple repeats, and DNA/CMC-EnSpm. Among these repetitive DNA sequences, four DNA sequences that contained a fragment of Ty3-gypsy retrotransposons (SP73, SP75, SP76, and SP77) were selected as fluorescence probes to hybridization on male and female spinach karyotypes. Fluorescence in situ hybridization (FISH) signals of SP73 and SP75 were captured mostly on the centromeres and their surrounding area for each homolog. Hybridization signals primarily appeared near the putative centromeres for each homologous chromosome pair by using SP76 and SP77 probes for FISH, and sporadic signals existed on the long arms. Results can be served as a basis to study the function of repetitive DNA sequences in sex chromosome evolution in spinach.

scholarly journals Cloning and physical localization of male-biased repetitive DNA sequences in Spinacia oleracea (Amaranthaceae)

2021 ◽  
Vol 15 (2) ◽  
pp. 101-118
Author(s):  
Jian Zhou ◽  
Shaojing Wang ◽  
Li'ang Yu ◽  
Ning Li ◽  
Shufen Li ◽  
...  
Keyword(s):  
Repetitive Dna ◽  
Dna Sequences ◽  
Molecular Mechanisms ◽  
Chromosome Evolution ◽  
Sex Chromosome ◽  
Spinacia Oleracea ◽  
Repetitive Dna Sequences ◽  
Sex Chromosome Evolution ◽  
Single Chromosome ◽  
Degenerate Oligonucleotide

Spinach (Spinacia oleracea Linnaeus, 1753) is an ideal material for studying molecular mechanisms of early-stage sex chromosome evolution in dioecious plants. Degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR) technique facilitates the retrotransposon-relevant studies by enriching specific repetitive DNA sequences from a micro-dissected single chromosome. We conducted genomic subtractive hybridization to screen sex-biased DNA sequences by using the DOP-PCR amplification products of micro-dissected spinach Y chromosome. The screening yielded 55 male-biased DNA sequences with 30 576 bp in length, of which, 32 DNA sequences (12 049 bp) contained repeat DNA sequences, including LTR/Copia, LTR/Gypsy, simple repeats, and DNA/CMC-EnSpm. Among these repetitive DNA sequences, four DNA sequences that contained a fragment of Ty3-gypsy retrotransposons (SP73, SP75, SP76, and SP77) were selected as fluorescence probes to hybridization on male and female spinach karyotypes. Fluorescence in situ hybridization (FISH) signals of SP73 and SP75 were captured mostly on the centromeres and their surrounding area for each homolog. Hybridization signals primarily appeared near the putative centromeres for each homologous chromosome pair by using SP76 and SP77 probes for FISH, and sporadic signals existed on the long arms. Results can be served as a basis to study the function of repetitive DNA sequences in sex chromosome evolution in spinach.

scholarly journals Repetitive DNA sequences near three humanβ-type globin genes

1980 ◽  
Vol 8 (15) ◽  
pp. 3319-3333 ◽  
Author(s):  
Lesley W. Coggins ◽  
G.Joan Grindlay ◽  
J.Keith Vass ◽  
Alison A. Slater ◽  
Paul Montague ◽  
...  
Keyword(s):  
Repetitive Dna ◽  
Dna Sequences ◽  
Repetitive Dna Sequences ◽  
Globin Genes

Fluorescence in situ hybridization on plant extended chromatin DNA fibers for single-copy and repetitive DNA sequences

2011 ◽  
Vol 30 (9) ◽  
pp. 1779-1786 ◽  
Author(s):  
Kun Yang ◽  
Hecui Zhang ◽  
Richard Converse ◽  
Yong Wang ◽  
Xiaoying Rong ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Repetitive Dna ◽  
Dna Sequences ◽  
Single Copy ◽  
Repetitive Dna Sequences
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