Electron microscopic identification of sex chromatin bodies of tissue culture cells

During a study of the development of infectious laryngotracheitis (LT) virus in tissue culture cells, unusual tubular formations were found in the cytoplasm of a small proportion of the affected cells. It is the purpose of this report to describe the morphologic characteristics of the tubules and to discuss their possible association with the development of virus.The source and maintenance of the strain of LT virus have been described. Prior to this study, the virus was passed several times in chicken embryo kidney (CEK) tissue culture cells.

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A Method for Electron Microscopic Study of Tissue Culture Cell Monolayers Grown in Tubes

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100060672 ◽

1967 ◽

Vol 25 ◽

pp. 132-133

Author(s):

R. Stephens ◽

G. Schidlovsky ◽

S. Kuzmic ◽

P. Gaudreau

Keyword(s):

Electron Microscopy ◽

Tissue Culture ◽

Light Microscopy ◽

Cell Sheet ◽

Culture Cell ◽

Tissue Culture Cell ◽

Electron Microscopic ◽

Cell Sheets ◽

Morphological Alterations ◽

Culture Cells

The usual method of scraping or trypsinization to detach tissue culture cell sheets from their glass substrate for further pelletization and processing for electron microscopy introduces objectionable morphological alterations. It is also impossible under these conditions to study a particular area or individual cell which have been preselected by light microscopy in the living state.Several schemes which obviate centrifugation and allow the embedding of nondetached tissue culture cells have been proposed. However, they all preserve only a small part of the cell sheet and make use of inverted gelatin capsules which are in this case difficult to handle.We have evolved and used over a period of several years a technique which allows the embedding of a complete cell sheet growing at the inner surface of a tissue culture roller tube. Observation of the same cell by light microscopy in the living and embedded states followed by electron microscopy is performed conveniently.

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Sulfhydryl bond formation is a prerequisite for proper cycling of centrosomes and chromosomes in mammalian tissue culture cells

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100147557 ◽

1993 ◽

Vol 51 ◽

pp. 342-343

Author(s):

Heide Schatten ◽

Neidhard Paweletz ◽

Ron Balczon

Keyword(s):

Tissue Culture ◽

Cell Cycle Progression ◽

Group Formation ◽

Sulfhydryl Group ◽

Mammalian Tissue ◽

Mitotic Chromosomes ◽

Microtubule Network ◽

Culture Cells ◽

Tissue Culture Cells ◽

Transmission Electron

To study the role of sulfhydryl group formation during cell cycle progression, mammalian tissue culture cells (PTK2) were exposed to 100¼M 2-mercaptoethanol for 2 to 6 h during their exponential phase of growth. The effects of 2-mercaptoethanol on centrosomes, chromosomes, microtubules, membranes and intermediate filaments were analyzed by transmission electron microscopy (TEM) and by immunofluorescence microscopy (IFM) methods using a human autoimmune antibody directed against centrosomes (SPJ), and a mouse monoclonal antibody directed against tubulin (E7). Chromosomes were affected most by this treatment: premature chromosome condensation was detected in interphase nuclei, and the structure in mitotic chromosomes was altered compared to control cells. This would support previous findings in dividing sea urchin cells in which chromosomes are arrested at metaphase while the centrosome splitting cycle continues. It might also support findings that certairt-sulfhydryl-blocking agents block cyclin destruction. The organization of the microtubule network was scattered probably due to a looser organization of centrosomal material at the interphase centers and at the mitotic poles.

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Trypsin action on the growth of Sendai virus in tissue culture cells. IV. Evidence for activation of sendai virus by cleavage of a glycoprotein.

Journal of Virology ◽

10.1128/jvi.18.3.1147-1150.1976 ◽

1976 ◽

Vol 18 (3) ◽

pp. 1147-1150 ◽

Cited By ~ 21

Author(s):

M Ohuchi ◽

M Homma

Keyword(s):

Tissue Culture ◽

Sendai Virus ◽

Culture Cells ◽

Tissue Culture Cells

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UDP-D-glucose 4-epimerase activity of the tissue culture of white poplars (Populus alba L. var. pyramidalis)

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19821530 ◽

1982 ◽

Vol 47 (5) ◽

pp. 1530-1536 ◽

Cited By ~ 1

Author(s):

Ladislav Bilisics ◽

Štefan Karácsonyi ◽

Marta Kubačková

Keyword(s):

Tissue Culture ◽

Cell Walls ◽

Enzyme Preparation ◽

Uridine Diphosphate ◽

Populus Alba ◽

Activity Change ◽

White Poplar ◽

Culture Cells ◽

Tissue Culture Cells ◽

Galactose Content

The presence of UDP-D-glucose 4-epimerase (EC 5.1.3.2) in the culture tissue of white poplar was evidenced. As found, the partially purified enzyme preparation contained UDP-D-glucose glucosyltransferase, UDP-D-galactose galactosyltransferase and non-specific enzymes able to cleave the uridine-diphosphate saccharides into the appropriate hexose monophosphates. The activity change of UDP-D-glucose 4-epimerase in tissue culture cells during the growth was in accord with changes in D-galactose content in cell walls and indicated the possibility to regulate the formation of polysaccharides containing D-galactose at the level of production of UDP-D-galactose in cells.

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